| 王立光,叶春雷,陈军,李进京,罗俊杰.胡麻连作对土壤细菌群落的影响[J].干旱地区农业研究,2022,40(1):70~75 |
| 胡麻连作对土壤细菌群落的影响 |
| Effects of continuous cropping on bacteria community in oil flax soil |
| |
| DOI：10.7606/j.issn.1000-7601.2022.01.08 |
| 中文关键词: 胡麻 连作 高通量测序 土壤细菌群落 |
| 英文关键词:oil flax continuous cropping high\|through put sequencing soil bacterial community |
| 基金项目:甘肃省农业科学院博士基金（2019GAAS29）；甘肃省科技计划资助项目-自然科学基金（20JR5RA106）；财政部和农业农村部：国家现代农业产业技术体系资助(CARS-14-2-23) ；甘肃省农业科学院现代生物育种专项（2020GAAS08）；甘肃省农业科学院中青年基金（2020GAAS43，2017GAAS93） |
|
| 摘要点击次数: 649 |
| 全文下载次数: 451 |
| 中文摘要: |
| 采集种植1、2 a和3 a的胡麻土壤，利用高通量测序技术对不同胡麻连作年限土壤细菌群落进行测定，旨在揭示不同连作年限对胡麻土壤细菌群落结构和多样性的影响。结果表明：连作3 a土壤样本细菌OTU数目最多，为1 773条，细菌群落丰度指数（Chao指数1 759.07和Ace指数1 741.89）最高，而细菌群落多样性（Shannon指数T1(6.45）＞T2（6.41）＞T3(6.33),Simpson指数T1(0.0034）＜T2（0.0037）＜T3(0.0074))随着连作年限增长而 降低；不同连作年限胡麻土壤内共获得36门的细菌，且优势菌门（丰度占比＞0.01）都为变形菌门（Proteobacteria）、酸杆菌门（Acidobacteria）、放线菌门（Actinobacteria）和绿湾菌门（Chloroflexi）等12个菌门，但各优势菌门在各样本中所占的丰度不同;随着连作年限增加，变形菌门丰度（T1（31.74%）＞T2（27.69%）＞T3(25.21%）)逐渐降低，酸杆菌门丰度（T1 19.39%,T2 21.99%,T3 14.28%）和拟杆菌门丰度（Bacteroidetes, T1 7.29%,T2 8.72%,T3 2.59%）先增加后降低，放线菌门丰度（T1（12.16%）＜T2（15.38%）＜T3(24.12%）)和绿湾菌门丰度（T1（9.58%）＜T2（10.99%）＜T3(11.82%）)逐渐增加。土壤细菌群落β多样性分析显示，连作显著改变土壤细菌的群落结构，其影响随连作年限增长而增强。研究表明胡麻连作降低土壤细菌多样性，影响细菌群落结构组成。 |
| 英文摘要: |
| In this study, the samples of the soil being planted oil flax for one year, continuously planted for two or three years were collected to determine the influence of different continuous cropping years on the bacterial community and diversity in the soil.The results showed that the number of bacteria OUT （1773）and the abundance index（Chao index 1 759.07 and Ace index 1 741.89）of bacteria community were the highest after three\|year continuous cropping, but the diversity of bacterial community（Shannon index T1(6.45）＞T2（6.41）＞T3(6.33), Simpson index T1(0.0034）＜T2（0.0037）＜T3(0.0074))decreased with the increase of continuous cropping years. A total of 36 phylum of bacteria were isolated from the samples of oil flax soil with different continuous cropping years. The 12 dominant phylum（＞0.01）were the same in different samples. They were Proteobacteria, Acidobacteria, Actinobacteria and Chloflexi, etc.The abundance of the dominant phylum was different in each sample. With the continuous cropping time increased, the abundance of Proteobacteria（T1（31.74%）＞T2（27.69%）＞T3(25.21%）) gradually decreases, the abundance of Acidobacteria （T1 19.39%,T2 21.99%,T3 14.28%） and Bacteroidetes(T1 7.29%,T2 8.72%,T3 2.59%)increased first and then decreased. The abundance of Actinobacteria（T1（12.16%）＜T2（15.38%）＜T3(24.12%）) and Chloflexi（T1（9.58%）＜T2（10.99%）＜T3(11.82%）) gradually increased. Beta diversity analysis of bacterial community showed that continuous cropping significantly changed the structure of soil bacterial community, and the effect increased with continuous cropping years. All the findings in this experiment indicated that continuous cropping of oil flax decreased the diversity of soil bacteria and affected the structure of bacterial community. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| | |