| 刘翔,左凯峰,许伟,郭娜,秦梦凡,徐宇,马宁,李青青,张淼,李浩东,王茸茸,黄镇,徐爱遐.PEG 6000模拟干旱胁迫下甘蓝型油菜芽期及苗期抗旱指标筛选[J].干旱地区农业研究,2021,39(5):66~70 |
| PEG 6000模拟干旱胁迫下甘蓝型油菜芽期及苗期抗旱指标筛选 |
| Selecting drought resistance indexes of Brassica napus L. under PEG 6000 simulated drought stress at germination and seedling stage |
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| DOI：10.7606/j.issn.1000-7601.2021.05.08 |
| 中文关键词: 甘蓝型油菜 PEG 6000 抗旱指标 芽期 苗期 |
| 英文关键词:Brassica napus L. PEG 6000 drought resistance index germination stage seedling stage |
| 基金项目:“十三五”国家重点研发计划（2018YFD0100600;2016YFD0101300）；国家科技重大专项（2018ZX08020001-011） |
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| 中文摘要: |
| 采用聚乙二醇（PEG 6000）模拟干旱胁迫，分析干旱胁迫下8份甘蓝型油菜芽期和苗期抗旱相关指标，鉴定芽期和苗期抗旱性，筛选抗旱评价指标。结果表明，甘蓝型油菜种质芽期和苗期抗旱性强弱不同，芽期抗旱性鉴定的最适PEG 6000浓度为15%，成苗率可作为芽期抗旱性的鉴定指标。通过主成分分析、隶属函数等方法分析苗期相关指标的变化，利用抗旱性综合度量值D值评价甘蓝型油菜苗期抗旱性，结果表明叶片相对含水量（r=0.907**）、可溶性蛋白（r=0.921**）与抗旱度量值D值呈极显著正相关，丙二醛含量（r=-0.837**）与D值呈极显著负相关，这些指标可作为甘蓝型油菜苗期抗旱性的评价指标。 |
| 英文摘要: |
| Polyethylene glycol 6000 (PEG 6000) was used to simulate drought stress, analyze the drought resistance related indexes of eight Brassica napus L. germplasms at germination and seedling stage, identify drought resistance of test materails and select for drought resistance evaluation indexes. The results showed that the drought resistance of Brassica napus L. was different at germination stage and seedling stage. The optimum PEG 6000 concentration for drought resistance identification at germination stage was 15%, and the seedling rate could be used as an indicator of drought resistance at germination stage. Component analysis, membership function and other methods were used to analyze the changes of relevant indicators at seedling stage. The drought resistance of Brassica napus L. at seedling stage was evaluated by the comprehensive measure of drought resistance D value. The results showed that the relative water content of leaves (r=0.907**) and soluble protein (r=0.921**) were significantly positively correlated with D value, and malondialdehyde content was significantly negatively correlated with the D value (r=-0.837**). These indicators could be used as drought resistance evaluation indicators in seedling stage of Brassica napus L. |
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