| 管丽丽,况勇,肖逸,孙照龙,甘德芳.过表达黄瓜CsAGO1c基因降低拟南芥植株的耐旱性[J].干旱地区农业研究,2025,(3):52~59 |
| 过表达黄瓜CsAGO1c基因降低拟南芥植株的耐旱性 |
| Overexpression of the cucumber CsAGO1c gene reduces drought tolerance in Arabidopsis thaliana |
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| DOI：10.7606/j.issn.1000-7601.2025.03.06 |
| 中文关键词: 黄瓜 CsAGO1c基因 过表达 拟南芥 耐旱性 |
| 英文关键词:cucumber CsAGO1c gene overexpression Arabidopsis drought tolerance |
| 基金项目:安徽省自然科学研究重点项目（2023AH051056） |
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| 中文摘要: |
| 克隆黄瓜CsAGO1c基因并研究干旱胁迫下的表达情况，构建CsAGO1c过表达载体农杆菌介导法转化拟南芥，研究干旱胁迫下CsAGO1c过表达拟南芥的表型及生理变化。结果表明：干旱胁迫下，CsAGO1c基因在黄瓜根中的表达水平先下降后上升，CsAGO1c表达水平在6 h下调57%、在12 h下调64%、在24 h上调4.24倍；在叶中呈现先下降后上升再下降的趋势，CsAGO1c表达水平在18 h上调1.16倍。过表达拟南芥种子较野生型提早萌发，渗透试验显示，400 mmol·L-1甘露醇处理明显抑制OE2根长的生长，其长度为WT的54%；离体叶片失水率在3、4、5 h时分别为WT的1.21倍、1.27倍、1.25倍，均显著高于野生型。另外，干旱胁迫下过表达拟南芥叶片中超氧化物歧化酶活性约为WT的64%，过氧化物酶活性约为WT的51%，低于野生型。OE2的丙二醛含量约为WT的2.65倍，显著高于野生型。说明异源表达CsAGO1c降低了拟南芥的耐旱性。 |
| 英文摘要: |
| CsAGO1c was cloned from the inbred line of cucumis variety ‘Xintaimici’, and its expression was analyzed under drought stress. An overexpression vector for CsAGO1c was constructed and used to transform Arabidopsis via Agrobacterium\|mediated transformation. The phenotypic and physiological changes of the CsAGO1c\|overexpressing plants were then studied under drought stress conditions. The results showed that the expression level of CsAGO1c in cucumber root first decreased and then increased. The expression level of CsAGO1c was down\|regulated by 57% at 6 h，down\|regulated by 64% at 12 h，and up\|regulated by 4.24 times at 24 h. The expression level of CsAGO1c in the leaves decreased first, then increased and then decreased, and the expression level of CsAGO1c increased 1.16 times at 18 h. Overexpressed Arabidopsis seeds germinated earlier than the wild type. The penetration test showed that 400 mmol·L-1 mannitol treatment significantly inhibited the growth of OE2 root length，which was 54% that of WT. The water loss rate of isolated leaves was 1.21, 1.27, and 1.25 times higher than that of the wild type (WT) at 3, 4, and 5 hours, respectively, showing a significantly greater loss compared to WT at all three time points. Additionally, under drought stress, the activities of superoxide dismutase and peroxidase in the overexpressing Arabidopsis lines were approximately 64% and 51% of those in WT, respectively, both significantly lower than WT levels. Meanwhile, the malondialdehyde content in OE2 was about three times higher than that of WT, indicating a marked increase. These findings suggest that heterologous expression of CsAGO1c reduces drought tolerance in Arabidopsis. This study provides valuable theoretical insight into the function of the CsAGO1c gene in cucumber and offers a foundation for breeding new drought\|resistant cucumber varieties. |
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