| 赵永鲜,王凯彤,张宁,司怀军.马铃薯StTCP7基因在干旱和盐胁迫下的表达分析[J].干旱地区农业研究,2023,(6):27~38 |
| 马铃薯StTCP7基因在干旱和盐胁迫下的表达分析 |
| Expression analysis of potato StTCP7 gene under drought and salt stress |
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| DOI：10.7606/j.issn.1000-7601.2023.06.04 |
| 中文关键词: 马铃薯 StTCP7 基因克隆 干旱胁迫 盐胁迫 表达分析 |
| 英文关键词:potato StTCP7 gene cloning drought stress salt stress expression analysis |
| 基金项目:国家自然科学基金项目（31960444）；甘肃省科技重大专项计划项目（GZGG-2021-6）；科技部对发展中国家科技援助项目（KY201901015） |
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| 中文摘要: |
| 为了探究马铃薯（Solanum tuberosum L.）TCP7基因在干旱和盐胁迫下的表达模式，以耐旱品种‘Desiree’和干旱敏感品种‘Atlantic’为材料，通过自然干旱和200 mmol·L-1 NaCl溶液对盆栽苗进行胁迫处理。采用PCR法克隆得到StTCP7基因，CDS区长741 bp，启动子区含有响应干旱诱导的作用元件，蛋白序列与窄刀薯TCP7相似度最高，同源性为98.2%，共编码1个含246个氨基酸的蛋白质；该蛋白含有1个TCP保守结构域，属于碱性蛋白，定位于细胞核上。利用qRT-PCR方法，对干旱和盐胁迫下StTCP7基因在‘Desiree’和‘Atlantic’中的表达量进行分析发现，StTCP7基因表达存在器官差异和品种间差异，在‘Desiree’中的表达量表现为根（1.02）>茎（0.33）>叶（0.29），在‘Atlantic’中的表达量为叶（4.62）>茎（3.72）>根（1.00）。干旱胁迫下，StTCP7基因在‘Desiree’根和叶中的表达量随干旱胁迫程度的增加呈先上升后下降趋势，W3（土壤含水量为35%~45%）时达到峰值，其表达量分别为对照组的1.27倍和1.87倍；该基因在‘Atlantic’根部的表达量呈先上升后下降的趋势，W3（35%~45%）时达到峰值，其表达量为对照组的1.39倍，叶中表达量呈持续上升趋势，W4（15%~25%）时达到峰值，其表达量为对照组的2.52倍。盐处理下，随胁迫时间的增加，StTCP7基因在‘Desiree’根部的表达量呈先上升后下降的趋势，处理6 h时达到最高，为对照组的2.16倍；叶中表达量呈先上升后下降的趋势，处理3 h时达到最高，为对照组的4.54倍；该基因在‘Atlantic’根部的表达量呈现先上升后下降的趋势，胁迫3 h时达到最高，为对照组的2.12倍，叶中表达量呈上升趋势，在48 h时达到峰值，其表达量为对照组的4.04倍。综上所述，马铃薯StTCP7基因响应干旱与盐胁迫，且在根和叶不同器官中的表达存在差异，可为StTCP7基因在马铃薯非生物逆境响应中的功能研究提供基础。 |
| 英文摘要: |
| To explore the expression pattern of TCP7 gene in potato (Solanum tuberosum L.) under drought and salt stress, the pot seedlings of drought\|tolerant variety ‘Desiree’ and the drought\|sensitive variety ‘Atlantic’ used as the test material treating with the natural drought and 200 mmol·L-1 NaCl solution. The StTCP7 gene with CDS region length 741 bp, and promoter region containing action elements in response to drought induction was cloned by PCR, which encoded a protein containing 246 amino acids. the protein sequence of StTCP7 was the most similar to TCP7 (Solanum stenotomum) with homology of 98.2%, and this protein contains one conserved TCP domain and belongs to the alkaline protein, which is located on the nucleus. Using qRT-PCR, the analysis of StTCP7 gene expression in ‘Desiree’ and ‘Atlantic’ under drought and salt stress revealed that there were organ and inter\|breed differences. Expression in ‘Desiree’ was expressed as root (1.02)> stem (0.33)> leaf (0.29), and in ‘Atlantic’ as leaf (4.62)> stem (3.72)> root (1.00). Under the drought stress, the expression level of StTCP7 gene in ‘Desiree’ roots and leaves increased first and then decreased with the degree of drought stress, reaching the peak at W3 (soil moisture was 35%~45%), and its expression level was 1.27 and 1.87 times that of the control group respectively. The expression level of the gene in ‘Atlantic’ roots increased first and then decreased, reaching the peak at W3 (35%~45%), and its expression level was 1.39 times that of the control group. The expression level in leaves showed a continuous upward trend, reaching the peak at W4 (15%~25%), and its expression level was 2.52 times that of the control group. As for the salt treatment, the expression level of StTCP7 gene in ‘Desiree’ roots increased first and then decreased with increasing salt stress time, and the expression level reached the highest at 6 h, which was 2.16 times that of the control group. The expression level in leaves increased first and then decreased, reaching the peak at 3 h, and its expression level was 4.54 times that of the control group. The expression level of the gene in ‘Atlantic’ roots increased first and then decreased, reaching the highest at 3 h, which was 2.12 times that of the control group. The expression level in leaves showed an upward trend, reaching the peak at 48 h, and its expression level was 4.04 times that of the control group. To sum up, StTCP7 gene responded to drought and salt stress, and its expression in different organs of roots and leaves was different, which would provide a basis for functional research of potato StTCP7 gene in abiotic stress response. |
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