徐锐,熊泽浩,朱旭东,周宾寒,侯泽豪,许嘉盛,罗旖柔,方正武.甜荞FeHSP83基因的克隆及其在逆境胁迫下的表达分析[J].干旱地区农业研究,2022,40(5):88~93
甜荞FeHSP83基因的克隆及其在逆境胁迫下的表达分析
Cloning and expression analysis of buckwheat FeHSP83 gene under drought and salt stress
  
DOI：10.7606/j.issn.1000-7601.2022.05.10
中文关键词:  甜荞  FeHSP83基因  克隆  逆境  表达模式
英文关键词:sweet buckwheat  FeHSP83 gene  clone  adversity  expression mode
基金项目:国家自然科学基金（31671755，31571736）
作者单位
徐锐 College of Agriculture, Yangtze University, Hubei Center for Collaborative Innovation of Grain Industry, Jingzhou, Hubei 434025, China 
熊泽浩 College of Agriculture, Yangtze University, Hubei Center for Collaborative Innovation of Grain Industry, Jingzhou, Hubei 434025, China 
朱旭东 College of Agriculture, Yangtze University, Hubei Center for Collaborative Innovation of Grain Industry, Jingzhou, Hubei 434025, China 
周宾寒 College of Agriculture, Yangtze University, Hubei Center for Collaborative Innovation of Grain Industry, Jingzhou, Hubei 434025, China 
侯泽豪 College of Agriculture, Yangtze University, Hubei Center for Collaborative Innovation of Grain Industry, Jingzhou, Hubei 434025, China 
许嘉盛 College of Agriculture, Yangtze University, Hubei Center for Collaborative Innovation of Grain Industry, Jingzhou, Hubei 434025, China 
罗旖柔 College of Agriculture, Yangtze University, Hubei Center for Collaborative Innovation of Grain Industry, Jingzhou, Hubei 434025, China 
方正武 College of Agriculture, Yangtze University, Hubei Center for Collaborative Innovation of Grain Industry, Jingzhou, Hubei 434025, China 
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中文摘要:
      为了探究甜荞FeHSP83基因在干旱和盐胁迫下的表达模式，本研究采用‘西农9976’为试验材料，通过15% PEG 6000溶液和0.1% NaCl溶液对荞麦幼苗进行胁迫处理。通过RT-PCR的方法，鉴定获得FeHSP83，该基因开放阅读框2 100 bp，编码704个氨基酸，预测其相对分子质量为80.97 kDa，理论等电点为4.96，具有较强亲水性。同源性分析结果显示，该基因与藜麦、甜菜亲缘关系较近，同源性达到97.06%。采用qRT-PCR的方法，对干旱和盐胁迫下FeHSP83基因在叶和根中的表达水平进行分析。结果显示，在干旱胁迫条件下，FeHSP83在甜荞根部的表达量持续上升，到48 h达到最大值，增加范围为1.03~10.20；叶的表达量呈先升后降的趋势；而在盐胁迫条件下，FeHSP83在植株叶部的响应较为明显，增加范围为1.07~6.24，在12 h时表达量达到最高；根部的表达量在3 h时迅速升高，随后缓慢下降。结果表明，FeHSP83基因在干旱和盐胁迫条件下能够快速响应胁迫，且在根和叶不同器官中的表达存在差异。预测FeHSP83基因在甜荞抗旱中起到一定作用。
英文摘要:
      This study used ‘Xinong 9976’ as the experimental material to explore the expression pattern of the FeHSP83 gene of sweet buckwheat under drought and salt stress. The buckwheat seedlings were subjected to stress treatment with 15% PEG 6000 solution and 0.1% NaCl solution. Through the RT-PCR method, FeHSP83 was identified. The gene had an open reading frame of 2 100 bp and encoded 704 amino acids. Its relative molecular mass was predicted to be 80.97 kDa, and the theoretical isoelectric point was 4.96. It had strong hydrophilicity. The result of homology analysis showed that the gene was closely related to quinoa and sugar beet, and the homology reached 97.06%. The qRT-PCR method was used to analyze the expression level of FeHSP83 gene in leaves and roots under drought and salt stress. The results showed that under drought stress, the expression of FeHSP83 in the roots of buckwheat continued to increase, reaching the maximum at 48 h, with an increase in the range of 1.03~10.20; the expression of leaves showed a trend of increasing first and then decreasing; while under salt stress conditions FeHSP83 had a more obvious response in the leaves of plants, and the increase range was 1.07~6.24, and the expression reached the highest at 12 h; the expression of roots increased rapidly at 3 h, and then slowly decreased. The results showed that FeHSP83 gene quickly responded to stress under drought and salt stress, and the expression of FeHSP83 gene in different organs of roots and leaves was different. Therefore, FeHSP83 gene played a certain role in drought resistance of sweet buckwheat.
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