刘志金,石凌旭,张佳,乔杰栋,于雅雯,袁昊,张锐,张建良,陈小飞.核桃腐烂病生防菌Streptomyes deccanensis的鉴定与应用[J].干旱地区农业研究,2024,(6):216~224
核桃腐烂病生防菌Streptomyes deccanensis的鉴定与应用
dentification and application of walnut rot biocontrol fungus Streptomyes deccanensis
  
DOI：10.7606/j.issn.1000-7601.2024.06.22
中文关键词:  核桃腐烂病  生防放线菌  促生作用
英文关键词:walnut rot  biocontrol bacterium  growth promoting effect
基金项目:国家级林业科技成果推广项目\[2019\]55；国家自然科学基金项目(31560007)；新疆生产建设兵团“三区”人才支持计划科技人员专项计划项目(BT1720230048G)
作者单位
刘志金 塔里木大学农学院，南疆有害生物综合治理兵团重点实验室，新疆 阿拉尔 843300 
石凌旭 塔里木大学农学院，南疆有害生物综合治理兵团重点实验室，新疆 阿拉尔 843300 
张佳 塔里木大学农学院，南疆有害生物综合治理兵团重点实验室，新疆 阿拉尔 843300 
乔杰栋 塔里木大学农学院，南疆有害生物综合治理兵团重点实验室，新疆 阿拉尔 843300 
于雅雯 塔里木大学农学院，南疆有害生物综合治理兵团重点实验室，新疆 阿拉尔 843300 
袁昊 塔里木大学农学院，南疆有害生物综合治理兵团重点实验室，新疆 阿拉尔 843300 
张锐 塔里木大学园艺与林学学院，新疆 阿拉尔 843300 
张建良 新疆生产建设兵团第一师阿拉尔市三团农业发展服务中心，新疆 阿拉尔 843011 
陈小飞 塔里木大学农学院，南疆有害生物综合治理兵团重点实验室，新疆 阿拉尔 843300 
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中文摘要:
      为了筛选出对核桃腐烂病具有高效防治效果、同时对核桃种子发芽具有促生作用的生防菌株，从新疆温宿县核桃林场核桃园采集土壤，采用稀释涂布法进行生防菌的分离与筛选。通过形态学特征、生理生化特征以及分子生物学方法对所分离的菌株进行物种鉴定，并研究其抗菌能力、防病效果以及对核桃种子发芽的促进作用。从土壤中分离到一株具有广谱抑菌能力的链霉菌F-04（CGMCC No.29519），经过分类鉴定，确定为德干链霉菌（Streptomyes deccanensis）。该菌对核桃腐烂病菌Cytospora chrysosperma的抑菌率为81.75%；其发酵滤液对核桃枝条腐烂病的防治效果可达87.24%；此外，该菌株的发酵滤液对核桃种子发芽有显著促进作用，在150 mL·L-1的浓度下，发芽率最高（85.32%），同时在该浓度处理下，坏种率最低（9.32%）。
英文摘要:
      To identify the biocontrol strains with high control effect on walnut rot and promote the germination of walnut seeds, soil was collected from the walnut orchard of a walnut forest farm in Wensu County, Xinjiang. Biocontrol bacteria were isolated and screened by a dilution coating method. The biocontrol bacteria were identified by morphological characteristics, physiological and biochemical characteristics combined with molecular biology, and their antibacterial ability, disease prevention effect and promoting effect on walnut seed germination were studied. A strain of Streptomyces F-04（CGMCC No. 29519）with broad\|spectrum antibacterial activity was isolated, and it was identified as Streptomyes deccanensis. The inhibitory rate of this strain against Cytospora chrysosperma was 81.75%, and its fermentation filtrate achieved an 87.24% control effect on walnut branch rot. At a concentration of 150 mg·mL-1, the fermentation filtrate resulted in the highest germination rate of 85.32%, while the seed failure rate was the lowest at 9.32%. This demonstrates a significant promoting effect on walnut seed germination.
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