| 胡玉蓉,邱黛玉,马澜,陈鲜鲜,徐锋宾.外源物质预处理对连作党参根腐病抗性及生理特性的影响[J].干旱地区农业研究,2025,(5):234~243 |
| 外源物质预处理对连作党参根腐病抗性及生理特性的影响 |
| Effects of exogenous substance pretreatment on root rot resistance and physiological characteristics of continuous cropping Codonopsis pilosula |
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| DOI：10.7606/j.issn.1000-7601.2025.05.23 |
| 中文关键词: 党参 根腐病 外源调控 抑菌率 生理特性 |
| 英文关键词:Codonopsis pilosula root rotdisease exogenous regulation bacteriostatic rate physiological characteristics |
| 基金项目:国家自然科学基金（31960395） |
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| 中文摘要: |
| 为探究3种外源物质缓解连作党参根腐病的作用机制，本研究以盆栽1年生党参幼苗为材料，设置空白对照(CK1)、病原菌侵染(CK2)、28-高芸苔素内酯灌根（T1）及叶喷(T2)、枯草芽孢杆菌(J1)和解淀粉芽孢杆菌(J2)接种及氨基酸水溶肥喷施(Fa)处理，30 d后接种病原菌，测定外源物质抑菌率及叶片生理生化指标变化。结果表明：J1和J2处理显著抑制病原菌侵染钉生长，抑菌率均超70%；相较于CK2，T1、T2和Fa处理显著提高叶绿素a含量（39.85%~81.52%），叶绿素b含量（68.94%~168.45%），并增强过氧化物酶(POD)活性（81.33%~95.33%）和过氧化氢酶(CAT)活性（8.91%~39.36%），同时有效减轻细胞膜损伤和脂质过氧化；J1和J2处理则显著降低超氧化物歧化酶(SOD)活性16.85%和13.76%、丙二醛(MDA)含量47.83%和 50.44%、脯氨酸含量20.55%和18.23%、可溶性糖含量35.82%和35.01%、可溶性蛋白含量35.46%和35.21%，使这些胁迫指标恢复至接近正常水平。综上，生防菌可有效抑制病原菌菌丝的生长及孢子萌发；生长调节剂和氨基酸水溶肥可平衡渗透调节系统，增强抗氧化防御机制，促进光合作用，增强植物抗逆性，从而缓解病害。 |
| 英文摘要: |
| In order to explore the mechanism of three exogenous substances to alleviate the root rot of Codonopsis pilosula, potted annual C.pilosula seedlings were used as materials in this study. Blank control (CK1), pathogen infection (CK2), 28-homobrassinolide root irrigation (T1), and leaf spray (T2), Bacillus subtilis (J1), and Bacillus amyloliquebilis (J2) were inoculated, and amino acid water\|soluble fertilizer spraying (Fa) treatments were set up. The pathogenic bacteria were inoculated 30 days later, and the inhibition rate of exogenous substances and the changes of physiological and biochemical indexes of leaves were measured. The results showed that J1 and J2 treatments significantly inhibited the growth of pathogenic infected nails, and the inhibition rate was over 70%. Compared with CK2, T1, T2, and Fa treatments significantly increased the contents of chlorophyll a (39.85%~81.52%) and chlorophyll b (68.94%~168.45%) and enhanced the activities of peroxidase (POD) (81.33%~95.33%) and catalase (CAT) (8.91%~39.36%), while effectively reducing cell membrane damage and lipid peroxidation. J1 and J2 treatments significantly reduced the activities of superoxide dismutase (SOD) by 16.85% and 13.76%, malondialdehyde (MDA) by 47.83% and 50.44%, proline by 20.55% and 18.23%, soluble sugar by 35.82% and 35.01%, and soluble protein by 35.46% and 35.21%, respectively, and these stress indexes returned to near\|normal levels. In conclusion, biocontrol bacteria can effectively inhibit the growth of pathogenic mycelium and spore germination. Growth regulators and amino acid water\|soluble fertilizers can balance the osmoregulatory system, enhance antioxidant defense mechanisms, promote photosynthesis, and enhance plant stress resistance, thereby alleviating diseases. |
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