| 党林学,董小云,郑国强,王莹,王小霞,巩永杰,魏家萍,崔俊美,武泽峰,方彦,赵彩霞,刘自刚.干旱环境下油菜籽粒芥酸含量的QTL定位分析[J].干旱地区农业研究,2024,(3):53~59 |
| 干旱环境下油菜籽粒芥酸含量的QTL定位分析 |
| QTL mapping analysis of erucic acid content in rapeseed grains under drought conditions |
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| DOI：10.7606/j.issn.1000-7601.2024.03.06 |
| 中文关键词: 冬油菜 干旱环境 芥酸 QTL定位 |
| 英文关键词:winter rapseed arid environment erucic acid QTL mapping |
| 基金项目:甘肃省科技重大专项（22ZD6NA009）；国家自然科学基金项目（32360520）；甘肃省重点人才项目（GSRC-2023-1-2）；甘肃省自然科学基金重点项目（23JRRA1408）；省部共建干旱生境作物学国家重点实验室主任基金（GSCS-2021-Z01）；甘肃省教育厅产业支撑计划项目（2021CYZC-46）；中央引导地方科技专项资金（ZCYD2020-2-3） |
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| 中文摘要: |
| 为探讨干旱环境下冬油菜籽粒品质（脂肪酸含量和硫苷）分子遗传机理，以17NTS57（高芥酸）×CY12PXW-6-1（低芥酸）的衍生后代F1、F2、F2∶3等群体为材料，结合天水和定西试点籽粒品质表型，进行干旱环境下籽粒芥酸的遗传定位及选择标记开发。结果表明：利用F2∶3群体在两个试点共检测到油菜芥酸含量QTL位点7个，其中天水试点检测到4个，定西试点检测到3个。天水试点检测到2个主效QTL，即[STBX]qEATC08.2、qEATC09[STBZ]，分别分布在C08、C09染色体，其表型贡献率在20%以上；[STBX]qEATA10（天水）和qEADA10[STBZ]（定西）被定位到A10染色体相同区间，在两个试点的表型贡献分别为10.42%和12.70%；[STBX]qEATC09和qEADC09[STBZ]被定位在C09染色体相同区间，两个试点的表型贡献率分别为20.00%和19.34%，为共定位主效QTL。3个QTL（[STBX]qEATC08.1（天水）、qEATC08.2（天水）、qEADC07（定西）[STBZ]）仅各在一个试点被检测到，其中2个微效修饰位点（[STBX]qEATC08.1和qEADC07[STBZ]）具有正向加性效应。在A10和C09染色体上定位的[STBX]qEA.A10和qEA.C09[STBZ]的主效QTL，这些标记可用于干旱条件下油菜籽粒芥酸含量改良的分子标记辅助选择。 |
| 英文摘要: |
| To explore molecular genetic mechanism of the erucic acid accumulation in winter rapeseed grains under drought conditions, F1, F2 and F2∶3 populations derived from 17NTS57 (high erucic acid) × CY12PXW-6-1 (low erucic acid) were used as materials to analyze the genetic localization of erucic acid in this study. The results showed that a total of 7 QTLs for erucic acid content were detected using the F2∶3 population in two conditions, including 4 QTLs for erucic acid content in Tianshui condition and 3 QTLs for erucic acid content in Dingxi condition. Two main QTLs were detected in the Tianshui condition, namely [STBX]qEATC08.2[STBZ] and [STBX]qEATC09[STBZ], distributed on chromosomes C08 and C09, with a phenotypic contribution rate of over 20%. [STBX]qEATA10 (Tianshui)[STBZ] and [STBX]qEADA10[STBZ] (Dingxi) were in the same interval of A10 chromosome, [JP2]and their phenotypic contributions in both environments were 10.42% and 12.70%, respectively. [STBX]qEATC09[STBZ] and [STBX]qEADC09[STBZ] [JP2]were located in the same interval of the C09 chromosome, with phenotypic contribution rates of 20.0% and 19.34% in the two conditions, respectively, making them the main QTLs for co\|localization. Three QTLs ([STBX]qEATC08.1(Tianshui), qEATC08.2(Tianshui), qEADC07[STBZ](Dingxi)) were detected only in one condition, among which two minor modification sites ([STBX]qEATC08.1[STBZ] and [STBX]qEADC07[STBZ]) had a positive additive effect. The major QTLs of qEA.A[STBX]10 and qEA.C09[STBZ] mapping on chromosomes A10 and C09 can be used for molecular marker\|assisted selection for the improvement of erucic acid content in rapeseed under drought conditions. |
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