王子寅,刘秉儒,牟红霞,李子豪.荒漠草原不同发育阶段柠条灌丛堆土壤真菌群落多样性特征研究[J].干旱地区农业研究,2022,40(3):218~227
荒漠草原不同发育阶段柠条灌丛堆土壤真菌群落多样性特征研究
Diversity characteristics at different development stages of Caragana korshinskii Kom. Nebkhas in the desert steppe
  
DOI：10.7606/j.issn.1000-7601.2022.03.26
中文关键词:  柠条灌丛堆  土壤真菌  高通量测序  荒漠草原  多样性
英文关键词:Caragana korshinskii Kom. Nebkhas  soil fungi  high-throughput sequencing  desert steppe  diversity
基金项目:国家自然科学基金项目（31860158）；中央高校基本科研业务费北方民族大学高层次人才引进科研启动项目（2019KYQ001）；宁夏回族自治区科技创新领军人才项目（2021GKLRLX12）
作者单位
王子寅 北方民族大学生物科学与工程学院，宁夏 银川 750021；国家民委黄河流域农牧交错区生态保护重点实验室，宁夏 银川 750021；宁夏特殊生境微生物资源开发与利用重点实验室，宁夏 银川，750021 
刘秉儒 北方民族大学生物科学与工程学院，宁夏 银川 750021；国家民委黄河流域农牧交错区生态保护重点实验室，宁夏 银川 750021；宁夏特殊生境微生物资源开发与利用重点实验室，宁夏 银川，750021 
牟红霞 宁夏大学生态环境学院/西北退化生态系统恢复与重建教育部重点实验室，宁夏 银川 750021 
李子豪 北方民族大学生物科学与工程学院，宁夏 银川 750021；国家民委黄河流域农牧交错区生态保护重点实验室，宁夏 银川 750021；宁夏特殊生境微生物资源开发与利用重点实验室，宁夏 银川，750021 
摘要点击次数: 872
全文下载次数: 659
中文摘要:
      为了解荒漠草原柠条灌丛堆演化过程中环境微生物多样性变化与形成机理，以宁夏荒漠草原不同发育阶段的柠条灌丛堆为研究对象，采用高通量测序技术分析其土壤真菌的群落结构及多样性特征，探究发育阶段和土壤理化因子对土壤真菌群落结构和功能的影响因素。结果显示：获得的2662个真菌操作分类单元(Operational taxonomic unit，OTU)属于13门40纲524属，其中优势菌门为子囊菌门(Ascomycota，占77.48%~82.07%)、担子菌门(Basidiomycota，占7.42%~10.07%)以及被孢霉门(Mortierellomycota，占5.42%~10.78%)；优势菌纲有粪壳菌纲(Sordariomycetes，占27.10%~36.23%)、座囊菌纲(Dothideomycetes，占25.69%~35.30%)、散囊菌纲(Eurotiomycetes，占12.64%~16.33%)、伞菌纲(Agaricomycetes，占7.07%~7.57%)。土壤有机碳、速效磷、总氮、电导率在柠条灌丛堆不同发育阶段不同部位有显著性差异，而总磷、pH无明显差异。在柠条灌丛堆不同发育阶段Sobs、Chao 1无显著性差异，Ace指数有显著性差异，但丰富度指数在成熟期表现为最大值，而多样性指数(Shannon和Simpson 指数)无明显差异。土壤真菌共生关系大于竞争关系，且在柠条灌丛堆发育阶段土壤真菌功能群以腐生营养型真菌为主。土壤理化因子与土壤真菌群落的关系分析揭示速效磷和有机碳是土壤真菌群落结构的主要影响因子。
英文摘要:
      To understand the formation mechanism of the microbial diversity in the underground environment during the evolution of the Caragana korshinskii Kom. Nebkhas in the desert steppe, the southern desert grassland in Yanchi County of Ningxia was used as the research object, and the community structure of the soil fungus in the Caragana korshinskii Kom. Nebkhas was analyzed by high-throughput sequencing technology and diversity characteristics. The results showed that the 2662 obtained operational taxonomic units (OTU) belonged to 13 phyla, 40 classes and 524 genera. Of which the dominant phyla were Ascomycota (77.48%~82.07%), Basidiomycota (7.42%~10.07%) and Mortierellomycota (5.42%~10.78%). The dominant bacteria classes included Sordariomycetes (27.10%~36.23%), Dothideomycetes (25.69%~35.30%), Eurotiomycetes (12.64%~16.33%) and Agaricomycetes (7.07%~7.57%). The soil organic carbon, available phosphorus, total nitrogen, and eletrical conductivity of the soil had significant differences in different parts of the Caragana korshinskii Kom. Nebkhas at different development stages, but there was no significant difference in total phosphorus and pH. There were no significant differences in Sobs and Chao 1 at different developmental stages of Caragana korshinskii Kom. Nebkhas, and significant differences in Ace index, but the richness index showed a maximum at maturity while the diversity index (Shannon and Simpson indices) was not significantly different. By analyzing the relationship between soil physical and chemical factors and soil fungal community, it was found that availabe phosphorus and organic carbon were the main influencing factors of soil fungal community structure. The network analysis graph showed that the symbiotic relationship of soil fungi was greater than the competitive relationship, and the saprophytic trophic fungi were the main functional groups of soil fungi in the development stage of the Caragana korshinskii Kom. Nebkhas.
查看全文  查看/发表评论  下载PDF阅读器