潘巧文,武军艳,马学才,王亚林,马骊,徐新宇,李鹏,姚彦林,徐芳,崔小茹,孙万仓.白菜型油菜BraSAUR基因家族鉴定及其表达分析[J].干旱地区农业研究,2022,40(5):1~12
白菜型油菜BraSAUR基因家族鉴定及其表达分析
Identification and expression analysis of BraSAUR gene family in rapeseed (Brassica rapa L.)
  
DOI:10.7606/j.issn.1000-7601.2022.05.01
中文关键词:  SAUR基因家族  白菜型油菜  鉴定  低温和干旱胁迫  表达模式
英文关键词:SAUR gene family  Brassica rapa L.  identification  low temperature and drought stress  expression pattern
基金项目:甘肃农业大学国家级大学生创新创业训练计划项目(202010733059);财政部和农业农村部:国家现代农业产业技术体系(CARS-12)
作者单位
潘巧文 甘肃农业大学农学院甘肃省油菜工程技术研究中心甘肃 兰州 730070 
武军艳 甘肃农业大学农学院甘肃省油菜工程技术研究中心甘肃 兰州 730070 
马学才 甘肃农业大学农学院甘肃省油菜工程技术研究中心甘肃 兰州 730070 
王亚林 甘肃农业大学农学院甘肃省油菜工程技术研究中心甘肃 兰州 730070 
马骊 省部共建干旱生境作物学国家重点实验室甘肃 兰州 730070 
徐新宇 甘肃农业大学农学院甘肃省油菜工程技术研究中心甘肃 兰州 730070 
李鹏 甘肃农业大学农学院甘肃省油菜工程技术研究中心甘肃 兰州 730070 
姚彦林 甘肃农业大学农学院甘肃省油菜工程技术研究中心甘肃 兰州 730070 
徐芳 甘肃农业大学农学院甘肃省油菜工程技术研究中心甘肃 兰州 730070 
崔小茹 甘肃省农业技术推广总站, 甘肃 兰州 730070 
孙万仓 甘肃农业大学农学院甘肃省油菜工程技术研究中心甘肃 兰州 730070 
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中文摘要:
      SAUR(Small auxin-up RNA)是生长素早期响应逆境胁迫的基因,本文研究白菜型油菜全基因组中SAUR家族的信息,分析了该家族成员的基本特征。以强抗寒性白菜型冬油菜品种陇油7号(L7)和弱抗寒性冬油菜品种陇Lenox(X)为试验材料,采用低温和干旱处理,应用荧光定量技术分析不同基因在不同品种中的表达特性,筛选差异表达基因,为研究生长素早期应答基因调控白菜型冬油菜生长点发育机理提供支撑。结果表明:白菜型油菜的BraSAUR基因共有142个,在10条染色体中不对称分布,主要分布在2、3号染色体上,基因长度较短,且大多数基因没有内含子。qRT-PCR结果表明,低温处理后,与CK相比,在两个品种的叶片中,Bra029452的表达量变化趋势不同,24 h时在L7中的表达量是X的24倍,在生长点中,Bra010501在L7中24 h时表达量达到CK的4倍,而在X中是先增加后降低的趋势。模拟干旱胁迫后,Bra029452在L7的叶片中的表达量均显著高于CK,在X中则是逐渐降低,在L7的生长点中1 h和24 h表达量均高于CK,但在X中则表现为先降低后升高。低温胁迫和干旱胁迫条件下,Bra029452基因表达量在强抗寒性油菜品种L7的叶片及生长点中均逐渐升高,可推断该基因同时参与白菜型冬油菜耐受低温、干旱的调控过程。
英文摘要:
      SAUR (Small auxin\|up RNA) is an early auxin response gene to stress. In this paper, we studied the information of SAUR family in the whole rapeseed genome (Brassica rapa L.) and analyzed the basic characteristics of the family members. Longyou 7 (L7) and Longlenox (X) were used as experimental materials with low temperature and drought stress treatments. The expression levels of different genes in different varieties were analyzed by fluorescence quantitative technique to provide support for the study of auxin early response gene regulation mechanism of winter rapeseed growing point. Results showed that a total of 142 SAUR genes were systematically identified from Brassica rapa, which were distributed on 10 chromosomes, mainly on chromosome 2 and 3 and divided into four groups according to the evolutionary tree relationship. The genes were short in length and most had no introns. The results of qRT-PCR showed that after low temperature treatment, the expression of Bra029452 in the leaves of two varieties had different trends compared with CK, and the expression of Bra010501 in the leaves of L7 was 24 times more than that of X at 24 h. The expression of Bra010501 in growing point of L7 was 4 times more than that of CK at 24 h, while in X was first increased and then decreased. Under low temperature stress and drought stress, Bra029452 gene increased gradually in the leaves and growing points of L7, suggesting that Bra029452 gene was involved in regulation of low temperature and drought tolerance of Brassica rapa.
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