| 高梦哲,袁晓霞,杨冰洁,申奥龙,李华,冀照君.内蒙古东部地区乌拉尔甘草根瘤菌遗传多样性研究[J].干旱地区农业研究,2025,(1):125~131 |
| 内蒙古东部地区乌拉尔甘草根瘤菌遗传多样性研究 |
| Genetic diversity of rhizobia associated with Glycyrrhiza uralensis fisch in eastern Inner Mongolia |
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| DOI：10.7606/j.issn.1000-7601.2025.01.13 |
| 中文关键词: 根瘤菌 乌拉尔甘草 遗传多样性 内蒙古东部 |
| 英文关键词:rhizobia Glycyrrhiza uralensis genetic diversity eastern Inner Mongolia |
| 基金项目:国家自然科学基金（32170020，31860001）；内蒙古自治区科技计划项目（2021GG0005）；内蒙古自治区自然科学基金（2024JQ12，2022QN03030，2023MS03053）；内蒙古自治区人才开发基金项目（RC201901） |
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| 中文摘要: |
| 为探究乌拉尔甘草共生根瘤菌的微进化历程，温室条件下种植乌拉尔甘草捕捉当地土壤中根瘤菌，共分离得到根瘤菌21株，基于核心基因recA序列鉴定菌株的种属分别为豌豆根瘤菌（Rhizobium leguminosarum）、阿拉米根瘤菌（Rhizobium alamii）、阳陵根瘤菌（Rhizobium yanglingense）、菜豆根瘤菌（Rhizobium phaseoli）、圆明园慢生根瘤菌（Bradyrhizobium yuanmingense）、Rhizobium sp. I和Bradyrhizobium sp. II，其中Rhizobium sp. I为第一优势菌群，Bradyrhizobium sp. II为第二优势菌群，群落结构相对单一，核苷酸多态性（π）较低（范围为0.00205~0.01791，平均值为0.010406），遗传距离（Dxy）较远（范围为0.0688~0.1935，平均值为0.1483），基因交流指数（Nm）较低（范围为0.02~17.10，平均值为3.048）。综上所述，内蒙古东部地区乌拉尔甘草根瘤菌遗传多样性较高，在进化历史上基因交流频率较低，具有较强的遗传稳定性。 |
| 英文摘要: |
| To investigate the microevolution of symbiotic rhizobia associated with Glycyrrhiza uralensis, the plant was cultivated aseptically in greenhouse to isolate rhizobia from the local soil. A total of 21 rhizobial strains were isolated and identified as Rhizobium leguminosarum, Rhizobium alamii, Rhizobium yanglingense, Rhizobium phaseoli, Bradyrhizobium yuanmingense, Rhizobium sp. I and Bradyrhizobium sp. II. And Rhizobium sp. I was the predominant group, followed by Bradyrhizobium sp. II based on the core gene recA sequences. The community structure was relatively simple, with low nucleotide diversity (π) observed (range: 0.00205~0.01791, mean: 0.010406). Genetic differentiation (Dxy) was high, with values ranging from 0.0688 to 0.1935 (mean: 0.1483).The gene flow index (Nm) exhibited low values (range: 0.02~17.10, mean: 3.048). In conclusion, the high genetic diversity and low frequency of gene exchange suggest that the rhizobia associated with G. uralensis in eastern Inner Mongolia exhibit strong genetic stability throughout their evolutionary history. |
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