低温胁迫下外源SA对酿酒葡萄幼苗抗氧化酶活性及内源SA含量的影响

王旺田; 李斌; 赵俊鹏; 王宝强; 张芮

王旺田,李斌,赵俊鹏,王宝强,张芮.低温胁迫下外源SA对酿酒葡萄幼苗抗氧化酶活性及内源SA含量的影响[J].干旱地区农业研究,2023,(1):78~85

低温胁迫下外源SA对酿酒葡萄幼苗抗氧化酶活性及内源SA含量的影响

Effects of exogenous SA on antioxidant enzyme activities and endogenous SA content in wine grapevine seedlings under low temperature stress

DOI：10.7606/j.issn.1000-7601.2023.01.10

中文关键词: 酿酒葡萄低温水杨酸抗氧化酶系幼苗

英文关键词:wine grapes low temperature salicylic acid antioxidant enzymes seedlings

基金项目:国家自然科学基金(31560552；52169007)；甘肃省农业生物技术研究及应用开发项目(GNSW-2014-12)；甘肃省科技计划项目(17JR5RA151)；甘肃省水利科学试验研究与技术推广计划项目（甘水建管发\[2021\]71号）

作者	单位
王旺田	甘肃省干旱生境作物学重点实验室，甘肃农业大学生命科学技术学院，甘肃兰州 730070
李斌	甘肃省科学院生物研究所，甘肃兰州 730099
赵俊鹏	庄浪县自然资源局，甘肃庄浪 744600
王宝强	甘肃省干旱生境作物学重点实验室，甘肃农业大学生命科学技术学院，甘肃兰州 730070
张芮	甘肃农业大学水利水电工程学院，甘肃兰州 730070

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中文摘要:

北方春季倒春寒限制了葡萄的栽种范围，严重影响了葡萄的产量和品质。利用室内模拟低温处理研究低温胁迫对葡萄幼苗膜氧化程度、抗氧化酶活性及内源SA含量的影响，结果发现,4℃、12℃低温处理相较于25℃而言，葡萄幼苗叶片MDA含量分别增加135.64%和68.38%，保护酶SOD、POD、CAT活性以及内源SA含量显著上升，4℃、12℃处理上升幅度分别为133.05%、53.33%、129.17%、46.22%和59.65%、26.67%、29.17%、24.65%。4℃与12℃处理相比，抗氧化物酶活性及内源SA含量均明显增加，SOD、POD、CAT活性以及内源SA含量分别增加45.97%、21.05%、77.42%及17.31%。恢复常温生长后低温处理幼苗叶片的抗氧化酶SOD、POD、CAT活性及内源SA含量没有明显降低，对葡萄幼苗组织的修复具有持续作用，且4℃处理的幼苗抗氧化酶SOD、POD、CAT活性分别是12℃处理的1.29倍、1.09倍、1.30倍。利用相关性及逐步回归分析法发现低温下叶片内源SA与MDA含量（R²=0.648）、SOD（R²=0.847）、POD（R²=0.436）、CAT（R²=0.584）活性呈正相关，SOD、CAT、POD活性与内源SA含量存在线性回归关系，保护酶活性受内源SA和低温信号的共同调控。外源SA和低温胁迫通过诱导内源SA的积累，显著增加SOD、POD和CAT的活性而提高葡萄对低温胁迫的耐受性。

英文摘要:

Spring inversions in north China limit the extent of grape planting and severely affect the yield and quality of grapes. The effects of low temperature stress on the degree of membrane oxidation, antioxidant enzyme activities and endogenous SA content of grape seedlings were investigated using indoor simulated low temperature treatments. The results showed that MDA content of grape seedling leaves increased significantly by 135.64% and 68.38% at 4℃ and 12℃, respectively, compared to 25℃, and the activities of protective enzymes SOD, POD, CAT and endogenous SA content increased significantly 133.05%, 53.33%, 129.17%, 46.22%, 59.65%, 26.67%, 29.17%, 24.65%. The antioxidant enzyme activities and endogenous SA content were increased significantly at 4℃ compared to 12℃, SOD, POD, CAT activities and endogenous SA contents increased by 45.97%, 21.05%, 77.42% and 17.31%, respectively. The antioxidant enzymes SOD, POD, CAT activities and endogenous SA content of low temperature treated seedlings leaves did not decrease significantly after restoration of normothermic growth and had a sustained effect on the repair of grape seedling tissue, and the antioxidant enzymes SOD, POD and CAT activities of the seedlings treated at 4℃ were 1.29, 1.09 and 1.30 times higher than those of the 12℃ treatment. The correlation and stepwise regression analysis found that endogenous SA was positively correlated with MDA content (R²=0.648), SOD (R²=0.847), POD (R²=0.436), and CAT (R²=0.584) activities under low temperature. There was a linear regression relationship between the activities of SOD, CAT and POD, and the content of endogenous SA. Antioxidant enzyme activities were regulated by both endogenous SA and low temperature signal. The antioxidant enzymes and endogenous SA played a sustained role in mitigating the damage of seedlings by low temperature stress. Exogenous SA and low temperature stress induced the accumulation of endogenous SA, which significantly increased the activities of SOD, POD, and CAT and improved the tolerance of grapes to low temperature stress.

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