| 李丽华,郭颖彬,魏昕.4个禾本科植物NAC基因家族的系统进化及其在玉米中的干旱表达谱分析[J].干旱地区农业研究,2022,40(4):230~237 |
| 4个禾本科植物NAC基因家族的系统进化及其在玉米中的干旱表达谱分析 |
| Phylogenetic evolution of NAC gene families in four poaceae species and expression profile analysis of maize ZmNACs under drought stress |
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| DOI：10.7606/j.issn.1000-7601.2022.04.26 |
| 中文关键词: 禾本科植物 系统进化 NAC基因家族 干旱胁迫 玉米 基因表达 |
| 英文关键词:poaceae species phylogenetic analysis NAC genes drought stress maize gene expression |
| 基金项目:财政部和农业农村部：国家现代农业产业技术体系资助(CARS-02-05)；河南省基础前沿项目(162300410139)；河南省农业科学院自主创新专项基金(2020ZC06) |
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| 中文摘要: |
| 利用生物信息学方法，对4个禾本科物种玉米、高粱、二穗短柄草和水稻的NAC基因家族进行鉴定和系统进化分析。结果表明：4个禾本科物种共556个NAC 基因可以聚类为6个进化枝；物种内NAC转录因子有相似的理化性质，但在进化枝上又表现出差异，进化枝1、2、3和进化枝4、5、6分别表现出相似的相对分子质量，进化枝1、2、3、5表现出相似的等电点，进化枝4、6表现出相似的等电点；4个物种共鉴定出27个含跨膜结构域的NAC基因，并且大部分（88.9%）都位于进化枝4上；使用溯源法共鉴定出174个禾本科祖先基因，每个物种NAC基因丢失和保留都存在差异；基因复制分析发现，串联复制对禾本科NAC基因家族扩张贡献明显（20.3%，113/556），而玉米的大片段复制事件对玉米NAC基因家族扩张贡献明显（30.5%，50/164）；干旱胁迫下玉米转录表达分析鉴定出51个差异表达基因，且不同组织内的差异表达基因数目存在差异，叶和雌穗内的差异表达基因数目多于雄穗。 |
| 英文摘要: |
| In this research, we used bioinformatics methods to identify and analyze the NAC gene families in four poaceaes including Zea mays, Sorghum bicolor, Brachypodium distachyon and Oryza sativa. Evolutionary analysis showed that 556 NAC genes in these four poaceaes were clustered into six clades. NAC transcription factors in these four species had similar physicochemical properties but were different in clades. Clade 1, 2, 3 and clade 4, 5, 6 showed similar relative molecular weight, respectively, and clade 1, 2, 3, 5 showed similar isoelectric points, and so did in clade 4 and 6. A total of 27 NAC genes with transmembrane domains were identified in these four species, and most (88.9%) of them were in clade 4. A total of 174 poaceae ancestral genes were identified by the traceability method and differences existed in both the loss and expansion of NAC genes in each species. Duplication events analysis discovered that tandem duplication events contributed significantly to the expansion of NAC gene in poaceae (20.3%; 113/556), and segment duplication events in maize contributed significantly to the expansion of maize NAC genes (30.5%; 50/164). By transcriptional expression analysis, 51 differentially expressed genes were identified in maize under drought stress, and the number of differentially expressed genes in different tissues was different. The number of differentially expressed genes in leaves and female ears was more than that in male ears. This research provides references for further exploring the evolution of NAC genes in gramineous varieties and identifying functional NAC genes in maize. |
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