| 蒋希瑶,牛宁,崔洪鑫,魏少伟,熊航,刘慧英,刁明.叶面喷施褪黑素对NaHCO3胁迫下番茄幼苗光合特性的影响[J].干旱地区农业研究,2022,40(2):87~95 |
| 叶面喷施褪黑素对NaHCO3胁迫下番茄幼苗光合特性的影响 |
| Effects of leaf surface melatonin spray on photosynthetic properties of tomato seedlings under NaHCO3 stress |
| |
| DOI：10.7606/j.issn.1000-7601.2022.02.11 |
| 中文关键词: 番茄幼苗 溶液培养 NaHCO3胁迫 褪黑素 光合 OJIP曲线 |
| 英文关键词:tomato seedlings solution culture NaHCO3 stress melatonin photosynthesis OJIP curve |
| 基金项目:国家自然科学基金（U19031） |
|
| 摘要点击次数: 945 |
| 全文下载次数: 604 |
| 中文摘要: |
| 采用营养液水培番茄幼苗的方式，研究了叶面喷施不同浓度褪黑素（MT）对NaHCO3胁迫（50 mmol·L-1）下‘里格尔87-5’加工番茄幼苗叶片的光合色素、光合气体交换参数和快速叶绿素荧光动力学特性的影响。结果表明：NaHCO3胁迫（MT0处理）导致番茄幼苗供体侧OEC（放氧复合体）和 PSII受体侧受到损伤，PSII反应中心活性、电子传递效率和光化学效率均下降，导致Pn、Gs分别降低了66.21%和82.36%。叶面喷施 100~400 μmol·L-1 MT可在不同程度上改变NaHCO3胁迫下的番茄幼苗OJIP曲线形状，降低K与J相趋势。MT0分别降低了番茄叶片的最大荧光（Fm）、最大量子效率（Fv/Fm）27.28%和18.53%，而100~400 μmol·L-1<、sup> MT分别提高了Fm与Fv/Fm 38%~56%与18.85%~21.22%；碱胁迫导致反应中心捕获电子传递到QA后的效率（Ψo）、光合性能指数(PIABS）分别降低了19.23%与78.27%，而MT处理使其分别提高了29.51%~47.80%、2.54~3.43倍，同时均使NaHCO3胁迫下单位反应中心的光能吸收量（ABS/RC）、热能量耗散（DIo/RC）、捕获能量（TRo/RC）降低了20.67%~26.79%和51.44%~57.38%，单位受光面积的光能吸收（ABS/CSo）、热耗散（DIo/CSo）的作用降低了0.65%~8.67%、39.02%~40.32%。说明100~400 μmol·L-1 MT处理可通过增强碱胁迫下电子传递速率和光反应活性，优化单位反应中心和单位受光面积的能量流分配，稳定和保护碱胁迫下番茄叶片的光合机构，最终提高其Pn，其中，尤以150 mmol·L-1 MT（MT150）处理的缓解效应最为显著，达到有效缓解碱胁迫对番茄幼苗的伤害作用。 |
| 英文摘要: |
| The effects of different concentrations of melatonin (MT) on photosynthetic pigments, photosynthetic gas exchange parameters and rapid chlorophyll fluorescence kinetics of leaves of ‘Rigel 87-5’ processed tomato seedlings under NaHCO3 stress (50 mmol·L-1) were studied. The results showed that NaHCO3 stress (MT0 treatment) caused damage to OEC (Oxygen releasing complex) and to PSII receptor side of tomato seedlings, decreased PSII reaction center activity, electron transfer efficiency and photochemical efficiency, and reduced Pn and Gs by 66.21% and 82.36%, respectively. Foliar spraying 100~400 μmol·L-1 MT could change the shape of OJIP curve of tomato seedlings under NaHCO3 stress and reduce the trend of K and J phase. MT0 reduced the maximum fluorescence (Fm) and maximum quantum efficiency (Fv/Fm) of tomato leaves by 27.28% and 18.53%, but 100~400 μmol·L-1 of MT increased Fm and Fv/Fm by 38%~56% and 18.85%~21.22%, respectively. Alkali stress led to the efficiency of electron capture in the reaction center after transfer to QA（Ψo). The photosynthetic performance index (PIABS) decreased by 19.23% and 78.27%， respectively, while MT treatment increased it by 29.51%~47.80% and 2.54~3.43 times, respectively. At the same time, the light energy absorption (ABS/RC), thermal energy dissipation (DIo/RC) and capture energy (TRo/RC) per unit reaction center under NaHCO3 stress decreased by 20.67%~26.79% and 51.44%~57.38%. The light energy absorption per unit light receiving area (ABS/CSo) and the effect of heat dissipation (DIO/CSo) decreased by 0.65%~8.67%, 39.02%~40.32%. The findings indicated that the 100~400 μmol·L-1 MT treatment enhanced the electron transfer rate and light reaction activity under alkali stress, optimized the energy flow distribution per unit reaction center and per unit light receiving area, stabilized and protected the photosynthetic mechanism of tomato leaves under alkali stress, and finally improved its Pn. Of which the 150 mmol·L-1 MT (MT150) had the most significant mitigation effect, which effectively alleviated the damage of alkali stress to tomato seedlings. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| | |