| 郝军卫,何卓恒,罗爽,杨文琪,聂小军.237份小麦穗部性状的全基因组关联分析[J].干旱地区农业研究,2025,(6):1~11 |
| 237份小麦穗部性状的全基因组关联分析 |
| Genome\|wide association study of spike\|related traits in 237 wheat accessions |
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| DOI：10.7606/j.issn.1000-7601.2025.06.01 |
| 中文关键词: 小麦 全基因组关联分析 穗部性状 候选基因 |
| 英文关键词:wheat genome\|wide association study spike\|related traits candidate genes |
| 基金项目:农业农村部农业生物育种重大专项（2023ZD0402601）；国家自然科学基金项目（U22A20457） |
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| 中文摘要: |
| 以237份（185份ICARDA引进种质和52份国内核心种质）小麦种质为材料，对其在8个环境下的穗长、单株穗数、每穗穗粒数、每穗小穗数等4个性状进行调查，发现其表现出广泛的变异，其中穗长变异幅度最小（9.63%~13.22%），每穗穗粒数变异幅度最大（9.65%~25.45%）；结合660K SNP芯片进行基因分型，基于多位点随机SNP效应混合线性模型对4个性状进行全基因组关联分析，共发掘到显著性位点506个，其中穗长相关的148个、单株穗数相关的107个、每穗穗粒数相关的138个、每穗小穗数相关的113个；结合LD和基因组注释，鉴定到490个穗长相关候选基因、164个单株穗数候选基因、181个每穗穗粒数候选基因以及81个每穗小穗数候选基因，其中37个显著SNP位点位于基因内部，是潜在的关键候选基因；结合单倍型分析，最终确定TraesCS1A02G285000、TraesCS4D02G029400、TraesCS2D02G155000、TraesCS1D02G383400、TraesCS5B02G274700、TraesCS6A02G002500、TraesCS2D02G018300等7个基因为控制小麦穗部性状的关键候选基因。本研究为利用外来引进种质以克服小麦育种的遗传同质化、培育高产小麦新品种提供了材料和基因资源。 |
| 英文摘要: |
| In this study, 237 wheat germplasms (185 ICARDA\|introduced wheat accessions and 52 domestic core germplasms) were used as the materials to investigate the four traits of spike length, number of spikes per plant, number of grains per spike, and number of spikelets per spike under eight environmental conditions. The results showed that the four spike\|related traits exhibited extensive variation across the eight environments, with the minimum variation range of spike length (9.63% to 13.22%) and the maximum variation range of spike grain number per spike (9.65% to 25.45%). Then, GWAS of four spike\|related traits were performed using a multi\|locus random SNP effect mixed linear model based on the genotyping data from a 660K SNP array analysis. A total of 506 significant loci associated with spike traits were identified, including 148 for spike length, 107 for number of spikes per plant, 138 for number of grains per spike, and 113 for number of spikelets per spike. Based on LD and genome annotation information, 490 candidate genes associated with spike length were obtained, as well as 164 genes associated with the number of spikes per plant, 181 genes associated with the number of grains per spike, and 81 genes associated with the number of spikelets per spike, of which 37 associated SNPs were found in its genetic regions. Based on haplotype analysis, we ultimately obtained seven main candidate genes, namely TraesCS1A02G285000, TraesCS4D02G029400, TraesCS2D02G155000, TraesCS1D02G383400, TraesCS5B02G274700, TraesCS6A02G002500, and TraesCS2D02G018300. This study provided the material and genetic resources for overcoming genetic homogenization and breeding high\|yield new wheat varieties through utilizing the introduced germplasm. |
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