| 谢文龙,王彦芹.白麻NAC基因家族全基因组鉴定及其干旱胁迫下表达分析[J].干旱地区农业研究,2025,(2):54~65 |
| 白麻NAC基因家族全基因组鉴定及其干旱胁迫下表达分析 |
| Genome\|wide identification of the NAC gene family in Apocynum pictum and its expression analysis under drought stress |
| |
| DOI：10.7606/j.issn.1000-7601.2025.02.06 |
| 中文关键词: 白麻 NAC基因家族 干旱胁迫 基因差异表达 |
| 英文关键词:Apocynum pictum NAC gene family drought stress gene different expression |
| 基金项目:新疆维吾尔自治区南疆重点产业创新发展支撑计划项目（2022DB012）；新疆生产建设兵团第一师农业农村局农业领域项目（2023ZD04040-4-2） |
|
| 摘要点击次数: 200 |
| 全文下载次数: 117 |
| 中文摘要: |
| 基于白麻基因组数据，在白麻全基因组范围内鉴定了53个NAC基因家族成员，并对其染色体分布、系统发育、基因和蛋白结构、蛋白理化性质与功能、顺式作用元件进行预测分析，并结合干旱胁迫下的转录组数据分析NAC基因的表达。结果显示，白麻的ApNAC基因家族分布在10条染色体中，包括7对片段复制形成的共线性基因对和2对串联重复基因对。53个ApNAC家族成员可以分为15个亚家族，每个成员都含有5个以上的保守基序，同一个亚家族成员有着相似的基因结构。白麻NAC蛋白属于亲水性蛋白，亚细胞定位结果显示其以转录因子的形式发挥功能，并富集在DNA特异性结合功能以及木质部、叶片和细胞壁的发育调节功能中。启动子区域的顺式作用元件分析表明，ApNAC基因家族与脱落酸、干旱胁迫以及光响应调控相关。在30% PEG-6000诱导的干旱胁迫下，26个ApNAC成员在不同阶段发生差异表达，其中21个基因整体上调，5个基因下调。 |
| 英文摘要: |
| Based on the genome data of Apocynum pictum, this study identified 53 members of the NAC gene family in A. pictum. Comprehensive predictive analyses were conducted to examine their chromosomal distribution, phylogenetic relationships, gene and protein structures, physicochemical properties, functions, and cis\|acting elements. Additionally, transcriptome data were analyzed to assess NAC gene expression under drought stress. The results revealed that the ApNAC gene family of Apocynum pictum was distributed on 10 chromosomes, including 7 pairs of collinearity genes formed from segmental duplications and 2 pairs of tandem duplicated genes. The 53 members of the ApNAC family were classified into 15 subfamilies, each member containing more than 5 conserved motifs, with similar gene structures within the same subfamily. The NAC proteins of Apocynum pictum were hydrophilic protein, with subcellular localization results indicating their functional role as transcription factors, enriched in DNA-specific binding functions as well as regulating development in xylem, leaves, and cell walls. Analysis of cis\|acting elements in the promoter region revealed the association of the ApNAC gene family with regulation of abscisic acid, drought stress, and light responses. Under drought stress induced by 30% PEG-6000, 26 members of ApNAC exhibited differential expression across various stages, with 21 members upregulated and 5 downregulated. |
| 查看全文 查看/发表评论 下载PDF阅读器 |