张春梅,祁世明,闫芳,赵刚,宋海,张喜峰,陈叶.基于转录组测序解析干旱胁迫对祁连山黄参基因表达的调控[J].干旱地区农业研究,2024,(3):68~79
基于转录组测序解析干旱胁迫对祁连山黄参基因表达的调控
Sequencing and analysis of transcriptome on regulating gene expression of Sphallerocarpus gracilis in Qilian Mountains under drought stress
  
DOI:10.7606/j.issn.1000-7601.2024.03.08
中文关键词:  黄参  干旱胁迫  转录组学测序  差异基因
英文关键词:Sphallerocarpus gracilis  drought stress  transcriptomics sequencing  differentially expressed genes
基金项目:国家自然科学基金项目(32160745);甘肃省自然科学基金项目(22JR5RG566)
作者单位
张春梅 河西学院农业与生态工程学院甘肃 张掖 734000
甘肃省河西走廊特色资源利用重点实验室甘肃 张掖 734000 
祁世明 河西学院农业与生态工程学院甘肃 张掖 734000
甘肃省河西走廊特色资源利用重点实验室甘肃 张掖 734000 
闫芳 甘肃省河西走廊特色资源利用重点实验室甘肃 张掖 734000
河西学院生态与绿洲农业研究院甘肃 张掖 734000 
赵刚 河西学院农业与生态工程学院甘肃 张掖 734000
甘肃省河西走廊特色资源利用重点实验室甘肃 张掖 734000 
宋海 甘肃省河西走廊特色资源利用重点实验室甘肃 张掖 734000 
张喜峰 河西学院农业与生态工程学院甘肃 张掖 734000
甘肃省河西走廊特色资源利用重点实验室甘肃 张掖 734000 
陈叶 河西学院农业与生态工程学院甘肃 张掖 734000
甘肃省河西走廊特色资源利用重点实验室甘肃 张掖 734000 
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中文摘要:
      以栽培2个月的黄参为试材,设置对照(土壤相对含水量70%~80%)和适度干旱胁迫(土壤相对含水量55%~60%)处理,利用高通量转录组测序BGISEQ-500平台,对测序结果进行基因功能注释、差异表达基因(DEGs, differentially expressed genes)筛选。结果表明:(1)获得的68193条Unigene中,分别有34230(50.20%)、34170(50.11%)、31727(46.53%)、27701(40.62%)、27092(39.73%)和22793(33.42%)个Unigene分别被分配到NCBI非冗余蛋白(NR)、eggNOG(基因的进化谱系, Evolutionary genealogy of genes: Non\|supervised Orthologous Groups)、基因本体(Gene ontology,GO)、Pfam (Protein family)、SwissProt (Reviewed protein sequence database)和KEGG (Kyoto encyelopedia of genes and genomes)六大功能数据库。(2)DEGs分析显示,黄参块状根和叶中分别有10674个和13402个DEGs;GO富集结果表明,根和叶中的DEGs功能部位中的分布基本一致,主要富集在生物过程、DNA的复制和翻译调控、氧化还原过程、蛋白质磷酸化、防御响应等;KEGG富集分析表明,根中DEGs显著富集在苯丙烷类生物合成、半乳糖代谢、半胱氨酸和甲硫氨酸代谢、淀粉和蔗糖代谢、植物-病原菌相互作用、植物激素信号转导等途径,叶中DEGs则主要富集在半乳糖代谢、淀粉和蔗糖代谢、苯丙烷类生物合成、戊糖、葡萄糖醛酸转换、植物激素信号转导等途径,说明淀粉和蔗糖代谢、半乳糖代谢、苯丙烷类生物合成途径、植物激素信号转导途径在黄参应对干旱胁迫中起重要作用。干旱胁迫影响黄参不同器官中差异基因的表达,为解析黄参耐受干旱的生物学途径、黄参药效成分的生物合成和分子机制提供了理论依据。
英文摘要:
      This study used the roots and leaves of 2-month-old S.gracilis from moderate drought stressed group and control group (the relative water content in soil was 55%~60% and 70%~80%, respectively) as the test material. The transcriptome sequencing analysis was carried out by using BGISEQ-500. After obtaining transcriptome data, gene function annotation, and differentially expressed genes (DEGs) screening of S.gracilis leaf and root were performed. The results showed: (1) A total of 34230 (50.20%), 34170 (50.11%), 31727 (46.53%), 27701 (40.62%), 27092 (39.73%), and 22793 (33.42%) were annotated by non\|redundant protein sequence database (NR), eggNOG, gene ontology (GO), Pfam databases, SwissProt and Kyoto Encyclopedia of genes and genomes (KEGG)respectively. (2) There were 10674 and 13402 DEGs in roots and leaves, respectively. At the same time, GO enrichment analysis showed that DEGs of root and leaf were distributed the same in GO functional annotations, mainly focusing on biological process, regulation of transcription, DNA-templated, protein phosphorylation, oxidation\|reduction process and defense response. Moreover, the KEGG pathway analysis showed that DEGs in roots were significantly enriched in phenylpropanoid biosynthesis, galactose metabolism, cysteine and methionine metabolism, starch and sucrose metabolism, plant\|pathogen interaction and plant hormone signal transduction. The DEGs in leaves were mainly concentrated in galactose metabolism, starch and sucrose metabolism, phenylpropanoid biosynthesis, pentose and glucuronate interconversions and plant hormone signal transduction, indicating that drought stress mainly affected starch and sucrose metabolism, galactose metabolism, phenylpropanoid biosynthesis pathways and plant hormone signal transduction in the roots and leaves of S.gracilis as well as these biological pathways played an important role in the response under drought stress. The high\|throughput transcriptome sequencing revealed the regulatory characteristics of moderate drought stress on gene expression in different organs of S.gracilis. In short, drought stress affected the expression of differential genes in different organs. The study can provide a theoretical basis for analyzing the biological pathway of tolerance of S.gracilis, biosynthesis and molecular mechanism of effective components under drought stress.
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