王立光,叶春雷,陈军,李进京,李静雯,罗俊杰.胡麻-小麦轮作更替土壤的细菌群落多样性分析[J].干旱地区农业研究,2021,(5):84~89
胡麻-小麦轮作更替土壤的细菌群落多样性分析
Diversity analysis of soil bacterial community in oil flax\|wheat rotation system
  
DOI:10.7606/j.issn.1000-7601.2021.05.11
中文关键词:  胡麻-小麦轮作  土壤细菌群落  高通量测序  多样性分析  Tax4Fun功能预测
英文关键词:oil flax\|wheat rotation  soil bacteria community  high\|throughput sequencing  diversity analysis  Tax4Fun function prediction
基金项目:甘肃省农业科学院博士基金(2019GAAS29);甘肃省科技计划资助项目-自然科学基金(20JR5RA106);财政部和农业农村部:国家现代农业产业技术体系资助项目(CARS\|14\|2\|23) ;国家自然科学基金(31660391);甘肃省农业科学院科技支撑计划(2018GAAS01);甘肃省农业科学院中青年基金(2017GAAS93;2019GAAS37;2020GAAS43)
作者单位
王立光 Biotechnology Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou, Gansu 730070, China 
叶春雷 Biotechnology Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou, Gansu 730070, China 
陈军 Biotechnology Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou, Gansu 730070, China 
李进京 Biotechnology Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou, Gansu 730070, China 
李静雯 Biotechnology Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou, Gansu 730070, China 
罗俊杰 Biotechnology Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou, Gansu 730070, China 
摘要点击次数: 183
全文下载次数: 135
中文摘要:
      采集胡麻-小麦轮作更替土壤,分别为第一年胡麻种植土壤(TC1)、第二年小麦轮作土壤(TR)和第三年胡麻种植土壤(TC2),利用高通量测序技术对不同土壤细菌群落进行测定,旨在揭示胡麻-小麦轮作更替对土壤细菌群落结构和多样性的影响。结果表明,TC2根际土壤样本细菌OTU数目最多,为1778条,细菌群落丰度指数(Chao指数1700.02和Ace指数1689.03)最低,而细菌群落多样性指数中,shannon指数表现为TC1(6.51)>TR(6.42)>TC2(6.21),simpson指数表现为TC2(0.0088)>TR(0.0049)>TC1(0.0030),即随着更替年限而降低。3种不同土壤样本内共获得细菌33门,且优势菌门(相对丰度>0.01)均为变形菌门(Proteobacteria)、酸杆菌门(Acidobacteria)、放线菌门(Actinobacteria)和绿湾菌门(Chloroflexi)等11个菌门,各优势菌门在各处理中所占的丰度存在差异。随着种植更替,变形菌门(TC1 31.65%, TR 24.52%, TC2 28.53%)和芽单胞菌门(TC1 8.04%, TR 5.41%, TC2 6.01%)丰度先降低再升高,酸杆菌门(TC1 19.36%, TR 26.13%, TC2 11.18%)和绿湾菌门(TC1 9.69%, TR 12.68%, TC2 10.53%)丰度先增加后降低,放线菌门(TC1 12.14%, TR 13.79%, TC2 22.82%)丰度逐渐增加。细菌群落??多样性分析显示,轮作使土壤细菌的群落结构与TC1保持较小差异,而后续胡麻种植使其发生较大变化。通过Tax4Fun法在更替土壤中检测到细胞新陈代谢、遗传信息加工和环境信息处理等基因功能。大部分功能基因在TR的丰度与TC1相近。这些结果说明,小麦轮作能降低土壤细菌多样性和细菌群落结构组成的改变程度,从而利于下茬胡麻的种植。
英文摘要:
      In order to reveal the effect of flax\|wheat rotation on the structure and diversity of soil bacterial community, the high\|throughput sequencing technique was used to determine the bacterial community indifferent soil collected. The results showed that the number of bacteria OTU (1778) was the highest, and the abundance index(Chao index 1700.02 and Ace index 1689.03)of bacteria community was the lowest in the TC2 soil samples. The diversity of bacterial community (Shannon index TC1 (6.51)>TR(6.42)>TC2(6.21), Simpson index TC2 (0.0088)>TR(0.0049)>TC1(0.0030)) decreased with replacement years. A total of 33 phylum of bacteria were isolated from the different soil samples. The 11-dominant phylum(>0.01)were the same in different samples, including proteobacteria, acidobacteria, actinobacteria and chloflexi, etc. The abundance of the dominant phylum was different in each sample. With the replacement of planting, the abundance of proteobacteria (TC1 31.65%, TR 24.52%, TC2 28.53%) and gemmatimonadetes (TC1 8.04%, TR 5.41%, TC2 6.01%) decreased first and then increased. The abundance of acidobacteria (TC1 19.36%, TR 26.13%, TC2 11.18%) and chloroflexi (TC1 9.69%, TR 12.68%, TC2 10.53%) increased first and then decreased. The abundance of actinobacteria (TC1 12.14%, TR 13.79%, TC2 22.82%) increased gradually. Beta diversity analysis of bacterial community showed that wheat rotation had little effect on the structure of soil bacterial community like TC1, but the follow\|up cultivation of oil flax had more effect. Cell metabolism, genetic information processing and environmental information processing were detected by Tax4Fun method in different soil collected. The abundance of most functional genes in TR was similar to that of TC1. All the results in this experiment indicated that wheat rotation helped maintain diversity and structure of soil bacteria community and change soil bacteria community slightly, so as to benefit the cultivation of subsequently oil flax. These results provided theoretical basis for the mechanism analysis of relieving continuous cropping obstacle of oil flax by rotation.
查看全文  查看/发表评论  下载PDF阅读器