| 陆岸芷,张乐毅,王平平,陈新颖,杜姗姗,李先锋,王辉,张强,艾绥龙,张立新.半干旱区2种雪茄烟叶发酵后差异代谢物分析[J].干旱地区农业研究,2024,(4):22~28 |
| 半干旱区2种雪茄烟叶发酵后差异代谢物分析 |
| Differential metabolites analysis on two varieties of fermented cigar leaves in semi\|arid region |
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| DOI：10.7606/j.issn.1000-7601.2024.04.03 |
| 中文关键词: 雪茄品种 烟叶 非靶向代谢组学 差异代谢物 半干旱区 |
| 英文关键词:cigar varieties tobacco non\|targeted metabolomics differential metabolites semi\|arid region |
| 基金项目:中国烟草总公司陕西省烟草公司重大科技项目（SXYC-2022-KJ-01） |
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| 中文摘要: |
| 通过比较2个雪茄品种‘海研103’和‘青雪204’发酵后烟叶的代谢产物，探究不同品种烟叶间代谢组的差异。采用超高效液相色谱-四极杆飞行时间质谱联用技术(UHPLC-QTOF-MS)，对2个雪茄品种烟叶化学成分进行了非靶向代谢组学分析。结果表明：‘海研103’和‘青雪204’的代谢物相对含量存在明显差异。2个雪茄品种烟叶共筛选鉴定出179个差异代谢物，‘海研103’较‘青雪204’有114个代谢物显著上调，有65个显著下调。对前40个主要差异代谢物用PLS-DA法进行分析，鉴定为有机酸10种、氨基酸7种、酚酸类4种、核苷酸及类似物4种、碳水化合物3种、黄酮类3种、木脂素和香豆素3种，其他6种。结合KEGG通路分析，发现12条2个品种显著富集的代谢通路。 |
| 英文摘要: |
| By comparing the metabolites between two varieties of fermented cigar leaves ‘Haiyan 103’ and ‘Qingxue 204’, the metabolome differences between two varieties of cigar leaves were explored. In the study, non\|targeted metabolomic analysis of the chemical components of two fermented cigar leaves were analyzed by Ultra High\|Performance Liquid Chromatography\|Quadrupole\|Time of Flight Mass Spectrometric (UHPLC-QTOF-MS). The results indicated that, the metabolomes relative content of ‘Haiyan 103’ and ‘Qingxue 204’ were significantly different. A total of 179 differential metabolites were identified in two varieties of fermented cigar leaves, of which 114 metabolites were significantly up\|regulated in ‘Haiyan 103’ compared to ‘Qingxue 204’, while 65 were significantly down\|regulated. Forty major differential metabolites were qualitatively and quantitatively identified, including 10 organic acids, 7 amino acids, 4 phenolic acids, 4 nucleotides and analogues, 3 carbohydrates, 3 flavonoids, 3 lignans and coumarins, and 6 others. Based on KEGG pathway analysis, 12 significantly enriched pathways were identified. |
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