孙嘉华,李雨萌,凌翰,惠竹梅.2,4-表油菜素内酯对多菌灵处理下葡萄抗氧化代谢系统的影响[J].干旱地区农业研究,2021,(5):71~75
2,4-表油菜素内酯对多菌灵处理下葡萄抗氧化代谢系统的影响
Effects of 2,4-epibrassinolide on antioxidant system of grape seedlings treated with carbendazim
  
DOI:10.7606/j.issn.1000-7601.2021.05.09
中文关键词:  酿酒葡萄  2,4-表油菜素内酯  多菌灵  抗氧化代谢
英文关键词:wine grape  2,4-epibrassinolide  carbendazim  antioxidant system
基金项目:国家重点研发计划项目(2017YFC1502806-4);国家现代农业(葡萄)产业技术体系建设专项资助项目(CARS-29-zp-6)
作者单位
孙嘉华 College of Enology, Northwest A & F University, Yangling, Shaanxi 712100, China 
李雨萌 College of Enology, Northwest A & F University, Yangling, Shaanxi 712100, China 
凌翰 College of Enology, Northwest A & F University, Yangling, Shaanxi 712100, China 
惠竹梅 College of Enology, Northwest A & F University, Yangling, Shaanxi 712100, China 
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中文摘要:
      以欧亚种酿酒葡萄(Vitis vinifera L.)赤霞珠(Cabernet Sauvignon)2 a生葡萄盆栽苗为试材,研究喷施外源2,4-表油菜素内酯(EBR)对多菌灵(CBD)处理下葡萄叶片过氧化氢(H2O2)、丙二醛(MDA)、超氧阴离子(O—·2)含量、抗氧化物质(GSH和AsA)、抗氧化酶(CAT、POD、GR)活性的影响。试验设3个处理,即:CK(清水)、稀释800倍CBD、0.3 mg·L-1 EBR+稀释800倍CBD,结果表明:与对照相比,CBD处理使葡萄叶片H2O2、MDA和O—·2含量显著升高,平均比对照分别升高50.09%、35.58%、60.06%;葡萄叶片抗氧化物质GSH、AsA的含量与对照相比无显著差异;CBD处理使葡萄叶片抗氧化酶CAT、POD、GR的活性显著增强,平均比对照分别升高24.17%、63.51%、8.44%。与单独CBD处理相比, EBR+CBD处理使葡萄叶片H2O2、MDA、O—·2含量显著降低,平均比单独CBD处理分别降低18.49%、14.44%、17.55%;同时使葡萄叶片抗氧化物质GSH、AsA含量显著升高,平均比单独CBD处理分别升高5.46%、24.92%;EBR+CBD处理使葡萄叶片抗氧化酶CAT、GR的活性显著增强,平均比单独CBD处理分别高17.15%和5.33%,但对POD活性的作用效果不明显。因此,0.3 mg·L-1外源EBR可能通过提高葡萄植株还原型抗氧化物质含量以及CAT、GR活性来降低植株内源活性氧、自由基水平,缓解农药对葡萄植株的伤害,可作为预处理试剂在生产中应用。
英文摘要:
      This study was conducted to determine the influence of exogenous EBR (2,4-Epibrassinolide) on antioxidant system of grape seedlings treated with carbendazim (CBD). With wine grape variety Cabernet Sauvignon (Vitis vinifera L.) biennial potted seedlings as the experimental material, effects of the exogenous EBR on reactive oxygen species (H2O2, O—·2), malondialdehyde content (MDA), antioxidant substances content (ASA, GSH) and antioxidant enzyme activities (CAT, POD, GR) of grape leaves were investigated. There were three treatments: CK(water), 800 times diluted CBD, 0.3 mg·L-1 EBD+800 times diluted CBD. As compared with control, CBD treatment increased the content of H2O2, MDA, and O—·2 in grape leaves, and on average it increased by 50.09%, 35.58%, and 60.06%, respectively. There was no significant difference in the content of antioxidant substances GSH and AsA in grape leaves when compared to control group. In comparison with control, CBD treatment caused significant enhance of CAT, POD, and GR activities in grape leaves, and on average it increased by 24.17%, 63.51%, 8.44%, respectively. When compared to CBD treatment alone, EBR+CBD treatment significantly decreased the content of H2O2, MDA, and O—·2, averagely decreased by 18.49%, 14.44%, and 17.55%, respectively. EBR+CBD treatment significantly increased the content of ASA and GSH in grape leaves compared to CBD treatment alone, and the average increases were 5.46% and 24.92%, respectively. In comparison with CBD treatment alone, EBR+CBD treatment caused significant enhance of CAT and GR activities in grape leaves, averagely increased by 17.15%, 5.33%, respectively, while did not cause an obvious effect on POD activity. Therefore, 0.3 mg·L-1 exogenous EBR decreased the level of endogenous reactive oxygen species and free radicals by probably increasing the content of reduced antioxidants and the activities of CAT and GR in grape plants to alleviate the damage of pesticides to grape plants. This method can be used as a pretreatment in grape production.
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