| 邢艺凡,陈小飞,韩雷,史晓龙,刘春娟,刘畅,周宇飞.CaCl2引发对盐胁迫下高粱种子萌发期GA及ABA代谢的影响[J].干旱地区农业研究,2025,(1):76~84 |
| CaCl2引发对盐胁迫下高粱种子萌发期GA及ABA代谢的影响 |
| Effects of CaCl2 priming on GA and ABA metabolism during sorghum seed germination under salt stress |
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| DOI：10.7606/j.issn.1000-7601.2025.01.08 |
| 中文关键词: 高粱 盐胁迫 氯化钙引发 萌发期 赤霉素 脱落酸 |
| 英文关键词:sorghum salt stress CaCl2 priming germination period GA ABA |
| 基金项目:财政部和农业农村部国家现代农业产业技术体系建设专项（CARS-06-14.5-A17）；辽宁省藏粮于技重大专项（2023JH1/10200001-03-01） |
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| 中文摘要: |
| 以高粱品种‘辽杂15’为试验材料，通过CaCl2引发和相关激素抑制剂的应用，分析了盐胁迫下高粱种子的萌发特性、GA和ABA含量以及相关基因的表达变化。结果表明，盐胁迫显著抑制了高粱种子的萌发，种子平均发芽时间、50%发芽时间分别显著增加76.41%和92.75%；高粱萌发种子中GA3的含量显著降低63.01%，ABA的含量显著增加108.71%。CaCl2引发显著促进了盐胁迫下高粱种子的萌发，与盐胁迫相比，CaCl2引发上调了GA合成酶基因GA20ox1B、GA20ox1D和GA20ox2的表达水平以及ABA分解酶基因ABA8ox1、ABA8ox2和ABA8ox3的表达水平，使盐胁迫下高粱种子中GA3含量增加117.19%，ABA含量降低30.40%，使高粱种子的萌发率提高114.94%，芽长和根长分别显著增加265.73%和190.37%。在引发剂中加入GA合成或ABA分解抑制剂后，GA3含量显著降低28.29%，ABA含量显著增加31.49%，高粱种子萌发受到抑制;使用钙抑制剂处理后，GA3含量显著下降41.25%，ABA含量显著增加29.45%，进而抑制了高粱种子萌发。综上，CaCl2引发可通过调控GA合成和ABA分解促进盐胁迫下高粱种子的萌发。 |
| 英文摘要: |
| The sorghum variety ‘Liaoza 15’ was used as the test material. By employing CaCl2 priming and the application of relevant hormone inhibitors, we analyzed the germination characteristics of sorghum seeds under salt stress, the content of gibberellins (GA) and abscisic acid (ABA), and the expression changes of associated genes. The results showed that salt stress significantly inhibited the germination of sorghum seeds. The average germination time and 50% germination time of sorghum seeds were significantly increased by 76.41% and 92.75%, respectively. The content of GA3 in germinated sorghum seeds was significantly decreased by 63.01%, and the content of ABA was significantly increased by 108.71%. However, CaCl2 priming significantly promoted the germination of sorghum seeds under salt stress. Compared to the control under salt stress, CaCl2 priming upregulated the expression levels of GA synthesis genes GA20ox1B, GA20ox1D, and GA20ox2, as well as ABA catabolic genes ABA8ox1, ABA8ox2, and ABA8ox3. This led to a 117.19% increase in GA3 content and a 30.40% decrease in ABA content in sorghum seeds under salt stress, resulting in a 114.94% increase in germination rate, and a significant increase in shoot length and root length by 265.73% and 190.37%, respectively. When GA synthesis or ABA degradation inhibitors were added to the priming agent, GA3 content significantly decreased by 28.29%, and ABA content significantly increased by 31.49%, inhibiting the germination of sorghum seeds. After treatment with a calcium inhibitor, GA3 content significantly decreased by 41.25%, and ABA content significantly increased by 29.45%, further inhibiting the germination of sorghum seeds. In conclusion, CaCl2 priming promoted the germination of sorghum seeds under salt stress by regulating GA synthesis and ABA degradation. |
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