程少波,杨巽喆,邹丽,吴丹丹,陆佳乐,周永红,张海琴.23份多花黑麦草萌发期抗旱性评价[J].干旱地区农业研究,2022,40(2):1~8
23份多花黑麦草萌发期抗旱性评价
Drought resistance evaluation of 23 ryegrass germplasms at germination stage
  
DOI：10.7606/j.issn.1000-7601.2022.02.01
中文关键词:  多花黑麦草  萌发期  抗旱性评价  PEG-6000
英文关键词:ryegrass  Lolium multiflorum  germination stage  drought resistance evaluation  PEG-6000
基金项目:国家自然科学基金项目（31870309）；四川省科技厅农业科技成果转化项目（2021NZZJ0010）；四川省阿坝州科技局项目（R21YYJSYJ0014）
作者单位
程少波 四川农业大学小麦研究所，四川 成都 611130 
杨巽喆 四川农业大学小麦研究所，四川 成都 611130 
邹丽 四川农业大学小麦研究所，四川 成都 611130 
吴丹丹 四川农业大学小麦研究所，四川 成都 611130；西南作物基因资源发掘与利用国家重点实验室，四川 成都 611130 
陆佳乐 四川农业大学草业科技学院，四川 成都 611130；西南作物基因资源发掘与利用国家重点实验室，四川 成都 611130 
周永红 四川农业大学小麦研究所，四川 成都 611130；西南作物基因资源发掘与利用国家重点实验室，四川 成都 611130 
张海琴 四川农业大学草业科技学院，四川 成都 611130；西南作物基因资源发掘与利用国家重点实验室，四川 成都 611130 
摘要点击次数: 1109
全文下载次数: 867
中文摘要:
      为探究不同倍性多花黑麦草的抗旱能力，同时筛选优异种质资源，以23份不同倍性多花黑麦草为材料，在PEG-6000模拟干旱条件下进行种子萌发期抗旱性评价，测定相对胚根长、相对胚芽长、相对发芽率等6项萌发生长指标，结合隶属函数分析、主成分分析和聚类分析对其抗旱性进行综合评价。结果表明：干旱处理下，四倍体多花黑麦草的根长和芽长均高于二倍体,分别高1.79倍和1.26倍。主成分分析表明：活力指数和根长分别为萌发因子和根部生长因子中的主要成分，载荷量分别为0.924和0.743。23份材料的D值变化范围为0.117～0.946，材料PI 238886的D值最低，PI 343155的D值最高。对D值进行聚类分析，筛选出四倍体抗旱材料5份（PI 343155、特高、929-3、川农1号、PI 619469），二倍体抗旱材料5份(PI 266111、PI 283610、PI 619471、PI 634251、W6 9271），中等材料9份，干旱敏感材料4份。
英文摘要:
      To explore the drought resistance ability of different ploidy ryegrass (Lolium multiflorum) and screen excellent germplasm resources, 23 ryegrass germplasms were used to evaluate drought resistance during seed germination under PEG-6000 simulated drought conditions in this study. Six indexes, such as relative root length, relative shoot length, and relative germination rate were measured. The drought tolerance of ryegrass was comprehensively evaluated by membership function analysis, principal component analysis and cluster analysis. The results showed that the root length and shoot length of tetraploid ryegrass were 1.79 times and 1.26 times higher than that of diploid ryegrass under drought treatment, respectively. Principal component analysis showed that vigor index and root length were the main components of germination factor and root growth factor, respectively, and the loading capacity was 0.924 and 0.743, respectively. The D value of 23 materials ranged from 0.117 to 0.946, among which the D value of PI 238886 was the lowest while that of PI 343155 was the highest. Five tetraploid drought resistant materials (PI 343155, Tetragold, 929-3, Chuannong No.1, PI 619469) and five diploid drought resistant materials (PI 266111, PI 283610, PI 619471, PI 634251, W6 9271) were selected by cluster analysis. Nine medium and 4 drought sensitive materials were also identified in this study.
查看全文  查看/发表评论  下载PDF阅读器