| 巴广,宫延静,柳海燕,邓霏,王红明.甜樱桃HD-Zip基因家族鉴定及胁迫条件下的表达分析[J].干旱地区农业研究,2025,(6):77~87 |
| 甜樱桃HD-Zip基因家族鉴定及胁迫条件下的表达分析 |
| Identification of HD-Zip gene family in Prunusavium L. and expression analysis under stress conditions |
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| DOI：10.7606/j.issn.1000-7601.2025.06.08 |
| 中文关键词: 甜樱桃 HD-Zip转录因子 基因表达 非生物胁迫 |
| 英文关键词:Prunusavium L. HD-Zip transcription factor gene expression abiotic stress |
| 基金项目:天水师范学院产业支撑引导项目(CYZ2022-02)；天水市秦州区科学技术局科学技术研究与开发项目（2023-NCKJG-1058） |
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| 中文摘要: |
| 同源异型域亮氨酸拉链(HD-Zip，homeodomain\|leucine zipper)蛋白对调控植物生长发育及响应逆境胁迫至关重要。运用生物信息学方法对甜樱桃（Prunusavium L.）HD-Zip基因家族成员进行鉴定，对其理化性质、二级结构、保守基序、系统进化、启动子元件和基因结构等进行分析。基于转录组数据挖掘HD-Zip家族成员组织特异性及胁迫条件下的表达模式,通过qRT-PCR检测HD-Zip基因在甜樱桃不同组织中的表达量。结果表明：HD-Zip家族共有15个成员，编码氨基酸数在127~837 aa之间，预测大多数成员定位在细胞核中，HD-Zip家族蛋白均属亲水性不稳定蛋白，HD-Zip基因结构比较复杂，外显子数在1~18之间，不同家族成员中分布不同的保守基序。系统发育树显示，HD-Zip家族成员分为4个亚族，且同一亚族成员间具有相似的基因结构组成和保守基序。HD-Zip基因启动子区存在生长素、赤霉素和茉莉酸等植物激素及多种逆境胁迫响应顺式作用元件。转录组结果显示，PavHB7A在果实和果蒂中特异表达，PavHB7B在花中的表达水平显著高于其他组织，最高可达200倍；PavHB15在当年生新梢中的表达量高于成熟叶片，可达27倍。qRT-PCR结果表明，HD-Zip基因在芽、根、茎、叶和花中的表达量存在差异。干旱处理后，PavHB7A在‘马哈利’根和叶中的表达量上调，分别增加665.6%和365.9%，表明PavHB7A基因参与干旱胁迫的应答过程。 |
| 英文摘要: |
| Homodomain\|leucine zipper (HD-Zip) proteins are essential for regulating plant growth and development and responding to adversity stress. Bioinformatics methods were used to identify the sweet cherry (Prunusavium L.) HD-Zip gene family members and analyze their physicochemical properties, secondary structure, conserved motifs, phylogeny, promoter elements, and gene structure. Based on the transcriptome data, the HD-Zip family members were mined for tissue specificity and expression patterns under stress conditions; meanwhile, the expression of HD-Zip genes in different tissues of sweet cherry was detected by qRT-PCR. The results showed that there are 15 members of the HD-Zip family, coding for amino acids ranging from 127 aa to 837 aa, and most of the members are predicted to be localized in the nucleus. The HD-Zip family proteins were hydrophilic and unstable proteins, the structure of the HD-Zip gene was relatively complex, the number of exons ranged from 1 to 18, and different conserved motifs were distributed in different family members. The phylogenetic tree showed that the HD-Zip family members were divided into four subfamilies, and the members of the same subfamily had similar structural composition and conserved motifs. There were cis\|acting elements in the promoter region of HD-Zip genes for phytohormones such as growth hormone, gibberellin, and jasmonic acid, as well as for a variety of adversity stresses. The transcriptome results showed that PavHB7A was specifically expressed in fruits and pedicels, and the expression level of PavHB7B in flowers was significantly higher than that in other tissues, up to 200 times. The expression level of PavHB15 in young shoots was 27 times higher than that in mature leaves. qRT-PCR results showed that HD-Zip genes were differentially expressed in buds, roots, stems, leaves, and flowers. After drought treatment, the expression of PavHB7A was up\|regulated in the roots and leaves of ‘CDR-1’, which increased by 665.6% and 365.9%, respectively, indicating that the PavHB7A gene was involved in the response process of drought stress. |
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