| 张泽宇,曹红霞,裴书瑶,李曼宁.干旱胁迫下辣椒幼苗光合与荧光参数测定的最佳叶片[J].干旱地区农业研究,2022,40(4):33~40 |
| 干旱胁迫下辣椒幼苗光合与荧光参数测定的最佳叶片 |
| Optimum leaf based on photosynthesis and fluorescence parameters of pepper seedlings under drought stress |
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| DOI：10.7606/j.issn.1000-7601.2022.04.04 |
| 中文关键词: 干旱胁迫 辣椒 叶位 光合参数 荧光参数 |
| 英文关键词:drought stress pepper leaf position photosynthesis chlorophyll fluorescence |
| 基金项目:“十三五”国家重点研发计划（2016YFC0400204）；陕西省水利厅科技计划项目（2020slkj-08） |
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| 中文摘要: |
| 为明确干旱胁迫下不同叶位光合、荧光特性的变化规律，进而选择出典型叶片来准确测量干旱条件下植株光合、荧光参数，以实现干旱条件下植株光合能力的快速评价。研究通过盆栽试验对不同干旱处理下（充分供水（CK）：75%~85%田间持水量；中度胁迫（MS）：65%~75%田间持水量；重度胁迫（SS）:55%~65%田间持水量）辣椒幼苗不同叶位叶片的光合、荧光特性进行比较分析。结果表明：充分供水时，随叶位的增加，气体交换参数(净光合速率、气孔导度、蒸腾速率)、叶绿素含量、叶面积、叶绿素荧光参数(光化学淬灭系数、光系统Ⅱ最大光化学量子产量)均呈先增大后减小的趋势，在第3叶位达到最大值；在干旱条件时，各叶位的参数随水分胁迫程度的增加在1~3叶位逐渐减小，4~6叶位先增加后减小，并在第4叶位达到最大值(Ci、F0、Fv/Fm、NPQ除外);水分胁迫对辣椒叶片光合作用影响较大，MS与SS处理，第3叶位的Pn分别较CK下降14.05%和55.91%，第4叶位的Pn MS处理较CK增加25.20%，SS处理较CK降低15.42%；第3、4叶位是对辣椒幼苗生长具有主要贡献的叶片,但在水分胁迫条件下，第3叶位光合参数、叶面积、叶绿素含量及叶绿素荧光参数的变异系数较第4叶位更大，尤其对于Ci、Chlb、F0,在光合参数、叶绿素参数及叶绿素荧光参数中变幅最大，其第3叶位的变异系数分别较第4叶位增大了133.33%，180.00%及200.00%。第3叶位对于水分胁迫更加敏感，因此，在干旱胁迫实验中，宜选用第3叶位测量辣椒幼苗光合、荧光参数。 |
| 英文摘要: |
| The purpose of this study was to clarify the change rules of photosynthetic and fluorescence characteristics of different leaf positions under drought stress, and then select typical leaves to accurately measure photosynthetic and fluorescence parameters of plants under drought conditions, to achieve rapid evaluation of photosynthetic capacity of plants under drought conditions. The photosynthetic and fluorescence characteristics of pepper seedlings at different leaf positions under different drought treatments (Sufficient water supply (CK): 75%~85% field water capacity; Moderate stress (MS): 65%~75% field capacity; Severe stress (SS): 55%~65% field water capacity)were compared and analyzed by pot experiment. The results showed that, with the increase of the leaf position, the gas exchange parameters (net photosynthetic rate, transpiration rate, stomatal conductance), chlorophyll content, leaf area, chlorophyll fluorescence parameters (photochemical quenching coefficient，PSⅡmaximum quantum yield of photochemical reaction) first increased and then decreased and reached the maximum at 3rd leaf under sufficient water supplies. Under drought conditions, the parameters of each leaf position decreased gradually with the increase of water stress degree at 1st to 3rd leaf position, and the parameters increased first and then decreased at 4th to 6th leaf position, but all reached the maximum value at 4th leaf position(except Ci, F0, Fv/Fm, NPQ). Under MS and SS, the Pn of the third leaf position decreased by 14.05% and 55.91% compared with CK, respectively. Pn of the fourth leaf increased by 25.20% in MS compared with CK, and decreased by 15.42% in SS compared with CK. The third and fourth leaf positions were the main contributors to the growth of pepper seedlings. However, the variation coefficients of photosynthetic parameters, leaf area, chlorophyll concentration and chlorophyll fluorescence parameters at the third leaf position were larger than those at the fourth leaf position under water stress,especially for Ci, Chlb and F0, the variation of photosynthetic parameters, chlorophyll parameters and chlorophyll fluorescence parameters was the largest, and the coefficient of variation of the third leaf position was 133.33%, 180.00% and 200.00% higher than that of the fourth leaf position, respectively. The third leaf position was more sensitive to water stress. Therefore, the third leaf position should be selected to measure photosynthesis and fluorescence parameters of pepper seedlings in drought stress experiment. |
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