| 韩东辰,陈伟,牛欣玥.黑果枸杞对风沙胁迫的生理响应特征分析[J].干旱地区农业研究,2025,(6):47~56 |
| 黑果枸杞对风沙胁迫的生理响应特征分析 |
| Analysis of physiological response characteristics of Lycium ruthenicum to wind\|sand stress |
| |
| DOI：10.7606/j.issn.1000-7601.2025.06.05 |
| 中文关键词: 黑果枸杞 风沙胁迫 光合生理 抗氧化酶 相对含水量 西北地区 |
| 英文关键词:Lycium ruthenicum wind\|sand stress photosynthetic physiology antioxidant enzyme relative water content northwest region |
| 基金项目:国家自然科学基金项目（31701949）；山西省中央引导发展专项（YDZJSX20231A052）；山西省教育科学“十四五”规划资助项目（GH-221031） |
|
| 摘要点击次数: 270 |
| 全文下载次数: 63 |
| 中文摘要: |
| 为探究黑果枸杞对风沙胁迫的生理响应特征，选取甘肃民勤青土湖区黑果枸杞种苗，在风洞实验室模拟净风和风沙流胁迫，并测定光合生理指标、抗氧化酶活性、渗透调节物质含量、膜脂过氧化程度和叶片气孔指标。结果表明：净风胁迫下，Pn、Gs、Ci和Tr在风速12 m·s-1时达到峰值，分别38.3 μmol·m-2·s-1、0.48 mol·m-2·s-1、8.01 μmol·mol-1和8.2 mmol·m-2·s-1；SOD活性在5 m·s-1时达到峰值42.3 U·g-1，POD活性在15 m·s-1时达到峰值39.6 U·g-1·min-1；CAT活性在12 m·s-1时达到峰值9.1 U·g-1·min-1，脯氨酸含量在12 m·s-1时达到峰值207 μg·g-1，MDA含量在12 m·s-1时达到峰值7.6 nmol·g-1，上表皮气孔密度在5 m·s-1时达到峰值5 216个·mm-2，下表皮气孔密度在12 m·s-1时达到峰值3 368个·mm-2。风沙流胁迫下，Pn、Gs、Ci和Tr随胁迫时间增加而上升，在24 min时达到峰值，分别为19.1 μmol·m-2·s-1、0.95 mol·m-2·s-1、16.3 μmol·mol-1和17.7 mmol·m-2·s-1；SOD活性在8 min时达到峰值36.2 U·g-1；POD活性在12 m·s-1时达到峰值65.6 U·g-1·min-1；CAT活性在8 min时达到峰值10.8 U·g-1·min-1；脯氨酸含量在16 min时达到峰值231 μg·g-1；可溶性糖含量在12 m·s-1时达到峰值8.0 mg·g-1；MDA含量在8 m·s-1时达到峰值9.4 nmol·g-1；上表皮气孔密度在5 m·s-1时达到峰值3 986个·mm-2，下表皮气孔密度在12 m·s-1时达到峰值4 581个·mm-2。研究表明，黑果枸杞对风沙胁迫的生理响应特征具有明显的风速和胁迫时间依赖性，植物通过动态调节光合生理、抗氧化酶活性、渗透调节物质含量和气孔指标等生理过程，以适应不同强度和持续时间的风沙胁迫。 |
| 英文摘要: |
| In order to explore the physiological response characteristics of Lycium ruthenicum to wind\|sand stress, L. ruthenicum seedlings from Qingtu Lake Area, Minqin, Gansu Province, were selected for the study. The stress of clean wind and wind\|sand flow was simulated in the wind tunnel laboratory, and the photosynthetic physiological indicators, antioxidant enzyme activities, contents of osmotic regulatory substances, degree of membrane lipid peroxidation, and leaf stomatal indicators were determined. The results showed that under clean air stress, Pn, Gs, Ci, and Tr reach their peaks at 12 m·s-1, which are 38.3 μmol·m-2·s-1, 0.48 mol·m-2·s-1, 8.01 μmol·mol-1 and 8.2 mmol·m-2·s-1, respectively. The SOD activity reached a peak of 42.3 U·g-1 at 5 m·s-1, and the POD activity reached a peak of 39.6 U·g-1·min-1 at 15 m·s-1. The CAT activity reached a peak of 9.1 U·g-1·min-1 at 12 m·s-1 under clean air stress, the proline content reached a peak of 207 μg·g-1 at 12 m·s-1 under clean air stress, and the MDA content reached a peak of 7.6 nmol·g-1 at 12 m·s-1 under clean air stress. The stomatal density of the upper epidermis reached a peak of 5 216 PCS·mm-2 at 5 m·s-1, and that of the lower epidermis reached a peak of 3 368 PCS·mm-2 at 12 m·s-1. Under the stress of wind\|sand flow, Pn, Gs, Ci, and Tr increased with the increase of stress time and reached the peak at 24 minutes. They were 19.1 μmol·m-2·s-1, 0.95 mol·m-2·s-1, 16.3 μmol·mol-1, and 17.7 mmol·m-2·s-1, respectively. The SOD activity reached a peak of 36.2 U·g-1 at 8 min. The POD activity reached a peak of 65.6 U·g-1·min-1 at 12 m·s-1. The CAT activity reached the peak of 10.8 U·g-1·min-1 at 8 min. The proline content reached a peak of 231 μg·g-1 at 16 min. The content of soluble sugar reached a peak of 8.0 mg·g-1 at 12 m·s-1. The MDA content reached a peak of 9.4 nmol·g-1 at 8 m·s-1. The stomatal density of the upper epidermis reached a peak of 3 986 PCS·mm-2 at 5 m·s-1, and that of the lower epidermis reached a peak of 4 581 PCS·mm-2 at 12 m·s-1. The research results showed that the physiological response characteristics of L. ruthenicum to wind\|sand stress are significantly dependent on wind speed and stress time. Plants adapt to wind\|sand stress of different intensities and durations by dynamically regulating physiological processes such as photosynthetic physiology, antioxidant enzyme activity, osmotic regulatory substance content, and stomatal indicators. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| | |