朱娟娟,马海军,覃洪云,徐小云,张琇.解钾菌与盐胁迫对枸杞幼苗根系特征和生理代谢的影响[J].干旱地区农业研究,2021,39(5):50~58
解钾菌与盐胁迫对枸杞幼苗根系特征和生理代谢的影响
]Effects of potassium\|solubilizing bacteria and salt stress on root property and physiological metabolism of Lycium barbarum seedlings
  
DOI：10.7606/j.issn.1000-7601.2021.05.06
中文关键词:  解钾菌  盐胁迫  枸杞幼苗  根系特征  生理代谢
英文关键词:potassium\|solubilizing bacteria  salt stress  Lycium barbarum seedlings  root characteristics  physiological metabolism
基金项目:宁夏自然科学基金（2018AAC03104）；宁夏回族自治区重点研发计划重点项目（2018BBF02019）
作者单位
朱娟娟 北方民族大学生物科学与工程学院，宁夏 银川 750021；宁夏葡萄与葡萄酒技术创新中心，宁夏 银川 750021 
马海军 北方民族大学生物科学与工程学院，宁夏 银川 750021；宁夏葡萄与葡萄酒技术创新中心，宁夏 银川 750021 
覃洪云 北方民族大学生物科学与工程学院，宁夏 银川 750021 
徐小云 北方民族大学生物科学与工程学院，宁夏 银川 750021 
张琇 北方民族大学生物科学与工程学院，宁夏 银川 750021；宁夏特殊生境微生物资源开发与利用重点实验室，宁夏 银川 750021 
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中文摘要:
      以‘宁杞1号’为试验试材，采用双因素裂区设计，以盐胁迫为主因素（100 mmol·L-1 NaCl），以解钾菌（B1，B2，B3）为副因素，对枸杞幼苗根系总长度、投影面积、表面积、体积、平均直径、根尖数、根系活力、活性氧含量、可溶性糖含量和超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、过氧化物酶（POD）、谷胱甘肽过氧化物酶（GSH-Px）活性及蔗糖磷酸合成酶 (SPS)、蔗糖合成酶（SS）和转化酶（INV）活性进行测定，综合评价不同处理中枸杞根系的生长状况。结果表明，解钾菌与盐胁迫对枸杞幼苗根系超氧阴离子（O—·2）含量和SOD、CAT、POD活性均无显著性影响，而对其他指标具有明显的影响。不同处理组合中，B1处理枸杞幼苗根系总长度最长，分别比CK（不添加NaCl和菌剂）、B2、B3、NaCl-CK、NaCl-B1、NaCl-B2、NaCl-B3处理长101%、89%、65%、112%、105%、122%、181%。B2处理中枸杞根系的投影面积、表面积和体积最大及转化酶活性最高；NaCl-B1处理中枸杞幼苗根尖数最大，根系活力最强，GSH-Px和SS活性最高；NaCl-B2处理中枸杞幼苗根系可溶性糖含量和SPS活性最高；NaCl-B3处理中枸杞幼苗根系H2O2含量最低，分别比ck、NaCl-CK、B1、NaCl-B1、B2、NaCl-B2、B3处理低24.3%、34.3%、3.9%、41.1%、17.3%、36.3%、14.3%。通过对枸杞幼苗根系17项指标进行综合评价，排在前三名的依次为NaCl-B1、B2、NaCl-B2处理。因此，解钾菌对枸杞幼苗根系的生长发育具有促进作用，无论是否存在盐胁迫，接种B1或B2单一菌种效果均优于其混合菌种B3。盐胁迫下，接种B1解钾菌更有利于枸杞根系的生长发育；非盐胁迫下，接种B2解钾菌更有利于枸杞根系的生长发育。
英文摘要:
      Cultivar Ningqi 1 was used to investigate the regulating effects of salt stress (100 mmol·L-1 NaCl) and potassium\|solubilizing bacteria (B1, B2, B3) on total root length, root projected area, root surface area, root volume, root average diameter, root tip number, root activity, superoxide anion (O—·2) content, hydrogen peroxide (H2O2) content, soluble sugar content and superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), glutathione peroxidase (GSH-PX), as well as sucrose phosphate synthase (SPS), sucrose synthase (SS), and invertase (INV) activity of Lycium barbarum seedlings. Double factor fracture zone test design was employed to evaluate comprehensively the root growth status of Lycium barbarum seedlings in different treatments with salt stress as the main factor and potassium\|solubilizing bacteria as the blessing factor. Results showed that potassium\|solubilizing bacteria and salt stress had no significant effect on the content of superoxide anion (O—·2), the activity of SOD, CAT, and POD in the roots of Lycium barbarum seedlings, but had significant effect on other indicators. Among different treatment combinations, the root length of Lycium barbarum seedlings was the longest in B1 treatment, which was longer by 101%, 89%, 65%, 112%, 105%, 122%, and 181% than that of ck, B2, B3, NaCl-CK, NaCl-B1, NaCl-B2, and NaCl-B3 treatments, respectively. The projected area, surface area, volume, and invertase activity of roots were the highest in B2 treatment. The root tip number, root activity, GSH-PX activity and SS activity were the highest in NaCl-B1 treatment. The soluble sugar content and SPS activity were the highest in NaCl-B2 treatment. The H2O2 content in the roots of Lycium barbarum seedlings was the lowest in NaCl-B3 treatment, which was lower by 24.3%, 34.3%, 3.9%, 41.1%, 17.3%, 36.3%, and 14.3% than that of ck, NaCl-CK, B1, NaCl-B1, B2, NaCl-B2, and B3 treatments, respectively. The comprehensive evaluation score by principal component analysis showed that the top three treatments were NaCl-B1, B2, and NaCl-B2. Generally, potassium\|solubilizing bacteria promoted the growth and development of the root system of Lycium barbarum seedlings. Whether under salt stress or not, inoculation of single strains B1 or B2 was better than that of mixed strains （B3）. Inoculation of potassium\|releasing bacteria B1 was more conducive to the growth and development of Lycium barbarum roots under salt stress. Inoculation of B2 potassium\|solubilizing bacteria was more conducive to the growth and development of the roots of Lycium barbarum seedlings under non\|salt stress.
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