| 贾子健,王宝强,桂金凤,朱晓林,陈立飞,魏小红,赵颖.NO介导下藜麦LAZ1基因家族生信分析及盐碱胁迫下的表达模式[J].干旱地区农业研究,2025,(4):43~52 |
| NO介导下藜麦LAZ1基因家族生信分析及盐碱胁迫下的表达模式 |
| Bioinformatics analysis and expression patterns of the LAZ1 gene family in quinoa under saline\|alkali stress mediated by nitric oxide |
| |
| DOI：10.7606/j.issn.1000-7601.2025.04.05 |
| 中文关键词: 藜麦 LAZ1基因家族 NO 生信分析 盐碱胁迫 |
| 英文关键词:quinoa LAZ1 gene family NO bioinformatics analysis saline\|alkali stress |
| 基金项目:国家自然科学基金（32301775）；甘肃农业大学省部共建干旱生境作物学国家重点实验室开放基金课题(GSCS-2021-10)；甘肃省自然科学基金（23JRRA1426） |
|
| 摘要点击次数: 863 |
| 全文下载次数: 1081 |
| 中文摘要: |
| 利用生物信息学方法，从藜麦基因组中鉴定LAZ1基因家族成员，并对其基本理化特性、二级和三级结构、系统进化关系、染色体定位、基因复制、基因结构和启动子区顺式作用元件等进行分析。利用转录组数据结合RT-qPCR分析LAZ1家族基因在混合盐碱胁迫和外源NO处理下不同器官和时间的表达模式。结果显示，在藜麦基因组中共鉴定到11个LAZ1基因，系统发育分析将LAZ1基因家族分为3个亚家族；片段复制是该基因家族进化的主要原因，并在进化过程中经历了强烈的纯化选择；在转录组分析基础上，对其中6个LAZ1基因在盐碱胁迫和NO处理下的RT-qPCR鉴定分析表明，不同的LAZ1基因在不同组织中表达，响应混合盐碱并受到NO的正向调控，其中CqLAZ1-9在盐碱胁迫下根中的相对表达量提高了129.6倍。综上所述，本研究从藜麦基因组中鉴定了11个LAZ1家族成员，不同成员在不同组织中具有不同的表达模式，其中CqLAZ1-9响应盐碱胁迫和外源NO的能力最强。 |
| 英文摘要: |
| The LAZ1 gene family members were identified from the quinoa genome using bioinformatics methods, and their basic physicochemical properties, secondary and tertiary structures, phylogenetic relationships, chromosome localization, gene duplication, gene structure, and promoter cis\|acting elements were analyzed. Transcriptome data coupled with RT-qPCR were used to analyze the expression patterns of LAZ1 family genes in different organs and times under mixed saline\|alkali stress and exogenous NO treatment. The results showed that a total of 11 LAZ1 genes were identified in the quinoa genome, and the LAZ1 gene family was divided into 3 subfamilies by phylogenetic analysis. Fragment replication is the main reason for the evolution of this gene family, and it had undergone strong purification selection in the evolutionary process. On the basis of transcriptomic analysis, RT-qPCR analysis of 6 LAZ1 genes under salt\|alkali stress and NO treatment showed that the expression of different LAZ1 genes in different tissues was responsive to mixed salt\|alkali and positively regulated by NO, and the relative expression of CqLAZ1-9 in roots under salt\|alkali stress was increased by 129.6 times. In summary, this study identified 11 members of the LAZ1 family from the quinoa genome, and different members showed different expression patterns in different tissues, among which CqLAZ1-9 has the strongest ability to saline\|alkali stress and exogenous NO treatment. |
| 查看全文 查看/发表评论 下载PDF阅读器 |