根际促生菌株Pantoea cypripedii TA1对小麦耐旱性的影响

李海洋; 杨姗姗; 桑萌; 杨光宇; 郭俏; 来航线

李海洋,杨姗姗,桑萌,杨光宇,郭俏,来航线.根际促生菌株Pantoea cypripedii TA1对小麦耐旱性的影响[J].干旱地区农业研究,2025,(4):32~42

根际促生菌株Pantoea cypripedii TA1对小麦耐旱性的影响

Effect of growth\|promoting rhizobacteria Pantoea cypripedii TA1 on drought tolerance of wheat

DOI：10.7606/j.issn.1000-7601.2025.04.04

中文关键词: 小麦根际促生细菌耐旱性渗透调节基因表达

英文关键词:wheat growth\|promoting rhizobacteria drought tolerance osmotic regulation gene expression

基金项目:陕西省农业农村厅科技项目（K3380220129）；商洛学院博士科研启动项目(24SKY016)

作者	单位
李海洋	西北农林科技大学资源环境学院，陕西杨凌 712100；商洛学院城乡规划与建筑工程学院，陕西商洛 726000；商洛市生态环境技术研究中心，陕西商洛 726000
杨姗姗	西北农林科技大学资源环境学院，陕西杨凌 712100
桑萌	西北农林科技大学资源环境学院，陕西杨凌 712100
杨光宇	西北农林科技大学资源环境学院，陕西杨凌 712100
郭俏	西北农林科技大学资源环境学院，陕西杨凌 712100
来航线	西北农林科技大学资源环境学院，陕西杨凌 712100

摘要点击次数: 273

全文下载次数: 176

中文摘要:

采用钵体栽培试验，以抗旱性有差异的小麦品种‘西农979’和‘小偃22号’为材料，研究不同干旱胁迫程度下，根际促生细菌杓兰泛菌Pantoea cypripedii TA1对小麦生长、干旱相关基因及叶片渗透调节物质的影响。结果表明，中度干旱胁迫下，与不接菌处理相比较，P. cypripedii TA1使‘小偃22’和‘西农979’小麦植株的地上部鲜质量分别增加19.30%和16.67%，叶绿素含量分别增加24.81%和12.50%，叶片相对含水量分别增加9.84%和7.36%，叶片丙二醛含量分别降低20.69%和13.33%。干旱胁迫5 d时，TA1处理显著上调‘小偃22’小麦干旱响应基因TaDREB2A、TaLEA、TaP5CS、AKT1、TaMYB33和SnRK2的表达，增幅分别为79.07%、24.04%、20.23%、41.68%、32.03%和16.77%，而‘西农979’上述基因上调幅度小于‘小偃22’；此外，干旱胁迫条件下，TA1还使‘小偃22’的K⁺、Ca²⁺、Mg²⁺、脯氨酸和可溶性蛋白含量分别显著增加8.95%、10.28%、6.61%、20.77%和23.76%，且增幅高于干旱敏感型小麦‘西农979’（4.66%、9.82%、3.13%、13.03%和22.09%）。综上，根际促生细菌TA1通过增强小麦的渗透调节能力，可更有效地提高耐旱小麦‘小偃22’的干旱耐受性。

英文摘要:

Two wheat varieties, ‘Xinong 979’ and ‘Xiaoyan 22’, differing in their drought tolerance, were used as materials in the bowl cultivation experiment. This study investigated the effects of Pantoea cypripedii TA1 on wheat growth traits, drought\|related gene expression, and osmotic adjustment substances in leaf tissues under varying levels of drought stress. The results showed that under moderate drought conditions, compared with the non\|inoculation treatments, inoculation with P. cypripedii TA1 increased the aboveground fresh mass of ‘Xiaoyan 22’ and ‘Xinong 979’ by 19.30% and 16.67%, chlorophyll content by 24.81% and 12.50%, and leaf relative water content by 9.84% and 7.36%. Notably, malondialdehyde content decreased by 20.69% and 13.33%, respectively. Under five days of drought stress, TA1 treatment significantly upregulated the expression of drought responsive genes (TaDREB2A, TaLEA, TaP5CS, AKT1, TaMYB33, and SnRK2) in ‘Xiaoyan 22’ wheat by 79.07%, 24.04%, 20.23%, 41.68%, 32.03%, and 16.77%, respectively. However, ‘Xinong 979’ exhibited lower upregulation of these genes compared to ‘Xiaoyan 22’. Additionally, TA1 treatment enhanced concentrations of K⁺, Ca²⁺, Mg²⁺, proline, and water\|soluble protein by 8.95%, 10.28%, 6.61%, 20.77%, and 23.76%, respectively, in ‘Xiaoyan 22’, with greater increases compared to drought\|sensitive ‘Xinong 979’ (4.66%, 9.82%, 3.13%, 13.03%, and 22.09%). In conclusion, the rhizobacterium TA1 enhances wheat’s osmotic adjustment capacity, particularly benefiting ‘Xiaoyan 22’ in improving drought tolerance.

查看全文查看/发表评论下载PDF阅读器