刘东,赵强,段莹,高展,孟瑾瑾,陈红刚,杜弢.基于多指标联合多元统计分析的不同花色唐古特大黄育种材料筛选[J].干旱地区农业研究,2025,(2):1~12
基于多指标联合多元统计分析的不同花色唐古特大黄育种材料筛选
Screening of Rheum tanguticum breeding materials with different flower colors based on multi\|index combined multivariate statistical analysis
  
DOI:10.7606/j.issn.1000-7601.2025.02.01
中文关键词:  唐古特大黄  育种材料筛选  花色  根部性状  多指标联合多元统计分析法
英文关键词:Rheum tanguticum Maxim.  screening of breeding materials  color scheme  root traits  joint multivariate statistical analysis
基金项目:国家中药材产业技术体系建设专项资金(CARS-21);甘肃省科技特派团专项“唐古特大黄种子丸粒化技术研究及栽培模式创新示范”(23CXNA0033)
作者单位
刘东 甘肃中医药大学甘肃 兰州 730000 
赵强 甘肃中医药大学甘肃 兰州 730000 
段莹 甘肃中医药大学甘肃 兰州 730000 
高展 甘肃中医药大学甘肃 兰州 730000 
孟瑾瑾 齐鲁理工学院山东 济南 250000 
陈红刚 甘肃中医药大学甘肃 兰州 730000 
杜弢 甘肃中医药大学甘肃 兰州 730000 
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中文摘要:
      以18个不同花色唐古特大黄为研究材料,通过观察和测定根部性状及9种化合物含量,基于相关性分析、主成分分析、熵权TOPSIS法分析,对唐古特大黄育种材料进行综合质量评价,筛选表现优良的唐古特大黄育种材料。结果表明,QH003yyf(S2)、TGT(S14)、ZH002QS(S18)等材料无病害、单根质量高(0.97~1.54 kg)、须根和芦头粗(31.72~36.00 mm,58.32~68.47 mm)且长(30.5~36.1 cm,5.1~6.8 cm);18个不同花色唐古特大黄根部的大黄素、大黄酸、大黄酚、芦荟大黄素、大黄素甲醚的总含量符合药典标准要求,其中QH002yyf(S16)、DH002yyfA(S3)、HS002yyf(S1)、TGTYS(S6)、TGT(S14)、ZH001DH(S8)共6个材料的9种化合物总含量高,分别为48.86、46.41、45.88、44.96、44.23、43.81 mg·g-1;熵权TOPSIS法分析综合排序前2位的品系为QH004yyf(S7)和ZH002QS(S18)。综上,TGT(S14)和ZH002QS(S18)可作为唐古特大黄品种选育和栽培的基础材料。本研究建立的基于多指标联合多元统计分析筛选不同花色唐古特大黄育种材料的评价模式,也可用于其他育种材料的筛选评价。
英文摘要:
      To identify suitable breeding materials of Rheum tanguticum, eighteen Rheum tanguticum Maxim. plants with different flower colors were selected as research materials. Comprehensive quality evaluation was conducted by assessing root traits and quantifying the contents of nine compounds, using correlation analysis, principal component analysis, and the entropy weighted TOPSIS method. The results showed that QH003yyf (S2), TGT (S14), and ZH002QS (S18) were disease\|free, and had large single root mass (0.97~1.54 kg), thick and long fibrous roots and reeds (31.72~36.00 mm, 30.5~36.1 cm; 58.32~68.47 mm, 5.1~6.8 cm). The total contents of emodin, rhein, chrysophanol, aloeemodin, and physcion contained in roots of 18 R. tanguticum Maxim. were in accordance with Pharmacopoeia standards. QH002yyf (S16), DH002yyfA (S3), HS002yyf (S1), TGTYS (S6), TGT (S14), and ZH001DH (S8) were high in the total content of the nine compounds, in the order of 48.86, 46.41, 45.88, 44.96, 44.23, 43.81 mg·g-1. The top 2 of the comprehensive ranking analyzed by entropy weight TOPSIS method were QH004yyf (S7) and ZH002QS (S18). In this study, TGT (S14) and ZH002QS (S18) were selected as the foundational materials for breeding and cultivating Rheum tanguticum varieties. The evaluation model, established through multi\|indicator joint multivariate statistical analysis for screening different flower colors in Rheum tanguticum breeding materials, can also be applied to the screening and evaluation of other breeding materials.
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