| 李怀珠,韩其晟,王平,赵琴,司玉芳,刘建超,姚娅婷,张心雨.北柴胡根腐病发展过程中根系微生物群落组成变化规律研究[J].干旱地区农业研究,2025,(4):251~260 |
| 北柴胡根腐病发展过程中根系微生物群落组成变化规律研究 |
| Composition and succession dynamics of root microbial community during root rot development in Bupleurum chinense |
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| DOI：10.7606/j.issn.1000-7601.2025.04.26 |
| 中文关键词: 北柴胡 根腐病 根系微生物组 真菌多样性 细菌多样性 |
| 英文关键词:Bupleurum chinense root rot root microbiome fungal diversity bacterial diversity |
| 基金项目:陕西省科技厅重点研发计划一般项目（2023-YBNY-104）；陕西省科技厅技术创新引导专项（2024ZC-YYDP-87）；陕西省教育厅基础科学（化学、生物学）研究院基础科学研究计划项目（22JHQ050）；陕西省教育厅高校青年创新团队项目（23JP188，24JP203）；陕西省教育厅高校工程研究中心项目（24JR162）；陕西省教育厅高校青年创新团队（2022）；陕西省教育厅黄土高原道地药材陕西省高校工程研究中心（2023） |
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| 中文摘要: |
| 为探究北柴胡（Bupleurum chinense DC.）感染根腐病后微生物群落变化规律，采用高通量测序技术，系统分析了健康、发病初期和发病末期北柴胡根周土、根际土及根内真菌与细菌群落结构、群落动态变化及主要差异群落的功能。结果表明：根腐病主要影响根内微生物群落结构，随着病害发展，根内真菌多样性指数逐渐降低，Chao1与ACE指数分别下降65.69%与65.75%。其中，镰刀菌属（Fusarium）的相对丰度由健康组的0.47%升至发病末期的9.63%，腐皮镰刀菌（F. solani）为发病末期差异类群中LDA值最高的菌种，推测其可能为北柴胡根腐病的主要致病菌。相比之下，根内细菌多样性随病害发展呈上升趋势，Chao1与ACE指数分别增长90.18%与86.52%。其中根瘤菌属复合群（Allorhizobium\|Neorhizobium\|Pararhizobium\|Rhizobium）为发病末期特有差异类群，相对丰度增长达106.22%，推测北柴胡可能会通过招募有益细菌增强其抗病性。 |
| 英文摘要: |
| To explore the variation of microbial community after root rot infection of Bupleurum chinense DC, high\|throughput sequencing was employed to systematically analyze the fungal and bacterial community structures, temporal variations, and functional profiles of key differential taxa in bulk soil, rhizosphere soil, and root of B. chinense at healthy, early\|stage, and late\|stage of disease. The results showed that root rot predominantly affected the microbial communities inside the root. As the disease progressed, the diversity of root\|associated fungal communities significantly declined, with Chao1 and ACE indices decreasing by 65.69% and 65.75%, respectively. The relative abundance of the genus Fusarium increased markedly from 0.47% in healthy samples to 9.63% in the late\|stage infected samples. F.solani had the highest LDA score among the late\|stage differential groups, suggesting that it may be the primary pathogenic bacterium of root rot in B. chinense. In contrast, bacterial diversity in roots increased with disease progression, with Chao1 and ACE indices rising by 90.18% and 86.52%, respectively. Notably, the Allorhizobium\|Neorhizobium\|Pararhizobium\|Rhizobium complex was identified as a specific and significantly enriched taxon in the late\|stage differential group, showing a 106.22% increase in relative abundance. It is speculated that B. chinense may enhance its disease resistance by recruiting beneficial bacteria. |
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