董扬.240份糜子种质资源萌芽期耐冷性综合评价及筛选[J].干旱地区农业研究,2022,40(6):23~33
240份糜子种质资源萌芽期耐冷性综合评价及筛选
Comprehensive evaluation and screening of cold tolerance of 240 broomcorn millet germplasm resources at germination stage
  
DOI:10.7606/j.issn.1000-7601.2022.06.03
中文关键词:  糜子  萌芽期  耐冷性  综合评价
英文关键词:broomcom millet  bud stage  cold resistance  comprehensive evaluation
基金项目:黑龙江省农业科学院院级课题(2020YYYF035);国家农业现代产业技术体系建设专项(CARS\|06-14.5-B21);齐齐哈尔市科技计划创新激励项目(CNYGG-2020030);农业科技创新跨越工程杂粮杂豆科技创新专项(HNK2019CX05-06)
作者单位
董扬 黑龙江省农业科学院齐齐哈尔分院黑龙江 齐齐哈尔 161006 
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中文摘要:
      耐冷品种的选育是农业生产上解决低温冷害问题最直接有效的手段。以240份糜子资源为供试材料,研究了10℃低温胁迫(将25℃环境视为对照处理)对糜子萌芽期生长发育的影响,采用描述统计分析、相关线性分析、主成分分析和聚类分析,评估各糜子品种资源对冷胁迫的耐受性。结果表明:240份糜子资源在10℃冷胁迫下,萌发期各项指标平均值均低于对照处理,但不同品种的降幅存在显著差异,各指标变异系数的变化范围为20.59%~100.03%,表明参试糜子资源具有丰富的遗传多样性。相关分析表明:相对发芽势、相对发芽指数和相对发芽率之间呈极显著正相关,相对芽鲜重、相对活力指数和相对根鲜重之间呈极显著正相关,表明上述耐冷性状存在很大关联。主成分分析表明,前 3个主成分累计贡献率达到 87.22%,第1主成分主要与发芽势、发芽率、发芽指数有关,第2主成分主要与芽鲜重和根鲜重有关,第3主成分主要与根长和芽长有关。糜子资源耐冷性综合得分聚类分析结果显示,‘景泰疙瘩红’等22个品种为高度耐冷品种资源、‘张川麻糜子’等138个品种为耐冷品种资源、‘札达糜’等64个品种为冷敏感品种资源、‘PAN 21’等16个品种为高度冷敏感品种资源。
英文摘要:
      Breeding of cold\|resistant varieties is the most direct and effective means to solve the problem of cold damage in agricultural production. In this study, 240 millet resources were used as test materials and 25℃ environment was considered as the control treatment. The effects of low temperature stress at 10℃ on the growth and development of broomcorn millet at germination stage were studied. The tolerance of different broomcorn cultivars to cold stress was evaluated by descriptive statistical analysis, correlation linear analysis, principal component analysis and cluster analysis. The results showed that under cold stress at 10℃, the average values of each index at germination stage of 240 broomcorn millet resources were lower than those of the control group, but there were significant differences among different varieties. The variation coefficients of each index ranged from 20.59% to 100.03%, indicating that the broomcorn millet resources tested had rich genetic diversity. Correlation analysis showed that the relative germination potential, relative germination index and relative germination rate were significantly positively correlated, and the relative bud fresh weight, relative vigor index and relative root fresh weight were significantly positively correlated, indicating that these cold tolerance traits had a great degree of correlation. The results of principal component analysis showed that the cumulative contribution rate of the first three principal components reached 87.22%. The first principal component was mainly related to germination potential, germination rate and germination index. The second principal component was mainly related to fresh bud weight and root weight. The third principal component was mainly related to root and bud length. The cluster analysis of the comprehensive score of cold tolerance of the millet resources showed that Jingtai pimple red and other 22 varieties cultivars were highly cold tolerant, Zhangchuan sesame millet and other 138 varieties cultivars were cold tolerant, Zada millet and other 64 varieties cultivars were cold sensitive, and PAN 21 and other 16 varieties cultivars were highly cold sensitive.
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