刘婕,骆文琪,明立伟,马璐,齐翔鲲,付健,杨克军,王玉凤.干旱胁迫下两种芽胞杆菌对玉米幼苗促生作用研究[J].干旱地区农业研究,2024,(3):35~43
干旱胁迫下两种芽胞杆菌对玉米幼苗促生作用研究
Growth promotion effects of two Bacillus species on maize seedlings under drought stress
  
DOI:10.7606/j.issn.1000-7601.2024.03.04
中文关键词:  芽胞杆菌  浸种  玉米幼苗  干旱胁迫  促生作用
英文关键词:Bacillus  seed soaking  corn seedlings  drought stress  growth promotion
基金项目:东北盐碱植被恢复与重建教育部重点实验室(东北林业大学)开放基金(0924210202);黑龙江省外向型农业产业技术体系项目(2023);黑龙江八一农垦大学试验示范基地项目-鲜食玉米绿色丰产增效栽培技术集成与示范(2023)
作者单位
刘婕 黑龙江八一农垦大学农学院黑龙江 大庆 163319 
骆文琪 黑龙江八一农垦大学农学院黑龙江 大庆 163319 
明立伟 黑龙江八一农垦大学农学院黑龙江 大庆 163319 黑龙江省现代农业栽培技术与作物种质改良重点实验室黑龙江 大庆 163319 
马璐 黑龙江八一农垦大学农学院黑龙江 大庆 163319 
齐翔鲲 黑龙江八一农垦大学农学院黑龙江 大庆 163319 
付健 黑龙江八一农垦大学农学院黑龙江 大庆 163319 黑龙江省现代农业栽培技术与作物种质改良重点实验室黑龙江 大庆 163319 
杨克军 黑龙江八一农垦大学农学院黑龙江 大庆 163319 黑龙江省现代农业栽培技术与作物种质改良重点实验室黑龙江 大庆 163319 
王玉凤 黑龙江八一农垦大学农学院黑龙江 大庆 163319 黑龙江省现代农业栽培技术与作物种质改良重点实验室黑龙江 大庆 163319 东北林业大学东北盐碱植被恢复与重建教育部重点实验室黑龙江 大庆 163319 
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中文摘要:
      供试玉米品种为‘先玉335’(XY335),供试菌株为NECC11322(Bacillus subtilis)枯草芽胞杆菌、NECC11324(B. megaterium)巨大芽胞杆菌。试验设4组处理:Con(无菌水浸种,干旱胁迫),C1(无菌水浸种,正常供水),C2(NECC11322菌液浸种,干旱胁迫),C3(NECC11324菌液浸种,干旱胁迫),测定了浸种后盆栽玉米幼苗的生长状况、抗性生理指标及植株的N、P、K含量。结果表明:在干旱胁迫下接种NECC11322、NECC11324后,玉米幼苗叶片及根系SOD、POD、CAT、APX活性较Con均不同程度升高,其中接种NECC11322根系POD活性增长最为显著,较Con增长67.78%;植株叶片及根系可溶性蛋白含量、可溶性糖含量较Con均呈上升趋势,其中接种NECC11322叶片可溶性糖含量增长最为显著,较Con增长152.10%;与Con相比,植株叶片及根系全氮、全磷、全钾养分含量均有所提升,接种NECC11322后叶片全氮含量升高最为显著,较Con增长88.47%;干旱胁迫下接种两种芽胞杆菌后,植株叶片及根系丙二醛含量较Con均显著降低,接种NECC11322[JP2]后根系降幅最为显著,较Con降低51.03%。综上可知,干旱胁迫下接种两种芽胞杆菌均可降低干旱胁迫对玉米幼苗生长的抑制,其中NECC11322菌株更具有抗旱性。通过提高保护酶活性、可溶性蛋白、可溶性糖含量、叶绿素含量以及植株养分含量提高玉米幼苗抗旱性,促进玉米幼苗生长。
英文摘要:
      This experiment used corn variety XY335 and bacterial strains NECC11322 (Bacillus subtilis) and NECC11324 (B. megaterium). Four treatments were established: Con (sterile water soaking, drought stress), C1 (sterile water soaking, normal watering), C2 (NECC11322 bacterial liquid soaking, drought stress), and C3 (NECC11324 bacterial liquid soaking, drought stress). The growth status, resistance physiological indicators, and N, P, and K content of potted maize seedlings after soaking were measured. The results showed that under drought stress, the activities of SOD, POD, CAT, and APX in the leaves and roots of maize seedlings increased to varying degrees compared to Con after inoculation with NECC11322 and NECC11324. Among them, the POD activity in the roots of maize seedlings increased most significantly after inoculation with NECC11322, with an increase of 67.78% compared to Con. The content of soluble protein and soluble sugar in plant leaves and roots showed an increasing trend compared to Con, with the soluble sugar content in leaves inoculated with NECC11322 showing the most significant increase, increasing by 152.10% compared to Con. Compared with Con, the total nitrogen, total phosphorus, and total potassium nutrient content in plant leaves and roots were all increased. After inoculation with NECC11322, the total nitrogen content in leaves increased most significantly, with an increase of 88.47% compared to Con. Under drought stress, after inoculation with two types of Bacillus, the content of malondialdehyde in plant leaves and roots significantly decreased compared to Con. After inoculation with NECC11322, the root system showed the most significant decrease, which was 51.03% lower than Con. In summary, inoculating two types of Bacillus subtilis under drought stress reduced the inhibition of drought stress on the growth of maize seedlings, with NECC11322 strain being more drought resistant. By increasing the activity of protective enzymes, soluble protein, soluble sugar content, chlorophyll content, and plant nutrient content, the drought resistance of maize seedlings were improved, promoting their growth.
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