| 王亚茹,杨向东,赵寒冬,牛陆,张原宇,杨春明,任伟.油莎豆种质资源苗期抗旱性鉴定与评价[J].干旱地区农业研究,2022,40(6):12~22 |
| 油莎豆种质资源苗期抗旱性鉴定与评价 |
| Characterization and assessment of drought resistance in tigernut germplasm resources at seedling stage |
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| DOI：10.7606/j.issn.1000-7601.2022.06.02 |
| 中文关键词: 油莎豆 种质评价 幼苗期 抗旱性 隶属函数 |
| 英文关键词:Cyperus esculeutus germplasm assessment seedling stage drought resistance membership function |
| 基金项目:国家重点研发计划课题（2019YFC0507601,2019YFC0507602） |
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| 中文摘要: |
| 油莎豆（Cyperus esculeutus L.）是一种新型生态经济型作物，开展油莎豆种质资源抗旱筛选与评价可为选育适沙抗旱新品种提供依据。本研究通过室内盆栽试验，测定39份油莎豆种质在正常灌溉和自然干旱胁迫条件下的17个表型和生理指标。采用相关性分析、主成分分析、隶属函数及聚类分析相结合的方法，对油莎豆苗期抗旱性进行综合评价。结果表明：干旱胁迫对油莎豆的叶宽、叶厚和根长具有显著影响（P＜0.05），对株高、分蘖数、地上干重、地下干重和总干重具有极显著影响（P＜0.01），不同种质材料间的变异系数介于0.148~0.584。5个主成分可以代表原17个指标74.174%的数据量。叶宽、枯叶率、根长、鲜重、干重、根冠比可以作为抗旱评价主要指标。聚类分析可将39份油莎豆种质由强到弱分为4个等级，1~4级分别含有2、10、16、11份种质。抗旱性度量值可作为油莎豆抗旱评价的适宜指标，基于此筛选出苗期抗旱性强的油莎豆种质资源为JYD-39、JYD-34和JYD-28。 |
| 英文摘要: |
| Tigernut (Cyperus esculeutus L.) is a multipurpose cultivated crop with high eco\|economic value. Characterization and assessment of drought resistance in tigernut germplasm resources can greatly facilitate the development of varieties adapted to the arid and semi\|arid sandy land widely distributed in China. In the present study, a total of 39 Tigernut accessions were tested for 17 phenotypical and physiological traits under the treatment of normal irrigation and drought stress in indoor pot\|planting experiment. Comprehensive assessment of drought resistance at seedling stage for these accessions was carried out by using correlation, principal component, membership function, and cluster analysis. The results showed that the effects of drought were significant on the width and thickness of leaf and root length (P＜0.05), and extremely significant on plant height, tiller number, dry weight of root and leaf, and total dry weight of plants (P＜0.01). The coefficient of variation among different germplasms ranged from 0.148 to 0.584. The results of principal component analysis using 17 phenotypical and physiological variables indicated that the first 5 principal components accounted for 74.174% of the total variation among these accessions. Leaf width, leaf yellowing rate, root length, fresh and dry weight, and crown root ratio were suitable as comprehensive criteria for evaluating drought resistance of Tigernut. Cluster analysis produced four major clusters based on their resistance to drought stress, with cluster I containing 2 accessions, cluster Ⅱ containing 10 accessions, cluster Ⅲ and cluster Ⅳ containing 16 and 11 accessions, respectively. Comprehensive evaluation value was an appropriate index for assessing drought resistance of Tigernut accessions. Based on the results of comprehensive assessment of drought resistance, 3 Tigernut accessions (JYD-39, JYD-34 and JYD-28) were identified as the germplasm with higher resistance to drought stress at seedling stage. |
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