| 郭志婷,秦梦凡,李浩东,赵飞雪,李心茹,杜媛媛,包顺君,李鸿洋,徐佳丽,黄镇.甘蓝型油菜BnAR基因家族全基因组鉴定与响应低温胁迫的表达分析[J].干旱地区农业研究,2024,(6):27~37 |
| 甘蓝型油菜BnAR基因家族全基因组鉴定与响应低温胁迫的表达分析 |
| Genome\|wide identification of BnAR gene family and expression analysis under low temperature stress in Brassica napus L. |
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| DOI：10.7606/j.issn.1000-7601.2024.06.04 |
| 中文关键词: 甘蓝型油菜 BnAR基因家族 生物信息学 表达模式：低温胁迫 |
| 英文关键词:Brassica napus L. BnAR gene family bioinformatics expression pattern low temperature stress |
| 基金项目:国家重点研发计划项目（2022YFD1200400）；拉萨市重点科技计划项目（LSKJ202430） |
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| 中文摘要: |
| 为了解甘蓝型油菜（Brassica napus L.）中BnAR基因的基本信息、进化关系及其在低温胁迫下的表达模式，对甘蓝型油菜BnAR基因家族成员进行全基因组鉴定，并探究低温胁迫下家族成员的表达情况。结果表明：甘蓝型油菜参考基因组中共鉴定出14个BnAR基因家族成员，分布在8条染色体上，将其命名为BnAR-1~BnAR-14，可分为ADC和DapDC两个亚家族；启动子顺式作用元件分析发现，BnAR基因家族成员的启动子区含有多种与激素和胁迫响应相关的元件；甘蓝型油菜组内共线性分析发现11对基因具有共线性关系，Ka/Ks分析发现所有同源基因的比值均小于0.5，说明BnAR基因家族在进化过程中受到严格的纯化选择；转录组数据分析表明，14个BnAR基因均响应低温胁迫，其中BnAR-4、BnAR-5、BnAR-11和BnAR-13受低温胁迫程度最为强烈，荧光定量PCR验证结果与转录组数据高度一致，因此可将这4个基因作为候选基因进行后续的功能验证。 |
| 英文摘要: |
| To understand the basic information, evolutionary relationship and the function of BnAR genes under cold stress in Brassica napus L., the BnAR gene family of B.napus were identified in this study, and the expression of family members under low temperature conditions were explored. The results showed that 14 BnAR genes were identified in B.napus. They were distributed on eight chromosomes and renamed as BnAR-1 to BnAR-14, dividing into ADC and DapDC subgroups. Promoter cis\|element analysis showed that the promoter region of BnAR genes contained various elements related to hormone and stress response. Intra\|group collinearity analysis of B.napus showed that 11 pairs of genes had collinearity relationship, and Ka/Ks analysis showed that the ratio of all homologous gene pairs were less than 0.5, indicating that the BnAR gene family was subjected to strict purification selection in the evolutionary process. Transcriptome data analysis indicated that all 14 BnAR genes responded to low temperature stress, among which four genes, BnAR-4, BnAR-5, BnAR-11 and BnAR-13 were strongly subjected to low temperature stress. The results of qRT-PCR were highly consistent with the transcriptome data, so these four genes could be used as candidate genes for subsequent functional verification. |
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