| 吕小旭,杨璐,王大成,赵映书,关鉴茹.15个宁夏枸杞品种叶片抗旱指标的筛选和比较研究[J].干旱地区农业研究,2025,(1):42~53 |
| 15个宁夏枸杞品种叶片抗旱指标的筛选和比较研究 |
| Screening and comparison of leaf drought resistance indexes of 15 varieties of Lycium barbarum |
| |
| DOI：10.7606/j.issn.1000-7601.2025.01.05 |
| 中文关键词: 宁夏枸杞 品种 叶片显微结构 抗旱性综合评价 隶属函数 |
| 英文关键词:Lycium barbarum varieties leaf microstructure comprehensive evaluation of drought resistance membership function |
| 基金项目:全国第四次中药资源普查项目甘肃省崆峒区普查（GSZYPC201711）;甘肃静宁县普查（GSZYPC201804）；甘肃省高等教育教学成果培育项目（JXCGPY2022183） |
|
| 摘要点击次数: 0 |
| 全文下载次数: 0 |
| 中文摘要: |
| 以中宁县国家枸杞种质资源库中的15个宁夏枸杞品种为试验材料，测定5项叶片形态特征（叶面积LA、干质量DWT、鲜质量FW、比叶面积SLA、干鲜比LDMC），采用优化改进的石蜡切片技术测定10项叶显微结构特征（叶片厚度LT、上表皮细胞厚度TU、下表皮细胞厚度TL、栅栏组织厚度TPT、海绵组织厚度TST、栅栏组织厚度/海绵组织厚度P/S、叶片结构紧密度TLS、叶片结构疏松度LLS、上表皮占比PU、下表皮占比PL）,对这15项指标进行主成分分析，利用隶属函数对宁夏枸杞品种的抗旱性进行综合评价。结果表明：（1）15项指标抗旱系数的变异系数为12.900%～58.248%，其中变异系数最大的指标为LA，最小的为TPT。（2）相关性分析结果显示，LT与TLS、LLS、PU、PL呈极显著负相关关系；聚类分析结果表明，15项叶片指标分成3类，LLS、LA、SLA在各类别中相关指数最大，为宁夏枸杞抗旱性的关键指标；主成分分析可得，TLS、TST、LA、SLA、P/S、LDMC这6个主成分因子累积贡献率达到89.662%。综合可得，LLS、TLS、TST、LA、SLA、P/S、LDMC共7项叶形态及显微结构特征可作为宁夏枸杞抗旱性评价的关键指标。（3）各宁夏枸杞品种抗旱性强弱表现为：‘宁杞7号’＞‘宁农杞5号’＞‘宁杞9号’＞‘宁杞4号’＞‘宁农杞1号’＞‘宁杞3号’＞‘宁杞1号’＞‘宁杞6号’＞‘宁杞5号’＞‘宁杞10号’＞‘宁农杞4号’＞‘蒙杞1号’＞‘宁杞2号’＞‘宁杞8号’＞‘宁农杞9号’。 |
| 英文摘要: |
| Five leaf morphological characteristics—leaf area (LA), dry weight (DWT), fresh weight (FW), specific leaf area (SLA), and dry\|to\|fresh weight ratio (LDMC)—were calculated for 15 varieties of Lycium barbarum in Zhongning County. Ten leaf microstructure features\|leaf thickness (LT), thickness of upper epidermal cell (TU), thickness of lower epidermal cell (TL), thickness of palisade tissue (TPT), thickness of spongy tissue (TST), palisade tissue thickness/spongy tissue thickness (P/S), tightness of leaf structure (TLS), leaf structure porosity (LLS), proportion of upper epidermal (PU) and proportion of lower epidermal (PL) were determined by using the optimized and improved paraffin section technique. The drought resistance of Lycium barbarum cultivars was evaluated by using the membership function and principal component analysis of these 15 indexes. The results showed that: (1) The coefficient of variation for the drought resistance coefficients across 15 indices ranged from 12.900% to 58.248%. Among these, the index with the highest coefficient of variation was LA, while the index with the lowest was TPT. (2) According to the correlation analysis results, LT was significantly negatively correlated with TLS, LLS, PU and PL. The cluster analysis results showed that the 15 leaf indexes were divided into 3 categories, and LLS, LA and SLA had the highest correlation index among all categories, which were the key indexes of drought resistance of Lycium barbarum. Principal component analysis showed that the cumulative contribution rate of six principal component factors, including TLS, TST, LA, SLA, P/S and LDMC, reached 89.662%. LLS, TLS, TST, LA, SLA, P/S and LDMC were the key indicators for drought resistance evaluation of Lycium barbarum. (3)Drought resistance order of 15 Lycium barbarum varieties: Lycium barbarum ‘Ningqi-7’>‘Ningnongqi-5’>‘Ningqi-9’>‘Ningqi-4’>‘Ningnongqi-1’>‘Ningqi-3’>‘Ningqi-1’>‘Ningqi-6’>‘Ningqi-5’>‘Ningqi-10’>‘Ningnongqi-4’>‘Mengqi-1’>‘Ningqi-2’>‘Ningqi-8’>‘Ningnongqi-9’. The results of this study can help select excellent drought resistant varieties of Lycium barbarum ，reduce drought stress，and promote the development of Lycium barbarum industry. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| | |