| 夏玉侨,荆瑞勇,姜雪,王丽艳,郭永霞,于镇华,刘俊杰.大豆根内耐盐碱促生细菌的分离鉴定及其促生功能研究[J].干旱地区农业研究,2025,(5):244~251 |
| 大豆根内耐盐碱促生细菌的分离鉴定及其促生功能研究 |
| Isolation and identification of endophytic salt\|alkali tolerant growth\|promoting bacteria from soybean roots and their growth\|promoting functions |
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| DOI：10.7606/j.issn.1000-7601.2025.05.24 |
| 中文关键词: 大豆；根内生细菌；耐盐碱 分离鉴定；促生功能 |
| 英文关键词:soybean root endophytic bacteria salt\|alkai tolerance isolation and identification growth\|promoting function |
| 基金项目:中国科学院战略性先导科技专项：黑土地保护与利用科技创新工程专项（XDA28100200）；黑龙江八一农垦大学三纵（基础培育）项目（ZRCPY202218） |
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| 中文摘要: |
| 为挖掘苏打盐碱地大豆根内生细菌资源，以大庆苏打盐碱地大豆根系为材料，以组织研磨、平板划线和PCR测序法对大豆根内生细菌进行分离纯化鉴定，并测定其解磷、固氮、产铁载体、ACC脱氨酶、IAA能力、耐苏打盐碱能力；同时在浸种条件下进行大豆发芽试验。结果表明：筛选出的具有解磷、固氮、产铁载体、产IAA、产ACC脱氨酶、耐受苏打盐碱（100~150 mmoL·L-1 NaHCO3和Na2CO3，摩尔比为9∶1；pH值8.8）能力的3株内生细菌鉴定为暹罗芽孢杆菌（Bacillus siamensis DC.24）、维氏芽孢杆菌（Bacillus wiedmannii DM57）、栖木假单胞菌（Pseudomonas hibiscicola DC-2）。发芽试验使用3株根内生细菌浸种处理大豆，均可显著提高大豆根尖数、根长、根表面积、根体积。在‘合丰50’大豆品种中接种上述3株菌,大豆幼苗根尖数分别增加169.7%、221.2%、172.7%，根长分别增加52.8%、63.0%、29.8%，根体积分别增加139.9%、125.6%、96.9%，根表面积分别增加89.2%、83.6%、58.7%；接种于‘龙垦310’品种,使其幼苗根尖数分别增加758.8%、329.4%、341.1%，根长分别增加101.1%、52.2%、50.0%，根体积分别增加129.4%、82.3%、70.5%，根表面积分别增加92.6%、51.2%、41.4%，表明3株根内生细菌具有促生功能，可作为开发苏打盐碱地微生物肥的菌种。 |
| 英文摘要: |
| In order to explore the endophytic bacteria resources of soybean roots in soda saline\|alkali land, the soybean roots in Daqing soda saline\|alkali land were used as materials. The endophytic bacteria in soybean roots were isolated, purified, and identified by tissue grinding, plate streaking, and PCR sequencing, and their phosphate solubilization, nitrogen fixation, siderophore production, ACC deaminase, IAA ability, and soda saline\|alkali tolerance were determined. The soybean germination test was carried out under soaking conditions. The results showed that the three screened endophytic bacteria that had the functions of phosphate solubilization, nitrogen fixation, siderophore production, plant hormone IAA production, ACC deaminase production, and tolerance to soda saline\|alkali (100~150 mmoL·L-1 NaHCO3 and Na2CO3, molar ratio of 9∶1; pH 8.8) were identified as Bacillus siamensis DC.24, Bacillus wiedmannii DM57, and Pseudomonas hibiscicola DC-2. In the germination test, the number of root tips, root length, root surface area, and root volume of soybean were significantly increased by soaking seeds with three strains of endophytic bacteria. Inoculation of the three endophytic bacteria into the ‘Hefeng 50’ soybean variety resulted in an increase of 169.7%, 221.2%, and 172.7% in root tip number, 52.8%, 63.0%, and 29.8% in root length, 139.9%, 125.6%, and 96.9% in root volume, and 89.2%, 83.6%, and 58.7% in root surface area, respectively. In the ‘Longken 310’ variety, the number of root tips increased by 758.8%, 329.4%, and 341.1%, root length increased by 101.1%, 52.2%, and 50.0%, root volume increased by 129.4%, 82.3%, and 70.5%, and root surface area increased by 92.6%, 51.2%, and 41.4%, respectively. This indicated that the three root endophytic bacteria have growth\|promoting functions and need to be developed for soda saline\|alkali soil microbial fertilizer. |
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