| 石晓英,王荣,姬新梅,王健强,徐继忠,张学英,周莎莎.干旱胁迫下不同中间砧对苹果苗生理特性及MdPIP1-1基因表达的影响[J].干旱地区农业研究,2021,39(6):73~78 |
| 干旱胁迫下不同中间砧对苹果苗生理特性及MdPIP1-1基因表达的影响 |
| Effects of different interstocks on physiological characteristics and MdPIP1-1 gene expression of grafted apple trees under drought stress |
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| DOI：10.7606/j.issn.1000-7601.2021.06.08 |
| 中文关键词: 苹果 中间砧 抗旱性 生理特性 MdPIP1-1基因 |
| 英文关键词:apple trees interstock drought resistance physiological characteristics MdPIP1-1 gene |
| 基金项目:河北省重点研发计划项目（19226317D）；河北省现代农业产业技术体系水果创新团队项目（HBCT2018100403）；国家现代农业产业技术体系建设专项资金项目（CARS-27） |
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| 中文摘要: |
| 为探讨不同中间砧对‘天红2号’红富士苹果苗抗旱性的影响，以2 a生‘天红2号/黄6/平邑甜茶’、‘天红2号/SH40/平邑甜茶’、‘天红2号/GM256/平邑甜茶’盆栽苗为试材，研究了干旱胁迫下不同中间砧对苹果苗旱害指数、相关生理指标及水通道蛋白基因MdPIP1-1相对表达量的影响。结果表明，随着干旱胁迫时间延长，土壤含水量降低，3种中间砧苗旱害指数、O-2产生速率升高，Fv/Fm值降低，SOD、POD活性呈现先升高再降低的趋势，但3个砧穗组合各指标的变化幅度不同。干旱9 d时3个砧穗组合以‘天红2号/GM256/平邑甜茶’的旱害指数最高，为91.67%，其Fv/Fm值与正常供水相比显著下降11.47%；‘天红2号/黄6/平邑甜茶’旱害指数仅为29.55%，Fv/Fm值下降幅度最小。干旱胁迫期间，3种中间砧苗的MdPIP1-1基因表达量均呈现先升高再降低的趋势，黄6中间砧苗的表达量峰值出现在第7 d，GM256和SH40中间砧苗出现在第5 d。综合认为，3个砧穗组合抗旱性强弱依次为‘天红2号/黄6/平邑甜茶’＞‘天红2号/SH40/平邑甜茶’＞‘天红2号/ GM256/平邑甜茶’。 |
| 英文摘要: |
| To explore the effects of different interstocks on drought resistance of Tianhong 2 apple trees, the two\|year\|old Tianhong 2/Huang 6/M.hupehensis,Tianhong 2/SH40/M.hupehensis, Tianhong 2/GM256/M.hupehensis potted apple trees were used as test materials to study effects of different interstocks on drought injury index and related physiological indexes and relative expression of aquaporin gene MdPIP1-1. The results showed that as drought stress time prolonged, soil water content and Fv/Fm decreased, and the drought injury index and O-2 production rate of the three interstock trees increased. And the activities of SOD and POD increased first and then decreased.The ranges of each index of the three rootstock combinations were different. On the 9th day of drought, the drought injury index of Tianhong 2/GM256/M.hupehensis reached the highest level of 91.67%, and its Fv/Fm decreased significantly by 11.47% when compared with normal water supply.The drought injury index of Tianhong 2/Huang 6/M.hupehensis was only 29.55%, and the decrease in Fv/Fm was the smallest. During the drought stress period, the expression of MdPIP1-1 gene in three interstock trees increased first and then decreased. The peak expression of Huang 6 appeared on the 7th day, and the expression of GM256 and SH40 appeared on the 5th day.In general, the drought resistance of the three rootstock combinations was in the order of Tianhong 2/Huang 6/M.hupehensis>Tianhong 2/SH40/M.hupehensis>Tianhong 2/GM256/M.hupehensis. |
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