| 夏全超,张玉霞,孙明雪,陈卫东,孙昊,张庆昕,丛百明.磷肥下移深度对苜蓿根颈含氮保护物质及其抗寒性的影响[J].干旱地区农业研究,2022,40(2):96~101 |
| 磷肥下移深度对苜蓿根颈含氮保护物质及其抗寒性的影响 |
| Effects of phosphate fertilizer descending depth on nitrogen\|containing protective substances and cold resistance of alfalfa root crown |
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| DOI：10.7606/j.issn.1000-7601.2022.02.12 |
| 中文关键词: 苜蓿 磷肥下移 含氮保护物质 抗寒性 根颈 |
| 英文关键词:alfalfa phosphate fertilizers down nitrogen\|containing protective substances cold resistance root crown |
| 基金项目:内蒙古自然科学研究基金(2020MS03081);国家自然科学基金(31960352);内蒙古自治区科技计划项目(2021GG0109) |
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| 中文摘要: |
| 为探讨磷肥下移深度对苜蓿越冬器官生理特性的影响，本试验于2020年7月1日播种，以‘北极熊’紫花苜蓿品种为供试材料，设置5个磷肥下移深度处理：施地表、距离地表7.5、15 、22.5、30 cm（分别以S0、S1、S2、S3、S4表示），并以不施肥为对照（CK），于越冬期对苜蓿根颈进行模拟低温处理（4℃、-10℃、-20℃和-30℃），测定相对电导率、含氮保护物质含量（可溶性蛋白、脯氨酸、游离氨基酸含量）。结果表明：随着低温胁迫温度的下降，苜蓿根颈相对电导率表现为持续增长，可溶性蛋白、游离氨基酸表现为先升高后降低的趋势，且可溶性蛋白、脯氨酸和游离氨基酸含量均在-10℃下达到最大值，分别较4℃处理增长了65.18％～144.67％、46.24％～133.33％和27.13％～57.01％。苜蓿根颈相对电导率在S2施磷深度为15 cm处理下数值最小，较CK降低了24.87％～57.21％；苜蓿根颈可溶性蛋白含量在S2施磷深度为15 cm处理下数值最大，较CK及其他施磷深度处理增长了8.18％～79.81％；-10℃、-20℃处理下的苜蓿根颈游离氨基酸与脯氨酸含量，在S0、S1和S2处理下均显著高于CK处理（P＜0.05）。说明距离土壤表面15 cm（S2处理）施用磷肥最有利于通过提高轻度和中度低温胁迫下苜蓿含氮物质含量，降低其细胞的相对电导率，提高苜蓿抗寒能力。 |
| 英文摘要: |
| To explore the effects of the depth of descending phosphate fertilizer on physiological characteristics of the overwintering organs of alfalfa, the ‘Gibraltar’ alfalfa variety was sown on July 1, 2020. Five treatments of phosphate fertilizer depth included the ground surface, 7.5, 15, 22.5, 30 cm from the ground (respectively represented by S0, S1, S2, S3, S4), and no fertilization as the control (CK). Simulated low temperature treatment on the root crown of alfalfa during the overwintering period (4,-10,-20℃ and -30℃) was used to determine the relative conductivity, the content of nitrogen\|containing protective substances (soluble protein, proline, free amino acid content). The results showed that with the decrease of low temperature stress, the relative conductivity of alfalfa root crown displayed a continuous increase. The soluble protein and free amino acid had a trend of first increasing and then decreasing. The contents of soluble protein, proline and free amino acid were all reached the maximum value at -10℃, which increased by 65.18%～144.67%,46.24%～133.33% and 27.13%～57.01%, respectively, compared with 4℃ treatment. The relative conductivity of alfalfa root crown was the smallest under the treatment of S2 phosphorus application depth of 15 cm, which was reduced by 24.87%～57.21% compared with CK. The value of the soluble protein content of alfalfa root crown was the largest under the treatment of S2 phosphorus application depth of 15 cm, which was higher than that of CK and other treatments. The advanced phosphorus application treatment increased by 8.18%～79.81%. The proline and free amino acid content of the alfalfa root crown was significantly higher than other advanced phosphorus application treatments under the S0, S1 and S2 treatments, indicating that the application of phosphate fertilizer 15 cm (S2 treatment) from the soil surface was the most beneficial for increasing the nitrogen content of alfalfa, reducing the relative conductivity, and improving the cold resistance of alfalfa. |
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