耐盐碱土著菌的解钾性能及其对盐渍土钾素的影响研究(已上传)
Study on the potassium-solubilizing performance of saline-alkali tolerant indigenous bacteria and its effect on potassium in saline soil
投稿时间:2024-09-12  修订日期:2025-04-12
DOI:
中文关键词:  耐盐碱菌  盐渍土  解钾  钾长石
英文关键词:saline-alkali tolerant bacteria  saline soil  dissolve potassium  potassium feldspar
基金项目:国家自然科学基金项目(面上项目,重点项目,重大项目)
作者单位邮编
冉玥 西南科技大学 621010
代群威* 西南科技大学 621010
张永德 西南科技大学 
郭军 西南科技大学 
王维富 西南科技大学 
高俊 西南科技大学 
山静 西南科技大学 
蒋瑞阳 西南科技大学 
谢远杰 西南科技大学 
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中文摘要:
      土壤盐渍化不仅会造成土壤退化、土地资源短缺、生态环境恶化,还会限制农业可持续发展。微生物法是目前耗费资金和人力较少,且可持续的生态改良方法。盐渍土壤中存在大量含钾矿物,若对这些矿物中的钾素加以利用,不仅能改善环境问题,也能提高土壤肥力。本研究从硫酸盐型盐渍土中分离筛选出三株耐盐碱菌株,以土壤矿物钾长石为底物,在实验室开展批量实验,利用火焰原子吸收光度法、三维荧光光谱-平行因子分析法、扫描电镜(SEM)等,对其解钾性能和机理进行研究和分析,并挑选解钾性能最优菌株进行实验,探究菌株对盐渍土中钾素的变化影响。结果表明:三株菌均为革兰氏阳性芽孢杆菌,且均能耐受极端盐碱环境,解钾量能达到18.8 mg/L;三株菌均能使得发酵液pH降低,且对难溶性含钾矿物有一定程度的溶蚀作用,初步推测菌株通过分泌某种酸性物质溶解矿物;施菌后盐渍土壤有效钾含量提高13.76 mg/kg,该菌有可能作为盐渍土改良的候选菌株。本研究揭示了所选耐盐碱菌株对钾长石的溶解机理,探明了耐盐碱土著菌对盐渍土钾素的变化影响,为贫瘠盐渍土的改良提供了理论借鉴。
英文摘要:
      Soil salinization will not only cause soil degradation, land resource shortage, and ecological environment deterioration, but also limit the sustainable development of agriculture. Microbial method is a sustainable ecological improvement method with less cost and manpower. There are a large number of potassium-containing minerals in saline soil. If the potassium in these minerals is used, it can not only improve environmental problems, but also improve soil fertility. In this study, three salt-tolerant strains were isolated and screened from sulfate-type saline soil, and batch experiments were carried out in the laboratory with soil mineral potassium feldspar as the substrate. The potassium-solubilizing performance and mechanism were studied and analyzed by flame atomic absorption spectrometry, three-dimensional fluorescence spectroscopy-parallel factor analysis, SEM, etc., and the optimal potassium-solubilizing strain was selected for experiments to explore the effect of the strain on the change of potassium in saline soil. The results showed that the three strains were Gram-positive Bacillus, and could tolerate extreme saline-alkali environment, and the potassium release amount could reach 18.8 mg / L. The three strains can reduce the pH of the fermentation broth and have a certain degree of dissolution of insoluble potassium-containing minerals. It is preliminarily speculated that the strain dissolves minerals by secreting acidic substances. The available potassium in saline soil increased by 13.76 mg / kg after the application of the bacteria, and the bacteria could be used as a candidate strain for saline soil improvement. This study revealed the dissolution mechanism of the selected salt-tolerant strains on potassium feldspar, and explored the effect of salt-tolerant indigenous bacteria on the change of potassium in saline soil, which provided a theoretical reference for the improvement of barren saline soil.
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