| 陶吉杨,谭军利,郑飞龙,王西娜,张维江.宁南山区植被恢复模式对土壤主要酶活性、微生物多样性及土壤养分的影响[J].干旱地区农业研究,2022,40(3):207~217 |
| 宁南山区植被恢复模式对土壤主要酶活性、微生物多样性及土壤养分的影响 |
| Effects of vegetation recovery modes on major enzyme activities, microbial diversity, and nutrients in hilly soils of southern Ningxia |
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| DOI：10.7606/j.issn.1000-7601.2022.03.25 |
| 中文关键词: 植被恢复模式 土壤酶活性 土壤微生物多样性 土壤养分 |
| 英文关键词:vegetation recovery modes soil enzyme activity soil microbial diversity soil nutrients |
| 基金项目:宁夏回族自治区重点研发项目（2018BEG02010）；宁夏回族自治区自然科学基金项目（2020AAC03090）；国家自然科学基金项目（31860590）；宁夏回族自治区高等学校一流学科建设（水利工程）项目（NXYLXK2017A03） |
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| 中文摘要: |
| 探究不同植被恢复模式对土壤主要酶活性、微生物多样性及养分的影响，可为当地选择最适宜的植被恢复模式提供理论依据。利用野外取样与室内实验相结合的方法，研究了宁南山区4种植被恢复模式（7a生刺槐、落叶松、油松）以及荒地的土壤酶活性、微生物多样性、土壤养分特征，并利用Illumina-Miseq高通测序技术对土壤中细菌的16SrDNA基因V3~V4区片段和真菌的18SrDNA基因V4区片段进行了测序分析。结果表明:（1）在0~30 cm土层，荒地和刺槐土壤的脲酶、蔗糖酶活性较强，油松、落叶松的碱性磷酸酶、过氧化氢酶活性较强；（2）土壤真菌多样性对植被恢复模式的响应强于土壤细菌，土壤细菌的ACE、Chao l和Shannon指数高于土壤真菌，其多样性在4种植被恢复模式间无显著差异，油松显著提高了土壤真菌多样性；（3）荒地土壤全磷、碱解氮、速效磷、有机质含量最低，刺槐土壤的速效养分和有机质含量最高，土壤脲酶、过氧化氢酶与土壤养分相关性显著，土壤微生物多样性与土壤有机质、全磷含量密切相关。刺槐在改善多种土壤养分、酶活性上效果明显，是当地植被恢复可以优先考虑的树种。 |
| 英文摘要: |
| Exploring the influence of different vegetation recovery modes on soil major enzyme activities, microbial diversity, and nutrients can provide a theoretical basis for selecting the most appropriate vegetation recovery mode in the local area of southern Ningxia. The method of the combination of field sampling and laboratory experiments were used to study soil enzyme activity, microbial diversity and soil nutrient characteristics under three vegetation recovery modes (seven year’s Robinia pseudoacacia, Larix gmelinii (Rupr.) Kuzen., Pinus tabuliformis Carr.) and the wasteland in the hilly area soils of southern Ningxia. The Illumina-Miseqqualcomm sequencing technology was used to conduct the sequencing analyses for bacteria in the soil of 16 SrDNA gene segments of V3~V4 area and fungal 18 SrDNA gene segments of V4 area. The result showed: (1) In the 0~30 cm soil layer, the urease and sucrase activities of wasteland and Robinia pseudoacacia soil were stronger while the alkaline phosphatase and catalase activities of Pinus tabuliformis Carr. and Larix gmelinii (Rupr.) Kuzen. were stronger. (2) The response of soil fungal diversity to vegetation recovery model was stronger than that of soil bacteria. The ACE, Chaol and Shannon indexes of soil bacteria were higher than those of soil fungi. There was no significant difference in the diversity of soil fungi among the four recovery modes. The improvement effect of Pinus tabuliformis Carr. on soil fungal diversity was significant. (3) The contents of soil total phosphorus, alkali-hydrolytic nitrogen, available phosphorus and organic matter in wasteland were the lowest while the contents of available nutrients and organic matter in Robinia pseudoacacia were the highest. Soil urease and catalase activities were significantly correlated with soil nutrients, and soil microbial diversity was closely correlated with soil organic matter and total phosphorus contents. Robinia pseudoacacia can significantly improved soil nutrient status and enzyme activities, and therefore it could be considered as a species for local vegetation recovery. |
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