白明兴,庄泽龙,姬祥卓,彭云玲.转录组和iTRAQ技术联合揭示玉米根系的耐旱机制[J].干旱地区农业研究,2022,40(2):59~68
转录组和iTRAQ技术联合揭示玉米根系的耐旱机制
Research on drought\|tolerant regulatory mechanism of maize roots using transcriptome and iTRAQ techniques
  
DOI：10.7606/j.issn.1000-7601.2022.02.08
中文关键词:  玉米  根系  转录组  蛋白质组  关联分析  耐旱机制
英文关键词:maize  root system  transcriptome  proteome  joint analysis  drought\|tolerant mechanism
基金项目:国家级 抗旱高产优质玉米新品种育种繁植与推广委托项目(XZ20200810)
作者单位
白明兴 甘肃农业大学农学院,甘肃省干旱生境作物学重点实验室/甘肃省作物遗传改良与种质创新重点实验室, 甘肃 兰州 730070 
庄泽龙 甘肃农业大学农学院,甘肃省干旱生境作物学重点实验室/甘肃省作物遗传改良与种质创新重点实验室, 甘肃 兰州 730070 
姬祥卓 甘肃农业大学农学院,甘肃省干旱生境作物学重点实验室/甘肃省作物遗传改良与种质创新重点实验室, 甘肃 兰州 730070 
彭云玲 甘肃农业大学农学院,甘肃省干旱生境作物学重点实验室/甘肃省作物遗传改良与种质创新重点实验室, 甘肃 兰州 730070 
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中文摘要:
      采用RNA-Seq技术和iTRAQ技术对耐旱性不同的两玉米自交系（昌7-2和TS141）的幼苗根系差异基因(DEGs)和差异蛋白(DEPs)进行关联分析。定量关联分析结果表明，Chang 7-2 D1 vs CK1、Chang 7-2 D2 vs CK2、TS141 D1 vs CK1和TS141 D2 vs CK2等4个比较组中mRNA和蛋白表达趋势相同的基因相关系数分别为0.7993、0.5673、0.6406、0.6588，mRNA和蛋白表达趋势相反的基因相关系数分别为-0.7222、-0.5752、-0.7996、-0.7339；此外，通过对每个自交系的D1 vs CK1和D2 vs CK2关联结果进行整合后发现，在干旱胁迫下，昌7-2中mRNA和蛋白表达趋势相同的基因有19个，mRNA和蛋白表达趋势相反的基因有9个，TS141中mRNA和蛋白表达趋势相同的基因有11个，mRNA和蛋白表达趋势相反的基因有0个，表明植物根系在转录和转录后调控过程中存在差异。KEGG分析发现4个比较组所关联到的DEGs/DEPs主要涉及苯丙素的生物合成、次生代谢产物的生物合成和核糖体等，这些通路可能是不同自交系幼苗根系共同响应干旱胁迫的主要通路。在昌7-2和TS141中挑选9个DEPs/DEGs表达趋势相同的基因进行qRT-PCR分析表明，9个DEPs/DEGs的表达趋势与它们的 RNA-Seq和iTRAQ结果基本一致。
英文摘要:
      RNA-Seq and iTRAQ techniques were used to analyze the association of differentially expressed genes (DEGs) and differentially expressed proteins (DEPs) in seedling roots of two maize inbred lines, Chang 7-2 and TS141, with different drought tolerance. The results of quantitative correlation analysis showed that the correlation coefficients of genes with the same trend of mRNA and protein expression in Chang 7-2 D1 vs CK1, Chang 7-2 D2 vs CK2, TS141 D1 vs CK1 and TS141 D2 vs CK2 were 0.7993, 0.5673, 0.6406, and 0.6588, respectively. The correlation coefficients of mRNA and genes with opposite protein expression trend were -0.7222, -0.5752, -0.7996, and-0.7339, respectively. Further, by integrating the association of each self\|crossing D1 vs CK1 and D2 vs CK2, it was found that there were 19 genes with the same expression trend of mRNA and protein in Chang 7-2, 9 gene with opposite expression trend of mRNA and protein, 11 genes with the same expression trend of mRNA and protein in TS141, and 0 gene with opposite expression trend of mRNA and protein, indicating that there were differences in transcriptional and post\|transcriptional regulation in plant roots. KEGG analysis showed that the DEGs/DEPs associated with the four comparison groups were mainly related to phenylpropanoid biosynthesis, biosynthesis of secondary metabolites and ribosome, and these pathways might be the main pathways in response to drought stress in the roots of different inbred lines. 9 genes with the same expression trend of DEPs/DEGs were selected from Chang 7-2 and TS141 for qRT-PCR analysis, and the results showed that the expression trend of 9 DEPs/DEGs was basically consistent with their RNA-Seq and iTRAQ results.
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