80份紫花苜蓿引进品种萌发期耐盐性评价

王宁; 万畅; 高山; 闫程铭; 李忠和; 王俊乔; 任伟; 王志锋

王宁,万畅,高山,闫程铭,李忠和,王俊乔,任伟,王志锋.80份紫花苜蓿引进品种萌发期耐盐性评价[J].干旱地区农业研究,2024,(2):17~25

80份紫花苜蓿引进品种萌发期耐盐性评价

Evaluation of salt tolerance of 80 introduced alfalfa varieties during germination

DOI：10.7606/j.issn.1000-7601.2024.02.03

中文关键词: 紫花苜蓿引进品种萌发期耐盐性评价

英文关键词:alfalfa introduced variety germination period salt tolerance evaluation

基金项目:吉林省科技发展计划人才项目（20230508015RC）;现代农业产业技术体系（CARS-34）

作者	单位
王宁	吉林师范大学生命科学学院，吉林四平 136000；吉林省农业科学院（中国农业科技东北创新中心），吉林长春 130124
万畅	吉林省农业科学院（中国农业科技东北创新中心），吉林长春 130124
高山	吉林省农业科学院（中国农业科技东北创新中心），吉林长春 130124
闫程铭	吉林省农业科学院（中国农业科技东北创新中心），吉林长春 130124
李忠和	吉林省农业科学院（中国农业科技东北创新中心），吉林长春 130124
王俊乔	吉林省农业科学院（中国农业科技东北创新中心），吉林长春 130124
任伟	吉林省农业科学院（中国农业科技东北创新中心），吉林长春 130124
王志锋	吉林省农业科学院（中国农业科技东北创新中心），吉林长春 130124

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中文摘要:

通过室内萌发试验，以80份引进紫花苜蓿主推品种为材料，研究1.2% NaCl处理对紫花苜蓿萌发期根长、芽长、发芽指数、发芽率、发芽势、活力指数及根芽比的影响。采用主成分分析、隶属函数及聚类分析相结合的方法，对紫花苜蓿萌发期的耐盐性开展综合性评价。结果表明：盐胁迫极显著影响紫花苜蓿的发芽势、发芽率和发芽指数（P<0.01），不同品种间的变异系数介于0.008～0.723。两个主成分的累计贡献率为73.922%。发芽势与根芽比可作为紫花苜蓿萌发期耐盐性筛选的主要评价指标。聚类分析结果将80份紫花苜蓿品种划分为5个级别，其中强耐盐级别包括9份品种；较强耐盐级别包括14份品种；中等耐盐级别包括43份品种；弱耐盐级别包括13份品种；最弱耐盐级别包括1份品种。在此基础上，筛选出萌发期强耐盐级别紫花苜蓿品种为：WL168HQ、游客、天马、迪特、巨能801和巨能995。

英文摘要:

In this study, the effects of salt stress on root length, bud length, germination index, germination rate, germination energy, vitality index and root\|bud ratio of 80 introduced alfalfa varieties treated with 1.2% NaCl were studied through laboratory germination test. A comprehensive evaluation of the salt tolerance of alfalfa during its germination period was conducted using a combination of principal component analysis, membership function, and cluster analysis. The results showed that salt stress significantly affected the germination energy, germination rate, and germination index of alfalfa (P<0.01), and the coefficient of variation between different varieties ranged from 0.008 to 0.723. The cumulative contribution rate of the two principal components was 73.922%. The germination energy and root\|bud ratio were used as the main salt tolerance indicators for salt tolerance screening during the germination period of alfalfa. The clustering analysis results divided 80 alfalfa varieties into 5 levels, including 9 varieties with strongest salt tolerance, 14 varieties with strong salt tolerance, 43 varieties with moderate salt tolerance, 13 varieties with weak salt tolerance, and 1 variety with the weakest salt tolerance. On this basis, varieties of alfalfa with strong salt tolerance during the germination period were selected as follows: WL168HQ, Tourists, Tianma, Dite, Juneng 801, and Juneng 995.

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