| 邱应德,罗永忠,齐建伟,周晓彤,马超,于思敏.紫花苜蓿CNGC基因家族全基因组鉴定及干旱胁迫下的调控模式分析[J].干旱地区农业研究,2025,(1):54~68 |
| 紫花苜蓿CNGC基因家族全基因组鉴定及干旱胁迫下的调控模式分析 |
| Identification of the whole CNGC gene family in alfalfa and analysis of its regulatory patterns under drought stress |
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| DOI：10.7606/j.issn.1000-7601.2025.01.06 |
| 中文关键词: 同源四倍体紫花苜蓿 CNGC基因家族 蛋白质磷酸化 干旱胁迫 表达模式 |
| 英文关键词:tetraploid alfalfa CNGC gene family protein phosphorylation drought stress expression mode |
| 基金项目:国家自然科学基金（32160409） |
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| 中文摘要: |
| 环核苷酸门控通道蛋白（cyclic nucleotide\|gated channel proteins, CNGC）在Ca2+信号转导过程中发挥着重要作用。利用生物信息学和分子生物学的研究方法对四倍体紫花苜蓿（Medicago sativa‘Xinjiangdaye’）CNGC基因家族成员进行全基因组鉴定和干旱胁迫下的调控模式分析。结果表明：新疆大叶苜蓿基因组中共包含67个MsCNGC基因，不均匀地分布在30条染色体上，分属5个亚家族，共包含20个Motif，其启动子区域存在多种响应逆境胁迫的作用元件。新疆大叶苜蓿叶片中共表达了28个MsCNGC基因，有26个在干旱胁迫下不均匀上调，其中MsCNGC59表达量最高且在干旱胁迫下上调倍数最大。MsCNGCs含有29~120个磷酸化位点，能被LRR蛋白激酶磷酸化，MsLRRs与MsCNGCs基因表达之间呈显著的正相关关系。此外，Ca2+信号转导相关基因MsCaM、MsCML和MsCDPK表达量在干旱胁迫下显著上调。综上，四倍体紫花苜蓿MsCNGCs对干旱胁迫的响应包括转录调控和蛋白质的磷酸化调控，在干旱胁迫下能通过参与Ca2+信号转导途径提高紫花苜蓿的抗旱性。 |
| 英文摘要: |
| Cyclic nucleotide\|gated channel proteins (CNGC) play an important role in Ca2+ signal transduction. Genome\|wide identification of CNGC gene family members in tetraploid alfalfa (Medicago sativa ‘Xinjiangdaye’) and analysis of their regulatory patterns under drought stress were carried out using bioinformatics and molecular biology research methods. The results revealed that the ‘Xinjiangdaye’ alfalfa genome contains 67 MsCNGC genes, unevenly distributed across 30 chromosomes. These genes were classified into five subfamilies, contain 20 Motifs, and exhibit various regulatory elements in their promoter regions that respond to environmental stress. A total of 28 MsCNGC genes were expressed in the leaves of ‘Xinjiangdaye’ alfalfa, and 26 of them were unevenly up\|regulated under drought stress, among which MsCNGC59 was the highest expressed and the most multiplicatively up\|regulated under drought stress. The proteins of MsCNGCs contained 29~120 phosphorylation sites, which could be phosphorylated by the LRR protein kinase, and the gene expression of MsLRRs and MsCNGCs showed a significant positive correlation. In addition, the expression levels of Ca2+ signal transduction\|related genes MsCaM, MsCML and MsCDPK were found to be significantly up\|regulated under drought stress. In summary, the response of tetraploid alfalfa MsCNGCs to drought stress included transcriptional regulation and protein phosphorylation regulation, which can improve the drought resistance of alfalfa under drought stress by participating in the Ca2+ signal transduction pathway. |
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