| 张斌.大豆转录因子GmMYB46调控植物耐旱性的功能研究[J].干旱地区农业研究,2025,(3):45~51 |
| 大豆转录因子GmMYB46调控植物耐旱性的功能研究 |
| Functional study of the soybean transcription factor GmMYB46 in regulating plant drought tolerance |
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| DOI：10.7606/j.issn.1000-7601.2025.03.05 |
| 中文关键词: 大豆 转录因子 GmMYB46基因 耐旱性 |
| 英文关键词:soybean transcription factor GmMYB46 gene drought tolerance |
| 基金项目:湖南省自然科学基金项目（2024JJ7190）；永州市指导性科技计划项目（2020-YZKJ-001） |
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| 中文摘要: |
| 为解析大豆转录因子MYB家族成员GmMYB46基因在干旱胁迫应答中的调节机制，通过荧光定量PCR检测其在PEG模拟干旱处理的大豆中的表达水平。克隆GmMYB46基因编码序列构建过表达载体，利用农杆菌介导转化野生型拟南芥，在正常培养和干旱处理条件下观察转基因和野生型（WT）拟南芥植株的生长情况，测定叶片电导率、可溶性糖和脯氨酸含量以及抗氧化酶活性，观察对比叶片气孔开度。结果表明：GmMYB46受干旱胁迫诱导在根和叶中的表达量显著上调，最高达8.9倍和16.3倍。成功获得GmMYB46基因过表达的拟南芥植株OE，在培养基和营养土培养模式下OE植株耐旱性均明显增强，其长势优于WT植株。干旱处理后，OE植株电导率较WT降低46.1%；可溶性糖和脯氨酸含量均显著高于WT植株，分别增加54.9%和225.0%；超氧化物歧化酶、过氧化物酶和过氧化氢酶活性也明显上升，在根中分别增加8.8%、14.6%和47.0%，叶中则分别增加11.2%、69.2%和21.2%。说明大豆GmMYB46基因可能与细胞渗透调节、抗氧化酶活性以及气孔开度等生理过程的调节相关。 |
| 英文摘要: |
| To elucidate the functional mechanism of the GmMYB46 gene, a member of the soybean MYB transcription factor family, in response to drought stress, quantitative real\|time PCR was used to analyze its expression levels in soybeans subjected to PEG\|simulated drought conditions. The coding sequence of the GmMYB46 gene was cloned to construct an overexpression vector, and the wild Arabidopsis was transformed via Agrobacterium. Growth patterns of transgenic and wild Arabidopsis plants under normal cultivation and drought stress were observed. Leaf electrolyte leakage, soluble sugar, proline content and antioxidant enzyme activity were measured, and stomatal aperture was observed and compared. The results showed that drought stress significantly upregulated GmMYB46 in roots and leaves, increasing its expression by up to 8.9-fold and 16.3-fold, respectively. Arabidopsis plants with overexpression of the GmMYB46 gene (OE) were successfully obtained, and the drought tolerance of these plants was significantly enhanced under both medium and nutrient soil cultural modes, and its growth was better than that of WT plants. Post drought stress, the electrolyte leakage of OE plants was 46.1% lower than that of WT, the contents of soluble sugar and proline were significantly higher than those of WT, increased by 54.9% and 225.0%, respectively. The activities of superoxide dismutase, peroxidase, and catalase were also significantly increased, increasing by 8.8%, 14.6% and 47.0% in root, and by 11.2%, 69.2% and 21.2% in leaf, respectively. These results indicate that the soybean transcription factor GmMYB46 enhances plant drought tolerance by increasing soluble sugar and proline content, activating antioxidant enzymes, maintaining membrane integrity, regulating osmotic pressure, scavenging reactive oxygen species, and reducing water loss. |
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