| 刘晓晨,王树凤,寿松涛,张建锋.复合型抗盐剂对NaCl胁迫下小麦种子萌发和生长的影响[J].干旱地区农业研究,2024,(2):199~209 |
| 复合型抗盐剂对NaCl胁迫下小麦种子萌发和生长的影响 |
| Effects of compound anti\|salt agent on seed germination and growth of wheat under NaCl stress |
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| DOI：10.7606/j.issn.1000-7601.2024.02.22 |
| 中文关键词: 复合抗盐剂 小麦 盐胁迫 种子萌发 抗性生理 |
| 英文关键词:compound anti\|salt agent wheat salt stress seed germination resistance physiology |
| 基金项目:浙江省重点研发计划项目(2021C02038) |
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| 摘要点击次数: 136 |
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| 中文摘要: |
| 将KNO3、CaCl2、DA-6、KH2PO4、FeSO4·7H2O、MgSO4·7H2O和蔗糖7种物质组合配制复合型抗盐剂，以‘济麦22’为研究材料，采用水培法，探讨了不同浓度(0.2%、0.4%和0.6%)盐(NaCl)胁迫下，施加不同浓度(10%、20%和30%)抗盐剂对种子萌发及后续幼苗生长的影响，并提出了适合小麦的抗盐剂配方。结果表明: (1)30%、20%、10%抗盐剂分别对0.2%、0.4%、0.6%NaCl胁迫下小麦种子萌发的促进效果最好，发芽率分别提高了6.4%、12.0%、26.5%。(2)0.6% NaCl胁迫下添加抗盐剂，根干质量显著增加了29.7%~35.9%；在抗盐剂作用下，光合速率分别提高了67.5%~90.0%(0.2% NaCl胁迫)、13.3%~51.5%(0.4% NaCl胁迫)和0.4%~25.5%(0.6% NaCl胁迫)，且30%抗盐剂显著增强了0.2% NaCl胁迫下小麦的光合作用，增幅达90.0%。添加抗盐剂使小麦的光合色素含量升高，其中叶绿素a在0.2%和0.4% NaCl胁迫下分别升高了22.5%~39.0%和41.7%~43.2%，叶绿素b分别升高了22.1%~31.0%和14.7%~22.5%，类胡萝卜素分别升高了12.2%~28.6%和24.4%~32.3%。此外，其他各项生理指标也有相应改善。(3)主成分分析结果显示，盐胁迫下30%抗盐剂处理后小麦的得分均为最高，为最适浓度配比。 |
| 英文摘要: |
| Seven substances, including KNO3、CaCl2、DA-6、KH2PO4、FeSO4·7H2O、MgSO4·7H2O, and sucrose were used to formulate compound anti\|salt agent. Using ‘Jimai 22’ as test material, the water cultivaton method was employed to investigate the effects of different concentrations of anti\|salt agent (10%, 20% and 30%) on seed germination and subsequent seedling growth under different salt (NaCl) stress (0.2%, 0.4% and 0.6%), and consequently the suitable formulation of anti\|salt agent for wheat was proposed. The results showed that: (1) 30%, 20% and 10% anti\|salt agent had the best effect in promoting wheat seed germination under 0.2%, 0.4% and 0.6% salt stress, and the germination rate increased by 6.4%, 12.0% and 26.5%, respectively. (2) Adding anti\|salt agents at a salt concentration of 0.6%, root dry mass significantly increased by 29.7% to 35.9%. Photosynthetic rate was increased after the addition of anti\|salt agent by 67.5%~90.0% (salt concentration 0.2%), 13.3%~51.5% (salt concentration 0.4%) and 0.4%~25.5% (salt concentration 0.6%), respectively. The 30% anti\|salt agent significantly enhanced photosynthesis at a salt concentration of 0.2%, with an increase of 90.0%. The photosynthetic pigment content of wheat was increased, with Chla content increased by 22.5%~39.0% (salt concentration 0.2%), 41.7% to 43.2% (salt concentration 0.4%); and Chlb content increased by 22.1%~31.0% (salt concentration 0.2%), 14.7%~22.5% (salt concentration 0.4%); and carotenoid content increased by 12.2% to 28.6% (salt concentration 0.2%) and 24.4% to 32.3% (salt concentration 0.4%), respectively. In addition, other physiological indicators also had corresponding improvement. (3) Based on the principal component analysis, the results showed that wheat treated with 30% anti\|salt agent had the highest score, so 30% anti\|salt agent was the most suitable concentration ratio. |
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