| 王雪玮,王宝强,朱晓林,桂金凤,陈立飞,魏小红.藜麦DOF基因家族鉴定及NO调控干旱胁迫下表达模式分析[J].干旱地区农业研究,2026,(1):31~40 |
| 藜麦DOF基因家族鉴定及NO调控干旱胁迫下表达模式分析 |
| Identification of the DOF gene family in quinoa (Chenopodium quinoa) and analysis of expression patterns regulated by nitric oxide (NO) under drought stress |
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| DOI：10.7606/j.issn.1000-7601.2026.01.04 |
| 中文关键词: 藜麦 DOF基因家族 NO 干旱胁迫 生物信息学 表达模式 |
| 英文关键词:quinoa DOF gene family NO (nitric oxide) drought stress bioinformatics expression pattern |
| 基金项目:国家自然科学基金（32060401）；甘肃省自然科学基金（23JRRA1426） |
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| 中文摘要: |
| DOF（DNA binding with one zinc finger）是植物特异性转录因子，在植物抵御逆境胁迫过程中发挥着关键作用。通过生物信息学方法对藜麦DOF基因家族成员进行全基因组鉴定，并结合qRT-PCR技术解析该基因家族在外源NO调控干旱胁迫下的表达模式。结果表明，藜麦DOF基因家族共鉴定出25个家族成员，其氨基酸长度为143~558 aa，分子质量为16 079.9~59 102.2 Da，等电点介于4.52~9.60，疏水指数小于2，预测亚细胞定位均定位在细胞核；系统发育分析将DOF基因家族分为5个亚族，25个DOF基因均匀分布在13条染色体上，6对串联重复基因；启动子区共有31个与光响应、组织特异性、压力响应及植物激素相关的顺式作用元件；DOF5.3是蛋白互作网络的核心节点。不同组织器官表达分析表明，CqDOFs在藜麦各组织中均有表达且在花和叶中表达较强，在不同非生物胁迫条件下大部分CqDOFs基因在根中强烈响应；qRT-PCR分析表明，在NO调控的干旱胁迫条件下，16个CqDOFs基因的相对表达量均明显升高，其中CqDOF10在NO调控的干旱胁迫下表达量为干旱胁迫下该基因表达量的215.3倍，表明CqDOFs基因在藜麦响应干旱胁迫的调控机制中发挥着重要作用，外源NO能有效提高干旱胁迫下基因的表达。 |
| 英文摘要: |
| DOF (DNA binding with one zinc finger) is a plant\|specific transcription factor that plays a crucial role in plants’ response to abiotic stress. Through bioinformatics methods, the DOF gene family members in quinoa were identified genome\|wide, and the expression patterns of this gene family under drought stress regulated by exogenous NO were analyzed using qRT-PCR technology. The results showed that a total of 25 family members of the quinoa DOF gene family were identified, with amino acid lengths ranging from 143 to 558 aa, molecular weights ranging from 16 079.9 to 59 102.2 Da, isoelectric points ranging from 4.52 to 9.60, hydrophobic indices less than 2, and all predicted to be located in the nucleus. Phylogenetic analysis divided the DOF gene family into five subfamilies. The 25 DOF genes were evenly distributed on 13 chromosomes, with 6 pairs of tandem repeat genes. There were 31 cis\|acting elements related to light response, tissue specificity, stress response, and plant hormones in the promoter region. DOF5.3 was the core node of the protein interaction network. Expression analysis in different tissues and organs indicated that CqDOFs were expressed in all quinoa tissues, with stronger expression in flowers and leaves. Under different abiotic stress conditions, most CqDOFs genes responded strongly in roots. qRT-PCR analysis showed that under drought stress regulated by exogenous NO, the relative expression levels of 16 CqDOFs genes significantly increased. Among them, the expression level of CqDOF10 under drought stress regulated by NO was 215.3 times that under drought stress alone, indicating that CqDOFs genes play an important role in the regulatory mechanism of quinoa’s response to drought stress, and exogenous NO can effectively increase the expression of genes under drought stress. |
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