| 王玥,马永强.马铃薯黑腐果胶杆菌分离鉴定及室内药剂筛选[J].干旱地区农业研究,2025,(1):241~249 |
| 马铃薯黑腐果胶杆菌分离鉴定及室内药剂筛选 |
| Isolation and identification of potato black rot Pectobacterium atrosepticum and indoor fungicide screening |
| |
| DOI：10.7606/j.issn.1000-7601.2025.01.25 |
| 中文关键词: 马铃薯 黑胫病 病原鉴定 黑腐果胶杆菌 杀菌剂 |
| 英文关键词:potato black shank disease pathogen identification Pectobacterium atrosepticum bactericide |
| 基金项目:青海省应用基础研究项目（2023-ZJ-724） |
|
| 摘要点击次数: 0 |
| 全文下载次数: 1 |
| 中文摘要: |
| 通过病原菌培养特征、致病性验证和16S rRNA基因序列及特异性基因序列分析，对采自青海省海东市和西宁市马铃薯主产区16个乡镇的马铃薯黑胫病疑似病样进行分离和鉴定，并采用生长速率法测定7种杀菌剂对马铃薯黑胫病原菌的室内毒力。从采集的68株黑胫病疑似病样中共分离鉴定到15株黑腐果胶杆菌Pectobacterium atrosepticum。室内毒力测定结果表明7种化学药剂均对病原菌有不同程度的抑制作用，其中0.3%四霉素水剂、46%氢氧化铜可湿性粉剂和30%琥胶肥酸铜可湿性粉剂抑菌效果最好，EC50（对供试生物发生50%效果的药剂剂量或浓度）分别为1.355、1.403、1.835 μg·mL-1，其次为10%纳米银水剂、3%中生菌素可湿性粉剂、40%喹啉铜悬浮剂，EC50值分别为106.429、146.332、149.121 μg·mL-1，病原菌对10%苯醚甲环唑水分散粒剂敏感性最差，EC50值为1 384.943 μg·mL-1。 |
| 英文摘要: |
| Using pathogen culture characteristics, pathogenicity verification, 16S rRNA gene sequence comparison, and specific primer amplification detection, suspected cases of potato black shank disease were isolated and identified from 16 towns in Haidong and Xining of Qinghai Province. The indoor virulence of seven fungicides against the pathogen causing potato black shank was evaluated using the growth rate method. From 68 suspected black shank samples, a total of 15 strains of Pectobacterium atrosepticum were successfully isolated and identified.The results of laboratory virulence test showed that the pathogenic bacteria had different inhibitory effects on 7 chemical agents. Among them, 0.3% tetramycin water, 46% copper hydroxide wettable powder, and 30% copper succinate wettable powder had the best antibacterial effect, with EC50 values of 1.355 μg·mL-1, 1.403 μg·mL-1 and 1.835 μg·mL-1, respectively. It was followed by 10% aqueous nanosilver, 3% mesorhizobium wettable powder and 40% copper quinoline suspension, with EC50 values of 106.429 μg·mL-1, 146.332 μg·mL-1 and 149.121 μg·mL-1, respectively. The sensitivity of pathogens to 10% difenoconazole aqueous dispersion granules was the worst. The EC50 value was 1 384.943 μg·mL-1. The results of this study provide scientific basis for rational selection of pesticides in production and field control of potato black shank disease. |
| 查看全文 查看/发表评论 下载PDF阅读器 |