赵少迪,胡传伟,张玉莲,吕娜,曹利萍,马名川,刘璋,刘龙龙,张丽君.苦荞种质资源萌发期抗旱性综合评价[J].干旱地区农业研究,2024,(3):13~21
苦荞种质资源萌发期抗旱性综合评价
Comprehensive evaluation of drought resistance of Tartary buckwheat (Fagopyrum tataricum) germplasm resources at germination stage
  
DOI:10.7606/j.issn.1000-7601.2024.03.02
中文关键词:  苦荞  种质  PEG胁迫  萌发期  抗旱性评价
英文关键词:Tartary buckwheat  germplasm  PEG stress  germination period  drought resistance evaluation
基金项目:中央引导地方科技发展资金项目(YDZJSX2021A031);山西省基础研究计划项目(202103021224159);山西省现代产业技术体系建设(2023CYJSTX03-11);财政部和农业农村部:国家现代农业产业技术体系(CARS-07-A-2);山西农业大学博士项目(2021BQ38);山西省高等学校科技创新项目(2022L092)
作者单位
赵少迪 山西农业大学农业基因资源研究中心山西 太原 030031山西农业大学农学院山西 晋中 030801 
胡传伟 山西农业大学农业基因资源研究中心山西 太原 030031山西农业大学农学院山西 晋中 030801 
张玉莲 山西农业大学经济作物研究所山西 太原 030031 
吕娜 山西农业大学农业基因资源研究中心山西 太原 030031山西农业大学农学院山西 晋中 030801 
曹利萍 山西农业大学农业基因资源研究中心山西 太原 030031 
马名川 山西农业大学农业基因资源研究中心山西 太原 030031 
刘璋 山西农业大学农业基因资源研究中心山西 太原 030031 
刘龙龙 山西农业大学农业基因资源研究中心山西 太原 030031 
张丽君 山西农业大学农业基因资源研究中心山西 太原 030031 
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中文摘要:
      以PEG-6000为渗透剂对110份苦荞种质资源进行模拟干旱处理,研究了苦荞种子在0、5%、10%、15%、20%的PEG-6000溶液胁迫下的萌发情况,分析了种子的相对发芽势、相对发芽率、相对萌发指数、相对胚根长和相对胚根粗和萌发耐旱指数的变化。结果表明:15% PEG-6000可以作为苦荞种子萌发期耐旱性鉴定的适宜浓度;通过主成分分析法确定了相对发芽势、相对发芽率、相对萌发指数为关键指标,利用隶属函数结合权重分析法得到了萌发期苦荞种质资源耐旱性的综合评价值D。根据D值排序,筛选鉴定到耐旱型5份、较耐旱型28份、中间型48份、敏感型29份。通过聚类分析将110份苦荞种质资源划分为5个亚群,亚群Ⅰ是一类耐旱能力较强的亚群,亚群Ⅱ和亚群Ⅲ为中度耐旱亚群,亚群Ⅳ和亚群Ⅴ为典型的旱敏感亚群。
英文摘要:
      110 Tartary buckwheat germplasm resources were treated with PEG-6000 as an osmotic agent to simulate drought treatment. The germination of Tartary buckwheat seeds under 0, 5%, 10%, 15% and 20% PEG-6000 solution stress were studied. The changes of relative germination potential, relative germination rate, relative germination index, relative radicle length and relative radicle diameter and germination drought tolerance index of seeds were analyzed. The results showed that 15% PEG-6000 was the suitable concentration for the identification of drought tolerance of Tartary buckwheat seeds during germination. The relative germination potential, relative germination rate and relative germination index were determined as the key indexes by principal component analysis. The comprehensive evaluation value D of drought tolerance of Tartary buckwheat germplasm resources at germination stage was obtained by membership function combined with weight analysis. According to the D value, 5 drought\|tolerant types, 28 drought\|tolerant types, 48 intermediate types and 29 sensitive types were screened and identified. 110 Tartary buckwheat germplasm resources were divided into 5 subgroups by cluster analysis. Subgroup I was a subgroup with strong drought tolerance, subgroup II and subgroup III were moderate drought tolerance subgroups, and subgroup IV and subgroup V were typical drought sensitive subgroups.
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