| 王蕾,惠向娟,支慧,上官周平,李玮.一株淡紫紫孢菌对聚乙烯地膜降解能力的研究[J].干旱地区农业研究,2023,(4):217~225 |
| 一株淡紫紫孢菌对聚乙烯地膜降解能力的研究 |
| Degradation effects of a strain of purple violet spore on polyethylene film |
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| DOI：10.7606/j.issn.1000-7601.2023.04.23 |
| 中文关键词: 淡紫紫孢菌 聚乙烯地膜 降解能力 地膜颜色 |
| 英文关键词:purpureocillium lilacinum polyethylene film degradation ability film color |
| 基金项目:青海省自然科学基金面上项目（2022-ZJ-907）；青海省科技厅项目（2022-NK-KTP40） |
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| 中文摘要: |
| 为得到能够高效降解聚乙烯地膜的菌种资源，以青海大学农林科学院植保所提供的一株有降解聚乙烯潜力的菌株M2为研究对象，探究其对不同颜色（白色透明、黑色）聚乙烯地膜的降解能力。经形态学观察和ITS测序，确定菌株M2属于淡紫紫孢菌(Purpureocillium lilacinum)。以不接菌为对照，在30℃、150 r·min-1摇床培养条件下，分别于培养30、60 d各取样一次，利用高分辨场发射扫描电子显微镜、水接触仪和傅里叶变换红外光谱仪分析该菌株对不同颜色聚乙烯地膜的降解效果。扫描电镜观察发现，经过30 d的降解，两种地膜均出现微小的破碎现象；经过60 d的降解，两种地膜都出现明显的孔洞，表面粗糙度显著增加，黑色地膜被降解的程度更高。通过测定地膜失重率发现，M2对白色透明地膜降解率为7.27%，对黑色地膜的降解率可达8.45%；降解30 d后，黑色地膜疏水性较白色透明地膜显著降低5.51%，降解60 d后，黑色地膜疏水性较白色透明地膜显著降低21.35%。经傅里叶变换红外光谱仪分析，M2向白色透明膜片稳定的化学结构中引入醚键，黑色膜片则被引入羟基和醚键，黑色膜片的吸收峰强度大于白色地膜。研究初步认定，菌株M2对黑色聚乙烯地膜有较好的降解能力。 |
| 英文摘要: |
| To obtain strain resources that could efficiently degrade polyethylene mulch, a strain M2 with the potential to degrade polyethylene provided by Plant Protection Institute of Academy of Agricultural and Forestry Sciences, Qinghai University was used as the research object to explore its ability to degrade different colors (white transparent, black) of polyethylene film. The strain M2 was identified as Purpureocillium lilacinum by morphological observation and ITS sequencing. Under the condition of 30℃, 150 r·min-1 shaking culture, 30 d and 60 d sampling once respectively. The degradation effects of the strain on different color polyethylene film were analyzed by high resolution field emission scanning electron microscope, water contact instrument and Fourier transform infrared spectrometer. Scanning electron microscopy showed that after 30 days of degradation, both films showed slight fragmentation. After 60 days of degradation, both films showed obvious holes, the surface roughness increased significantly, and the black film was degraded more obviously. By measuring the weight loss rate of the film, the degradation rate of M2 on white transparent film reached 7.27%, and the degradation rate of black film reached 8.45%, indicating that the degradation ability of M2 to black film was greater than that of white transparent film. After 30 days of degradation, the hydrophobicity of black film was significantly lower than that of white transparent film by 5.51%. After 60 days of degradation, the hydrophobicity of black film was significantly lower than that of white transparent film by 21.35%. In addition, the surface hydrophobicity of both mulches decreased to varying degrees, and the hydrophobicity of black film was significantly reduced. Fourier transform infrared spectrometer analysis showed that M2 introduced ether bonds into the stable chemical structure of white transparent film, while black film was introduced by hydroxyl groups and ether bonds. The absorption peak intensity of black film was greater than that of white film. It was preliminarily determined that strain M2 had good degradation effects on black polyethylene film. |
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