| 李蔚佳,于淼,魏嘉谊,马世乐,周阳,吴许,关维明,杜吉到,杜艳丽.外源6-BA对盐碱胁迫下大豆种子和幼苗生理特性和相关基因表达的影响[J].干旱地区农业研究,2025,(5):47~58 |
| 外源6-BA对盐碱胁迫下大豆种子和幼苗生理特性和相关基因表达的影响 |
| Effects of exogenous 6-BA on physiological characteristics and related gene expression of soybean seeds and seedlings under saline\|alkali stress |
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| DOI：10.7606/j.issn.1000-7601.2025.05.05 |
| 中文关键词: 大豆 6-BA 盐碱胁迫 种子萌发 幼苗 抗逆生理 |
| 英文关键词:soybean 6-BA saline\|alkali stress seed germination seedling anti\|stress physiology |
| 基金项目:省基金:东北北部高油大豆促油增产关键技术研究（2023ZD0403106）；黑龙江省自然科学基金联合引导项目（LH2024C077）；黑龙江八一农垦大学引进人才科研启动计划基金(XYB202006)；省基金:耐盐碱大豆种质的应用及抗盐碱栽培技术示范(LJGXCG2022-111)；黑龙江八一农垦大学研究生创新科研项目（NXYYJSCX2023-Y06） |
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| 中文摘要: |
| 以大豆品种‘合农95’和‘牛眼睛’为试验材料，通过种子萌发和幼苗生长试验，比较盐碱胁迫下外源6-苄基腺嘌呤（6-BA）对种子萌发、生长,抗氧化系统、内源激素调节其及相关基因表达等的影响，探讨外源6-BA缓解盐碱胁迫下大豆种子萌发障碍和幼苗生长抑制的作用机理。结果表明：盐碱胁迫处理能够诱导大豆种子和幼苗中ROS积累，导致细胞氧化损伤现象，造成萌发期大豆的胚根长、干物质量、胚根体积、胚根面积和胚根直径较正常处理分别下降78.34%~81.00%、52.31%~63.40%、67.37%~80.62%、85.15%~85.20%和15.49%~45.52%。60 μmol·L-1的6-BA能通过调控胚根抗氧化防御系统和ROS积累，提高两个大豆品种在盐碱胁迫下的发芽率、发芽势、发芽指数和活力指数，增加幅度分别为33.33%、7.32% ~ 51.85%、20.76% ~ 32.27%和2.80% ~ 49.09%。外源添加6-BA能够改变不同大豆品种中内源激素（包括tZ、GA3、IAA 和ABA）的含量，在‘合农95’中的激素含量增加7.85%~16.37%，‘牛眼睛’中降低5.56%~12.77%，同时通过调控细胞分裂素代谢和信号调节相关基因的表达水平缓解盐碱胁迫对胚根生长的抑制，提升胚根干物质量、根体积、根面积和根直径，增幅分别为88.71%~145.55%、117.90%~465.00%、78.64%~98.87%和124.82%~235.48%。外源6-BA处理较盐碱处理叶片的叶色值和气孔导度分别提升77.90%~89.62%和118.85%~125.67%，有效增强大豆幼苗的光合作用，提高大豆幼苗抗氧化能力，进而促进地下根瘤形成和根系生长，使根长和干物质量分别增加1.55%~17.00%和70.43%~75.36%。 |
| 英文摘要: |
| This study investigated the mechanism of exogenous 6-benzyladenine (6-BA) in alleviating salt\|alkali stress on soybean seed germination and seedling growth using two soybean cultivars, ‘Henong 95’ and ‘Niuyanjing’. Through seed germination and seedling growth experiments, the effects of salt\|alkali stress and 6-BA treatment on germination parameters, growth indicators, antioxidant systems, endogenous hormone regulation, and related gene expression were compared. The results showed that salt\|alkali stress induced ROS accumulation in soybean seeds and seedlings, causing oxidative damage and significant reductions in radicle length (78.34%~81.00%), dry mass (52.31%~63.40%), radicle volume (67.37%~80.62%), radicle surface area (85.15%~85.20%), and root diameter (15.49%~45.52%) compared to controls. Application of 60 μmol·L-1 6-BA enhanced salt\|stress tolerance by regulating the antioxidant defense system and ROS accumulation, and improved germination rate (33.33%), germination potential (7.32%~51.85%), germination index (20.76%~32.27%), and vigor index (2.80%~49.09%) in both cultivars. Exogenous 6-BA modified endogenous hormone levels (tZ, GA3, IAA, and ABA), increasing their contents by 7.85%~16.37% in ‘Henong 95’ while decreasing them by 5.56%~12.77% in ‘Niuyanjing’. This treatment alleviated radicle growth inhibition by regulating cytokinin metabolism and signaling\|related genes, significantly improving radicle dry matter mass (88.71%~145.55%), root volume (117.90%~465.00%), root surface area (78.64%~98.87%), and root diameter (124.82%~235.48%). Compared to salt\|alkali treatment alone, 6-BA application increased leaf color value (77.90%~89.62%) and stomatal conductance (118.85%~125.67%), effectively enhancing photosynthetic capacity and antioxidant activity in seedlings. These improvements promoted underground nodule formation and root growth, resulting in increased root length (1.55%~17.00%) and dry matter mass (70.43%~75.36%). |
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